KEGG   ORTHOLOGY: K16968
Entry
K16968                      KO                                     
Symbol
msmA
Name
methanesulfonate monooxygenase subunit alpha [EC:1.14.13.111]
Pathway
map00920  Sulfur metabolism
map01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00920 Sulfur metabolism
    K16968  msmA; methanesulfonate monooxygenase subunit alpha
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.111  methanesulfonate monooxygenase (NADH)
     K16968  msmA; methanesulfonate monooxygenase subunit alpha
Other DBs
RN: R09513
GO: 0018648
Genes
PQI: KH389_17555
CUH: BJN34_09325 BJN34_22320
BCT: GEM_5812
PPK: U875_05270
PPNO: DA70_22340
PRB: X636_04325
BPA: BPP0512
BBH: BN112_2904
BBR: BB0517
BBM: BN115_0496
BTRM: SAMEA390648701717(antA)
PUT: PT7_2466
MPT: Mpe_A0919(msmS2)
FIL: BN1229_v1_3549(msmA)
FIY: BN1229_v1_2374(msmA)
AG: AAD26619(msmA)
 » show all
Reference
  Authors
de Marco P, Moradas-Ferreira P, Higgins TP, McDonald I, Kenna EM, Murrell JC
  Title
Molecular analysis of a novel methanesulfonic acid monooxygenase from the methylotroph Methylosulfonomonas methylovora.
  Journal
J Bacteriol 181:2244-51 (1999)
DOI:10.1128/JB.181.7.2244-2251.1999
  Sequence
Reference
  Authors
Baxter NJ, Scanlan J, De Marco P, Wood AP, Murrell JC
  Title
Duplicate copies of genes encoding methanesulfonate monooxygenase in Marinosulfonomonas methylotropha strain TR3 and detection of methanesulfonate utilizers in the environment.
  Journal
Appl Environ Microbiol 68:289-96 (2002)
DOI:10.1128/AEM.68.1.289-296.2002
LinkDB

KEGG   ORTHOLOGY: K16969
Entry
K16969                      KO                                     
Symbol
msmB
Name
methanesulfonate monooxygenase subunit beta [EC:1.14.13.111]
Pathway
map00920  Sulfur metabolism
map01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00920 Sulfur metabolism
    K16969  msmB; methanesulfonate monooxygenase subunit beta
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.111  methanesulfonate monooxygenase (NADH)
     K16969  msmB; methanesulfonate monooxygenase subunit beta
Other DBs
RN: R09513
GO: 0018648
Genes
PQI: KH389_17550
CUH: BJN34_09330
BCT: GEM_5811
BUE: BRPE67_FCDS01800
PPK: U875_05275
PPNO: DA70_22345
PRB: X636_04320
BPA: BPP0513
BBH: BN112_2903
BBR: BB0518
PUT: PT7_2465
MPT: Mpe_A0920(msmB)
FIL: BN1229_v1_3548(msmB)
FIY: BN1229_v1_2375(msmB)
AG: AAD26620(msmB)
 » show all
Reference
  Authors
de Marco P, Moradas-Ferreira P, Higgins TP, McDonald I, Kenna EM, Murrell JC
  Title
Molecular analysis of a novel methanesulfonic acid monooxygenase from the methylotroph Methylosulfonomonas methylovora.
  Journal
J Bacteriol 181:2244-51 (1999)
DOI:10.1128/JB.181.7.2244-2251.1999
  Sequence
Reference
  Authors
Baxter NJ, Scanlan J, De Marco P, Wood AP, Murrell JC
  Title
Duplicate copies of genes encoding methanesulfonate monooxygenase in Marinosulfonomonas methylotropha strain TR3 and detection of methanesulfonate utilizers in the environment.
  Journal
Appl Environ Microbiol 68:289-96 (2002)
DOI:10.1128/AEM.68.1.289-296.2002
LinkDB

