KEGG   ORTHOLOGY: K18365
Entry
K18365                      KO                                     
Symbol
bphI, xylK, nahM, tesG
Name
4-hydroxy-2-oxovalerate/4-hydroxy-2-oxohexanoate aldolase [EC:4.1.3.39 4.1.3.43]
Pathway
map00362  Benzoate degradation
map00621  Dioxin degradation
map00622  Xylene degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01220  Degradation of aromatic compounds
Module
M00569  Catechol meta-cleavage, catechol => acetyl-CoA / 4-methylcatechol => propanoyl-CoA
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K18365  bphI, xylK, nahM, tesG; 4-hydroxy-2-oxovalerate/4-hydroxy-2-oxohexanoate aldolase
   00622 Xylene degradation
    K18365  bphI, xylK, nahM, tesG; 4-hydroxy-2-oxovalerate/4-hydroxy-2-oxohexanoate aldolase
   00621 Dioxin degradation
    K18365  bphI, xylK, nahM, tesG; 4-hydroxy-2-oxovalerate/4-hydroxy-2-oxohexanoate aldolase
Enzymes [BR:ko01000]
 4. Lyases
  4.1  Carbon-carbon lyases
   4.1.3  Oxo-acid-lyases
    4.1.3.39  4-hydroxy-2-oxovalerate aldolase
     K18365  bphI, xylK, nahM, tesG; 4-hydroxy-2-oxovalerate/4-hydroxy-2-oxohexanoate aldolase
    4.1.3.43  4-hydroxy-2-oxohexanoate aldolase
     K18365  bphI, xylK, nahM, tesG; 4-hydroxy-2-oxovalerate/4-hydroxy-2-oxohexanoate aldolase
Dioxygenases [br01602.html]
 Dioxygenases in aromatic ring cleavage
  K18365
Other DBs
RN: R00750 R05298
COG: COG0119
GO: 0008701
Genes
PSD: DSC_09375
RGL: CS053_01770(dmpG)
RTH: LRK53_10290(dmpG)
RBD: ALSL_0877 ALSL_1082
VAF: D1115_22440(dmpG)
PRE: PCA10_13840
PFUW: KF707C_12190 KF707C_16050(salH) KF707C_16230(bphX3)
PPI: ND084(nahM)
PPUT: L483_14055
PPUN: PP4_27280(mhpE)
PPUU: PputUW4_01662(mhpE)
PKC: PKB_4237(nahM)
PSET: THL1_1435
PSIL: PMA3_11725
PKE: DLD99_14740(dmpG)
PUM: HGP31_20770(dmpG)
PPSH: G5J76_13480(dmpG)
PLAL: FXN65_06430(dmpG)
PAZE: KSS91_19285(dmpG)
PTAE: NCTC10697_02055(bphI)
AVN: Avin_30570(lapG)
AVL: AvCA_30570(lapG)
AVD: AvCA6_30570(lapG)
MARJ: MARI_05460(bphI)
ASJ: AsACE_CH00352(nahM)
ADV: DJ533_16960(dmpG)
ARJ: DOM24_01575(dmpG)
AWU: BEN71_02690(dmpG)
ACUM: C9E88_006015(dmpG)
ALW: FOB21_11710(dmpG)
ATN: FM020_13980(dmpG)
ACHI: CDG60_02965(dmpG)
ALJ: G8D99_13400(dmpG)
AWG: GFH30_01715(dmpG)
ASHA: G8E00_02255(dmpG)
ATG: J4G44_04390(dmpG)
AVB: RYU24_27150(mhpE)
ABOU: ACBO_27740(mhpE_2)
SPL: Spea_2116
SHL: Shal_2088
SWD: Swoo_1390
CBER: B5D82_11260(dmpG)
THT: E2K93_11210(dmpG)
PHA: PSHAa2143(mhpE)
