KEGG   ORTHOLOGY: K16083
Entry
K16083                      KO                                     
Symbol
CYP71Z6
Name
ent-isokaurene hydroxylase [EC:1.14.14.76]
Pathway
map00904  Diterpenoid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R09861  ent-isokaurene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase
R11978  ent-isokaurene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K16083  CYP71Z6; ent-isokaurene hydroxylase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K16083  CYP71Z6; ent-isokaurene hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.76  ent-isokaurene C2/C3-hydroxylase
     K16083  CYP71Z6; ent-isokaurene hydroxylase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP71 family
   K16083  CYP71Z6; ent-isokaurene hydroxylase
Other DBs
GO: 0036201
Genes
OSA: 4329725(CYP71Z6)
OGL: 127761677
Reference
PMID:21985968
  Authors
Wu Y, Hillwig ML, Wang Q, Peters RJ
  Title
Parsing a multifunctional biosynthetic gene cluster from rice: Biochemical characterization of CYP71Z6 & 7.
  Journal
FEBS Lett 585:3446-51 (2011)
DOI:10.1016/j.febslet.2011.09.038
  Sequence
[osa:4329725]
LinkDB

KEGG   ENZYME: 1.14.14.76
Entry
EC 1.14.14.76               Enzyme                                 
Name
ent-isokaurene C2/C3-hydroxylase;
CYP71Z6;
ent-isokaurene C2-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
ent-isokaurene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (ent-isokaurene-2beta,3beta-diol-forming)
Reaction(IUBMB)
ent-isokaurene + 2 O2 + 2 [reduced NADPH---hemoprotein reductase] = ent-isokaurene-2beta,3beta-diol + [oxidized NADPH---hemoprotein reductase] + 2 H2O (overall reaction) [RN:R12058];
(1a) ent-isokaurene + O2 + [reduced NADPH---hemoprotein reductase] = ent-isokauren-2beta-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09861];
(1b) ent-isokauren-2beta-ol + O2 + [reduced NADPH---hemoprotein reductase] = ent-isokaurene-2beta,3beta-diol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11978]
Reaction(KEGG)
R09861 R11978 R12058
Substrate
ent-isokaurene [CPD:C20145];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
ent-isokauren-2beta-ol [CPD:C20146]
Product
ent-isokaurene-2beta,3beta-diol [CPD:C21831];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
ent-isokauren-2beta-ol [CPD:C20146]
Comment
This cytochrome P-450 (heme thiolate) enzyme has been characterized from the plant Oryza sativa (rice). It may be involved in production of oryzadione.
History
EC 1.14.14.76 created 2012 as EC 1.14.13.143, transferred 2018 to EC 1.14.14.76
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K16083  ent-isokaurene hydroxylase
Genes
OSA: 4329725(CYP71Z6)
OGL: 127761677
Reference
1  [PMID:21985968]
  Authors
Wu Y, Hillwig ML, Wang Q, Peters RJ
  Title
Parsing a multifunctional biosynthetic gene cluster from rice: Biochemical characterization of CYP71Z6 & 7.
  Journal
FEBS Lett 585:3446-51 (2011)
DOI:10.1016/j.febslet.2011.09.038
  Sequence
[osa:4329725]
Reference
2  [PMID:25758958]
  Authors
Kitaoka N, Wu Y, Xu M, Peters RJ
  Title
Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis.
  Journal
Appl Microbiol Biotechnol 99:7549-58 (2015)
DOI:10.1007/s00253-015-6496-2
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.76
IUBMB Enzyme Nomenclature: 1.14.14.76
ExPASy - ENZYME nomenclature database: 1.14.14.76
BRENDA, the Enzyme Database: 1.14.14.76
LinkDB

KEGG   REACTION: R09861
Entry
R09861                      Reaction                               
Name
ent-isokaurene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase
Definition
ent-Isokaurene + Oxygen + [Reduced NADPH---hemoprotein reductase] <=> ent-2alpha-Hydroxyisokaurene + H2O + [Oxidized NADPH---hemoprotein reductase]
Equation
C20145 + C00007 + C03024 <=> C20146 + C00001 + C03161
Comment
the first step of two-step reaction (see R12058, R09861+R11978)
Reaction class
RC00478  C20145_C20146
Enzyme
1.14.14.76
Pathway
rn00904  Diterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.76
     R09861  ent-Isokaurene + Oxygen + [Reduced NADPH---hemoprotein reductase] <=> ent-2alpha-Hydroxyisokaurene + H2O + [Oxidized NADPH---hemoprotein reductase]
Orthology
K16083  ent-isokaurene hydroxylase [EC:1.14.14.76]
Other DBs
RHEA: 31950
LinkDB

DBGET integrated database retrieval system