KEGG   ORTHOLOGY: K15762
Entry
K15762                      KO                                     
Symbol
tmoC, tbuB, touC
Name
toluene monooxygenase system ferredoxin subunit
Pathway
map00623  Toluene degradation
map00920  Sulfur metabolism
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01220  Degradation of aromatic compounds
Module
M00538  Toluene degradation, toluene => benzoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00920 Sulfur metabolism
    K15762  tmoC, tbuB, touC; toluene monooxygenase system ferredoxin subunit
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K15762  tmoC, tbuB, touC; toluene monooxygenase system ferredoxin subunit
Other DBs
RN: R02550 R03562 R05666 R09513
Genes
PSD: DSC_04395
MCA: MCA0225 MCA0586
METU: GNH96_03875 GNH96_15290
MMAI: sS8_4250 sS8_5417 sS8_5458
CZA: CYCME_2096
THIS: HZT40_03315
HAZ: A9404_01470
SINI: GT972_13800
TBN: TBH_C0917
VFF: VITFI_CDS2832
RPI: Rpic_4634
CNC: CNE_BB1p12340(tbcC)
RME: Rmet_1314(tmoC)
BCON: NL30_24545
BUG: BC1001_PA6408(tmoC)
BHO: D560_1527
BHM: D558_1518
PNA: Pnap_2045
HPSE: HPF_01795(tmoC)
MPT: Mpe_A0816(tbuB) Mpe_A0922(msmC) Mpe_A2541(tbuB)
DAR: Daro_3818
AZO: azo2601
AOA: dqs_2762
ACIB: ACBT_0271(tmoC)
BRA: BRADO3598
XAU: Xaut_4301
BID: Bind_2847
HMC: HYPMC_2243(tmoC)
MSC: BN69_2679
BTS: Btus_2067
SAY: TPY_0637
MSHG: MSG_02602
MSTO: MSTO_08240
MSAK: MSAS_06400
MPHL: MPHLCCUG_04539(tmoC)
MHAS: MHAS_04835(tmoC)
MMAG: MMAD_01690(nirD)
MMOR: MMOR_05350
MANY: MANY_23690
MTER: 4434518_03548(tmoC)
MHIB: MHIB_29680
CCYC: SCMU_04680
NFR: ERS450000_00317(tmoC)
TRO: trd_A0921
SSO: SSO1230(tmoC)
SOL: Ssol_2198
SSOA: SULA_2231
SSOL: SULB_2232
SSOF: SULC_2229
SID: M164_0422
SII: LD85_0413
SIH: SiH_0429
SIR: SiRe_0387
PAS: Pars_0544
AG: AAA26001(tmoC)
 » show all
Reference
PMID:1885512
  Authors
Yen KM, Karl MR, Blatt LM, Simon MJ, Winter RB, Fausset PR, Lu HS, Harcourt AA, Chen KK
  Title
Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 173:5315-27 (1991)
DOI:10.1128/JB.173.17.5315-5327.1991
  Sequence
Reference
PMID:1429451
  Authors
Yen KM, Karl MR
  Title
Identification of a new gene, tmoF, in the Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 174:7253-61 (1992)
DOI:10.1128/JB.174.22.7253-7261.1992
LinkDB

KEGG   ORTHOLOGY: K15765
Entry
K15765                      KO                                     
Symbol
tmoF, tbuC, touF
Name
toluene monooxygenase electron transfer component [EC:1.18.1.3]
Pathway
map00623  Toluene degradation
map00920  Sulfur metabolism
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01220  Degradation of aromatic compounds
Module
M00538  Toluene degradation, toluene => benzoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00920 Sulfur metabolism
    K15765  tmoF, tbuC, touF; toluene monooxygenase electron transfer component
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K15765  tmoF, tbuC, touF; toluene monooxygenase electron transfer component
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.18  Acting on iron-sulfur proteins as donors
   1.18.1  With NAD+ or NADP+ as acceptor
    1.18.1.3  ferredoxin---NAD+ reductase
     K15765  tmoF, tbuC, touF; toluene monooxygenase electron transfer component
Other DBs
RN: R02550 R03562 R05666 R09513
Genes
PSD: DSC_04380
PPSE: BN5_3604
MBS: MRBBS_1308(tmoF)
MSR: AU15_20760
CYQ: Q91_2220
CZA: CYCME_2538
CYY: CPC19_01700
THIS: HZT40_03300
APAC: S7S_13335
MGEO: CFI10_14575 CFI10_17775
TBN: TBH_C0920
RPI: Rpic_4637
CNC: CNE_BB1p12310(tmoF)
RME: Rmet_1311
BCT: GEM_5810
BCON: NL30_24560
BUG: BC1001_PA6405(tmoF)
PPNO: DA70_22375
BPE: BP0066
BPC: BPTD_0064
BPER: BN118_3398
BPET: B1917_0069
BPEU: Q425_690
BPA: BPP0517
BBR: BB0522
BTRM: SAMEA390648701722(dmpP_1)
PUT: PT7_2459
LIM: L103DPR2_00189(carAd)
HPSE: HPF_01780(tmoF)
MPT: Mpe_A0819(tbuC) Mpe_A0921(msmD)
DAR: Daro_3815
BBT: BBta_1593
AOL: S58_10620
OCA: OCAR_6789
FIL: BN1229_v1_3546(msmD)
FIY: BN1229_v1_2377(msmD)
HDI: HDIA_4247(carAd)
SGI: SGRAN_2803(thnA4)
BTS: Btus_2064
NFR: ERS450000_00316(tmoF)
TRO: trd_A0924
AG: AAA26004(tmoF)
 » show all
Reference
PMID:1885512
  Authors
Yen KM, Karl MR, Blatt LM, Simon MJ, Winter RB, Fausset PR, Lu HS, Harcourt AA, Chen KK
  Title
Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 173:5315-27 (1991)
DOI:10.1128/JB.173.17.5315-5327.1991
Reference
PMID:1429451
  Authors
Yen KM, Karl MR
  Title
Identification of a new gene, tmoF, in the Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 174:7253-61 (1992)
DOI:10.1128/JB.174.22.7253-7261.1992
  Sequence
LinkDB