PTN: PTRA_a2596(bphI)
PNG: PNIG_a2790(bphI)
SALH: HMF8227_02732(bphI)
TTU: TERTU_1283(dmpG)
SNAN: I6N98_10790(dmpG)
HALC: EY643_13285(dmpG)
AFUS: EYZ66_02685(dmpG) EYZ66_12975(dmpG)
CYQ: Q91_0510 Q91_1491(lapG)
CZA: CYCME_0969(lapG) CYCME_2101(dmpG)
CYY: CPC19_05395(dmpG) CPC19_10505(dmpG)
BLEP: AL038_01290(dmpG)
THIS: HZT40_10615(dmpG)
AEH: Mlg_2462
KAK: Kalk_07865(dmpG)
MPRI: MP3633_3149(mhpE)
MGEO: CFI10_00435(dmpG) CFI10_04970(dmpG) CFI10_06050(dmpG)
MSEC: LN244_04515(dmpG) LN244_04765(dmpG) LN244_11320(dmpG)
NCU: F0U83_03055(dmpG)
NJP: NEJAP_0673(mhpE)
NIK: F5I99_02020(dmpG) F5I99_10095(dmpG) F5I99_10510(dmpG)
ASIM: FE240_03470(dmpG)
SOK: D0B54_06190(dmpG)
SINI: GT972_14660(dmpG)
FCE: JN531_005840(dmpG)
TBN: TBH_C0930
GPB: HDN1F_27070(tesG)
NZO: SAMEA4504057_0444(bphI)
NCI: NCTC10296_00917(bphI)
NWD: H3L96_04775(dmpG)
NDU: LVJ88_00580(dmpG)
PTES: JQU52_04850(dmpG)
USU: LVJ78_03650(dmpG)
RPI: Rpic_4617
REH: H16_B0595(bpHI)
CNC: CNE_2c05400(bpHI)
RME: Rmet_1319(bphI)
CTI: RALTA_B0399(mhpE)
COX: E0W60_08015(dmpG)
BCJ: BCAM1615(bphI)
BCEN: DM39_3381(dmpG) DM39_4832(dmpG)
BCEO: I35_5475(bphI)
BCED: DM42_4991(dmpG) DM42_6343(dmpG)
BMEC: WJ16_26800
BANN: JFN94_19135(dmpG) JFN94_25660(dmpG)
BURK: DM992_32615(dmpG)
BXE: Bxe_C1187(bphI)
BXB: DR64_8618(dmpG)
BFN: OI25_4591(dmpG)
PARB: CJU94_37880(dmpG) CJU94_38125(dmpG)
PMEG: FNZ07_21155(dmpG)
CABA: SBC2_49000(bphI)
LIMN: HKT17_03830(dmpG)
AXY: AXYL_04307(dmpG)
RFR: Rfer_0454
PNA: Pnap_4142
AJS: Ajs_0224
AMON: H9L24_14175(dmpG)
VEI: Veis_2781
VBO: CKY39_28495(dmpG)
CTES: O987_07730
COF: FOZ74_08315(dmpG)
HPSE: HPF_01850(bphI)
HYN: F9K07_02775(dmpG) F9K07_11450(dmpG) F9K07_31030(dmpG)
HTN: KI616_01945(dmpG)
DIH: G7047_09695(dmpG)
SIMP: C6571_13625(dmpG)
MELA: C6568_07850(dmpG)
MJE: LVC68_01015(dmpG)
STHM: IS481_10550(dmpG)
METP: C1M51_06440(dmpG) C1M51_16320(dmpG)
JAJ: EKL02_01800(dmpG) EKL02_13150(dmpG)
LCH: Lcho_3340
SNN: EWH46_19310(dmpG)
IDC: LRM40_19585(dmpG)
ABRE: pbN1_20580(mhpE)
DEY: HYN24_14110(dmpG) HYN24_14200(dmpG)
QAU: KI612_15145(dmpG)
FLE: KI610_08595(dmpG) KI610_13435(dmpG) KI610_15450(dmpG)
AZO: azo1857(lapG) azo1972(mhpE) azo2431(nahM)
ACOM: CEW83_03610(dmpG)
AZD: CDA09_09965(dmpG) CDA09_10335(dmpG) CDA09_10460(dmpG) CDA09_21430(dmpG)
AZR: CJ010_12270(dmpG) CJ010_12835(dmpG) CJ010_15205(dmpG)
THK: CCZ27_04605(dmpG) CCZ27_10750(dmpG)
AZQ: G3580_00550(dmpG) G3580_08280(dmpG)
ACIB: ACBT_0259(dmpG)
AFC: AFAEC_1844(dmpG)
AAQI: AAQM_0982(dmpG)
ACLO: ACLO_a0139(dmpG)
ADZ: ADFLV_2551(dmpG)
ALK: ALEK_0383(dmpG)
AANA: AANAER_1260(dmpG)
ARC: ABLL_2442
SHZ: shn_33310
CID: P73_1382
TEC: AKL02_008270(dmpG)
SGI: SGRAN_1579(mhpE)
SJP: SJA_C1-11910(mhpE_xylK)
SINB: SIDU_08310
SHYD: CJD35_06515(dmpG)
SBAR: H5V43_20635(dmpG)
SFLA: SPHFLASMR4Y_02366(bphI)
TFV: IDJ81_04960(dmpG)
TXI: TH3_21418
HTL: HPTL_0047
GGH: GHH_c14840(pheE)
AAD: TC41_0802(mhpE)
BTS: Btus_0969
KYR: CVV65_10345(dmpG) CVV65_11290(dmpG)
DHD: Dhaf_1245
TOC: Toce_0378
 » show all
Reference
  Authors
Horinouchi M, Hayashi T, Koshino H, Kurita T, Kudo T
  Title
Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441.
  Journal
Appl Environ Microbiol 71:5275-81 (2005)
DOI:10.1128/AEM.71.9.5275-5281.2005
  Sequence
LinkDB

KEGG   ENZYME: 4.1.3.43
Entry
EC 4.1.3.43                 Enzyme                                 
Name
4-hydroxy-2-oxohexanoate aldolase;
BphI
Class
Lyases;
Carbon-carbon lyases;
Oxo-acid-lyases
Sysname
(S)-4-hydroxy-2-oxohexanoate pyruvate-lyase (propanal-forming)
Reaction(IUBMB)
(S)-4-hydroxy-2-oxohexanoate = propanal + pyruvate
Reaction(KEGG)
(other) R05298
Substrate
(S)-4-hydroxy-2-oxohexanoate
Product
propanal [CPD:C00479];
pyruvate [CPD:C00022]
Comment
Requires Mn2+ for maximal activity [1,2]. The enzymes from the bacteria Burkholderia xenovorans and Thermus thermophilus also perform the reaction of EC 4.1.3.39, 4-hydroxy-2-oxovalerate aldolase [1,2,6]. The enzyme forms a bifunctional complex with EC 1.2.1.87, propanal dehydrogenase (CoA-propanoylating), with a tight channel connecting the two subunits [3,4,6].
History
EC 4.1.3.43 created 2013
Pathway
ec00622  Xylene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K18365  4-hydroxy-2-oxovalerate/4-hydroxy-2-oxohexanoate aldolase
Genes
PSDDSC_09375
RGLCS053_01770(dmpG)
RTHLRK53_10290(dmpG)
RBDALSL_0877 ALSL_1082
VAFD1115_22440(dmpG)
PREPCA10_13840
PFUWKF707C_12190 KF707C_16050(salH) KF707C_16230(bphX3)
PPIND084(nahM)
PPUTL483_14055
PPUNPP4_27280(mhpE)
PVRPverR02_11585 PverR02_14710
PSCA458_06510
PSTUUIB01_22750
PBMCL52_14720
PPUUPputUW4_01662(mhpE)
PKCPKB_4237(nahM)
PFZAV641_10525
PALKPSAKL28_05980
PPSYAOC04_07825
PFKPFAS1_28765
PSETTHL1_1435
PSILPMA3_11725
PKEDLD99_14740(dmpG)
PALLUYA_06970 UYA_07050
PUMHGP31_20770(dmpG)
PPSHG5J76_13480(dmpG)
PLALFXN65_06430(dmpG)
PAZEKSS91_19285(dmpG)
PPHABVH74_17970