KEGG   ENZYME: 1.14.13.111
Entry
EC 1.14.13.111              Enzyme                                 
Name
methanesulfonate monooxygenase (NADH);
mesylate monooxygenase;
mesylate,reduced-FMN:oxygen oxidoreductase;
MsmABC;
methanesulfonic acid monooxygenase;
MSA monooxygenase;
MSAMO
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
methanesulfonate,NADH:oxygen oxidoreductase
Reaction(IUBMB)
methanesulfonate + NADH + H+ + O2 = formaldehyde + NAD+ + sulfite + H2O [RN:R09513]
Reaction(KEGG)
R09513
Substrate
methanesulfonate [CPD:C11145];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
formaldehyde [CPD:C00067];
NAD+ [CPD:C00003];
sulfite [CPD:C00094];
H2O [CPD:C00001]
Comment
A flavoprotein. Methanesulfonate is the simplest of the sulfonates and is a substrate for the growth of certain methylotrophic microorganisms. Compared with EC 1.14.14.5, alkanesulfonate monooxygenase, this enzyme has a restricted substrate range that includes only the short-chain aliphatic sulfonates (methanesulfonate to butanesulfonate) and excludes all larger molecules, such as arylsulfonates [1]. The enzyme from the bacterium Methylosulfonomonas methylovora is a multicomponent system comprising a hydroxylase, a reductase (MsmD) and a ferredoxin (MsmC). The hydroxylase has both large (MsmA) and small (MsmB) subunits, with each large subunit containing a Rieske-type [2Fe-2S] cluster. cf. EC 1.14.14.34, methanesulfonate monooxygenase (FMNH2).
History
EC 1.14.13.111 created 2009 as EC 1.14.14.6, transferred 2010 to EC 1.14.13.111, modified 2016
Pathway
ec00920  Sulfur metabolism
ec01100  Metabolic pathways
Orthology
K16968  methanesulfonate monooxygenase subunit alpha
K16969  methanesulfonate monooxygenase subunit beta
Genes
PQIKH389_17550 KH389_17555
CUHBJN34_09325 BJN34_09330 BJN34_22320
BCTGEM_5811 GEM_5812
BUEBRPE67_FCDS01800
PPKU875_05270 U875_05275
PPNODA70_22340 DA70_22345
PRBX636_04320 X636_04325
BPABPP0512 BPP0513
BBHBN112_2903 BN112_2904
BBRBB0517 BB0518
BBMBN115_0496 BN115_0497
BBXBBS798_0510 BBS798_0511
BTRMSAMEA390648701717(antA)
PUTPT7_2465 PT7_2466
PUDG9Q38_01125 G9Q38_01130
MPTMpe_A0919(msmS2) Mpe_A0920(msmB)
METPC1M51_13795
SPLBSFPGR_26190 SFPGR_26200
MNOMnod_2707 Mnod_2708 Mnod_8364 Mnod_8365
FILBN1229_v1_3548(msmB) BN1229_v1_3549(msmA)
FIYBN1229_v1_2374(msmA) BN1229_v1_2375(msmB)
APBSAR116_2102 SAR116_2103 SAR116_2109
 » show all
Reference
1  [PMID:10094704]
  Authors
de Marco P, Moradas-Ferreira P, Higgins TP, McDonald I, Kenna EM, Murrell JC
  Title
Molecular analysis of a novel methanesulfonic acid monooxygenase from the methylotroph Methylosulfonomonas methylovora.
  Journal
J Bacteriol 181:2244-51 (1999)
DOI:10.1128/JB.181.7.2244-2251.1999
  Sequence
Reference
2  [PMID:8932698]
  Authors
Higgins TP, Davey M, Trickett J, Kelly DP, Murrell JC
  Title
Metabolism of methanesulfonic acid involves a multicomponent monooxygenase enzyme.
  Journal
Microbiology 142 ( Pt 2):251-60 (1996)
DOI:10.1099/13500872-142-2-251
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.111
IUBMB Enzyme Nomenclature: 1.14.13.111
ExPASy - ENZYME nomenclature database: 1.14.13.111
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.13.111
BRENDA, the Enzyme Database: 1.14.13.111
LinkDB

KEGG   REACTION: R09513
Entry
R09513                      Reaction                               
Name
methanesulfonate,NADH:oxygen oxidoreductase
Definition
Methanesulfonic acid + NADH + H+ + Oxygen <=> Formaldehyde + NAD+ + Sulfite + H2O
Equation
Reaction class
RC00001  C00003_C00004
RC02556  C00067_C11145
Enzyme
Pathway
rn00920  Sulfur metabolism
rn01100  Metabolic pathways
Orthology
K15762  toluene monooxygenase system ferredoxin subunit
K15765  toluene monooxygenase electron transfer component [EC:1.18.1.3]
K16968  methanesulfonate monooxygenase subunit alpha [EC:1.14.13.111]
K16969  methanesulfonate monooxygenase subunit beta [EC:1.14.13.111]
Other DBs
RHEA: 26080
LinkDB

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