PTAENCTC10697_02055(bphI)
AVNAvin_30570(lapG)
AVLAvCA_30570(lapG)
AVDAvCA6_30570(lapG)
MARJMARI_05460(bphI)
ALCOTEC02_08820
AEIAOY20_01975
ASJAsACE_CH00352(nahM)
ADVDJ533_16960(dmpG)
ARJDOM24_01575(dmpG)
AWUBEN71_02690(dmpG)
ACUMC9E88_006015(dmpG)
ALWFOB21_11710(dmpG)
ATNFM020_13980(dmpG)
ACHICDG60_02965(dmpG)
ALJG8D99_13400(dmpG)
AWGGFH30_01715(dmpG)
ASHAG8E00_02255(dmpG)
ATGJ4G44_04390(dmpG)
AVBRYU24_27150(mhpE)
ABOUACBO_27740(mhpE_2)
SPLSpea_2116
SHLShal_2088
SWDSwoo_1390
COLWA3Q33_18585
CBERB5D82_11260(dmpG)
THTE2K93_11210(dmpG)
PHAPSHAa2143(mhpE)
PTNPTRA_a2596(bphI)
PNGPNIG_a2790(bphI)
LALAT746_01290
SALHHMF8227_02732(bphI)
TTUTERTU_1283(dmpG)
SPOIIMCC21906_00395 IMCC21906_02714
SNANI6N98_10790(dmpG)
ZALAZF00_03870 AZF00_11420 AZF00_17810
HJABST95_09515
HALCEY643_13285(dmpG)
AFUSEYZ66_02685(dmpG) EYZ66_12975(dmpG)
CYQQ91_0510 Q91_1491(lapG)
CZACYCME_0969(lapG) CYCME_2101(dmpG)
CYYCPC19_05395(dmpG) CPC19_10505(dmpG)
BLEPAL038_01290(dmpG)
THISHZT40_10615(dmpG)
AEHMlg_2462
KAKKalk_07865(dmpG)
MMWMmwyl1_3801
MPRIMP3633_3149(mhpE)
MGEOCFI10_00435(dmpG) CFI10_04970(dmpG) CFI10_06050(dmpG)
MSECLN244_04515(dmpG) LN244_04765(dmpG) LN244_11320(dmpG)
NCUF0U83_03055(dmpG)
NJPNEJAP_0673(mhpE)
NIKF5I99_02020(dmpG) F5I99_10095(dmpG) F5I99_10510(dmpG)
AJPAMJAP_2526 AMJAP_2997
ASIMFE240_03470(dmpG)
ZDFAN401_14615
SOKD0B54_06190(dmpG)
SINIGT972_14660(dmpG)
FCEJN531_005840(dmpG)
GBIPG2T_09675
TBNTBH_C0930
GPBHDN1F_27070(tesG)
NZOSAMEA4504057_0444(bphI)
NCINCTC10296_00917(bphI)
NWDH3L96_04775(dmpG)
NDULVJ88_00580(dmpG)
VFFVITFI_CDS1376
PTESJQU52_04850(dmpG)
USULVJ78_03650(dmpG)
AQLBXU06_16260 BXU06_16885 BXU06_17065
RPIRpic_4617
RPFRpic12D_3541
RINACS15_4356
REHH16_B0595(bpHI)
CNCCNE_2c05400(bpHI)
CUHBJN34_24650
RMERmet_1319(bphI)
CTIRALTA_B0399(mhpE)
CBWRR42_s2315
COXE0W60_08015(dmpG)
BVIBcep1808_7605
BURBcep18194_B1457 Bcep18194_B2959
BCJBCAM1615(bphI)
BCENDM39_3381(dmpG) DM39_4832(dmpG)
BCEOI35_5475(bphI)
BCTGEM_4109 GEM_5401
BCEDDM42_4991(dmpG) DM42_6343(dmpG)
BCEPAPZ15_31145
BPYRABD05_27810
BCONNL30_04130 NL30_24590
BDFWI26_15995 WI26_21155
BTEIWS51_00570 WS51_05705
BPSLWS57_06035 WS57_12155
BMECWJ16_26800
BSTLBBJ41_10520 BBJ41_21350
BANNJFN94_19135(dmpG) JFN94_25660(dmpG)
BUQAC233_11185
BURKDM992_32615(dmpG)
BXEBxe_C1187(bphI)
BXBDR64_8618(dmpG)
BFNOI25_4591(dmpG)
PARBCJU94_37880(dmpG) CJU94_38125(dmpG)
PCAFDSC91_003289
PMEGFNZ07_21155(dmpG)
PPKU875_01890
PRBX636_07580
CABASBC2_49000(bphI)
LIMNHKT17_03830(dmpG)
AXYAXYL_04307(dmpG)
CDNBN940_14431
RFRRfer_0454
PNAPnap_4142
AJSAjs_0224
ACIDCBP33_01115
ACIPCBP36_01210
ACINCBP34_01120
ACISCBP35_17740
AMONH9L24_14175(dmpG)
VEIVeis_2781
VBOCKY39_28495(dmpG)
CTTCtCNB1_1351 CtCNB1_3147
CTESO987_07730
COFFOZ74_08315(dmpG)
ADNAlide_0339
ADKAlide2_0284
HYLLPB072_18200
HPSEHPF_01850(bphI)
HYNF9K07_02775(dmpG) F9K07_11450(dmpG) F9K07_31030(dmpG)
HTNKI616_01945(dmpG)
DIHG7047_09695(dmpG)
SIMPC6571_13625(dmpG)
MELAC6568_07850(dmpG)
MJELVC68_01015(dmpG)
STHMIS481_10550(dmpG)
MPTMpe_A2266 Mpe_A3321
METPC1M51_06440(dmpG) C1M51_16320(dmpG)
JAJEKL02_01800(dmpG) EKL02_13150(dmpG)
MASWAM586_18660
LCHLcho_3340
SNNEWH46_19310(dmpG)
IDCLRM40_19585(dmpG)
RBUPG1C_14090
ABREpbN1_20580(mhpE)
DARDaro_0907 Daro_1356 Daro_3782 Daro_3808
DEYHYN24_14110(dmpG) HYN24_14200(dmpG)
QAUKI612_15145(dmpG)
FLEKI610_08595(dmpG) KI610_13435(dmpG) KI610_15450(dmpG)
AZOazo1857(lapG) azo1972(mhpE) azo2431(nahM)
AOAdqs_2010 dqs_2127 dqs_2575
AZIAzCIB_1509 AzCIB_4409
ACOMCEW83_03610(dmpG)
AZDCDA09_09965(dmpG) CDA09_10335(dmpG) CDA09_10460(dmpG) CDA09_21430(dmpG)
AZRCJ010_12270(dmpG) CJ010_12835(dmpG) CJ010_15205(dmpG)
TMZTmz1t_2079 Tmz1t_3105
THUAC731_001820 AC731_008905
THKCCZ27_04605(dmpG) CCZ27_10750(dmpG)
AZQG3580_00550(dmpG) G3580_08280(dmpG)
ACIBACBT_0259(dmpG)
AFCAFAEC_1844(dmpG)
AAQIAAQM_0982(dmpG)
ACLOACLO_a0139(dmpG)
ADZADFLV_2551(dmpG)
ALKALEK_0383(dmpG)
AANAAANAER_1260(dmpG)
ARCABLL_2442
SHZshn_33310
CIDP73_1382
DAAAKL17_3p0139
TECAKL02_008270(dmpG)
PGVSL003B_p0059
NPPPP1Y_AT32722
SGISGRAN_1579(mhpE)
SJPSJA_C1-11910(mhpE_xylK)
SINBSIDU_08310
SYBTZ53_09030 TZ53_23475
SHYDCJD35_06515(dmpG)
SBARH5V43_20635(dmpG)
SFLASPHFLASMR4Y_02366(bphI)
SPHJBSL82_10435
TFVIDJ81_04960(dmpG)
TXITH3_21418
HTLHPTL_0047
GYCGYMC61_2298
GYAGYMC52_1426
GCTGC56T3_2055
GGHGHH_c14840(pheE)
GJFM493_17920
PNPIJ22_48590
AADTC41_0802(mhpE)
BTSBtus_0969
KYRCVV65_10345(dmpG) CVV65_11290(dmpG)
LACYA4V08_07730
DHDDhaf_1245
DCADesca_1927
MTHOMOTHE_c17890
MTHZMOTHA_c18720
TOCToce_0378
STEDSPTER_03000 SPTER_36960(bphI)
SSMSpirs_4175
NAJB1756_14020 B1756_15945
 » show all
Reference
1  [PMID:19476337]
  Authors
Baker P, Pan D, Carere J, Rossi A, Wang W, Seah SY
  Title
Characterization of an aldolase-dehydrogenase complex that exhibits substrate channeling in the polychlorinated biphenyls degradation pathway.
  Journal
Biochemistry 48:6551-8 (2009)
DOI:10.1021/bi9006644
  Sequence
[bxe:Bxe_C1187]
Reference
2  [PMID:20364820]
  Authors
Wang W, Baker P, Seah SY
  Title
Comparison of two metal-dependent pyruvate aldolases related by convergent evolution: substrate specificity, kinetic mechanism, and substrate channeling.
  Journal
Biochemistry 49:3774-82 (2010)
DOI:10.1021/bi100251u
  Sequence
[bxe:Bxe_C1187]
Reference
3  [PMID:21425833]
  Authors
Baker P, Carere J, Seah SY
  Title
Probing the molecular basis of substrate specificity, stereospecificity, and catalysis in the class II pyruvate aldolase, BphI.
  Journal
Biochemistry 50:3559-69 (2011)
DOI:10.1021/bi101947g
  Sequence
[bxe:Bxe_C1187]
Reference
4  [PMID:21838275]
  Authors
Carere J, Baker P, Seah SY
  Title
Investigating the molecular determinants for substrate channeling in BphI-BphJ, an aldolase-dehydrogenase complex from the polychlorinated biphenyls degradation  pathway.
  Journal
Biochemistry 50:8407-16 (2011)
DOI:10.1021/bi200960j
  Sequence
[bxe:Bxe_C1187]
Reference
5  [PMID:22081904]
  Authors
Baker P, Seah SY
  Title
Rational design of stereoselectivity in the class II pyruvate aldolase BphI.
  Journal
J Am Chem Soc 134:507-13 (2012)
DOI:10.1021/ja208754r
  Sequence
[bxe:Bxe_C1187]
Reference
6  [PMID:22316175]
  Authors
Baker P, Hillis C, Carere J, Seah SY
  Title
Protein-protein interactions and substrate channeling in orthologous and chimeric aldolase-dehydrogenase complexes.
  Journal
Biochemistry 51:1942-52 (2012)
DOI:10.1021/bi201832a
  Sequence
[ttj:TTHB246]
Other DBs
ExplorEnz - The Enzyme Database: 4.1.3.43
IUBMB Enzyme Nomenclature: 4.1.3.43
ExPASy - ENZYME nomenclature database: 4.1.3.43
BRENDA, the Enzyme Database: 4.1.3.43
LinkDB

KEGG   REACTION: R05298
Entry
R05298                      Reaction                               
Name
4-hydroxy-2-oxohexanoate pyruvate-lyase (propanal-forming)
Definition
Propanal + Pyruvate <=> 4-Hydroxy-2-oxohexanoic acid
Equation
Reaction class
RC00307  C00022_C06762
RC00572  C00479_C06762
Enzyme
Pathway
rn00622  Xylene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01220  Degradation of aromatic compounds
Module
M00569  Catechol meta-cleavage, catechol => acetyl-CoA / 4-methylcatechol => propanoyl-CoA
Orthology
K18365  4-hydroxy-2-oxovalerate/4-hydroxy-2-oxohexanoate aldolase [EC:4.1.3.39 4.1.3.43]
Other DBs
RHEA: 36006
LinkDB

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