KEGG   ORTHOLOGY: K18565
Entry
K18565                      KO                                     
Symbol
thnG, cmmG
Name
2-oxoglutarate-dependent dioxygenase [EC:1.14.20.-]
Pathway
map00332  Carbapenem biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R10740  2,3-dihydrothienamycin,2-oxoglutarate:oxygen oxidoreductase (dehydrating)
R10741  epithienamycin-F,2-oxoglutarate:oxygen oxidoreductase (dehydrating)
R10742  epithienamycin-E,2-oxoglutarate:oxygen oxidoreductase (sulfoxidation)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00332 Carbapenem biosynthesis
    K18565  thnG, cmmG; 2-oxoglutarate-dependent dioxygenase
Genes
SFA: Sfla_0162
STRP: F750_6859
SFIY: F0344_10125
SCT: SCAT_p0826(thnG)
SCY: SCATT_p09090
Reference
PMID:20017478
  Authors
Bodner MJ, Phelan RM, Freeman MF, Li R, Townsend CA
  Title
Non-heme iron oxygenases generate natural structural diversity in carbapenem antibiotics.
  Journal
J Am Chem Soc 132:12-3 (2010)
DOI:10.1021/ja907320n
Reference
PMID:21263049
  Authors
Rodriguez M, Nunez LE, Brana AF, Mendez C, Salas JA, Blanco G
  Title
Mutational analysis of the thienamycin biosynthetic gene cluster from Streptomyces cattleya.
  Journal
Antimicrob Agents Chemother 55:1638-49 (2011)
DOI:10.1128/AAC.01366-10
  Sequence
[sct:SCAT_p0826]
Reference
PMID:24420617
  Authors
Li R, Lloyd EP, Moshos KA, Townsend CA
  Title
Identification and characterization of the carbapenem MM 4550 and its gene cluster in Streptomyces argenteolus ATCC 11009.
  Journal
Chembiochem 15:320-31 (2014)
DOI:10.1002/cbic.201300319
LinkDB

KEGG   REACTION: R10741
Entry
R10741                      Reaction                               
Name
epithienamycin-F,2-oxoglutarate:oxygen oxidoreductase (dehydrating)
Definition
Epithienamycin F + 2-Oxoglutarate + Oxygen <=> Epithienamycin E + Succinate + CO2 + H2O
Equation
C17399 + C00026 + C00007 <=> C11755 + C00042 + C00011 + C00001
Comment
proposed pathway
not yet characterized sufficiently
Reaction class
RC03268  C11755_C17399
Enzyme
1.14.20.-
Pathway
rn00332  Carbapenem biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.20  With 2-oxoglutarate as one donor, and the other dehydrogenated
    1.14.20.-
     R10741  Epithienamycin F + 2-Oxoglutarate + Oxygen <=> Epithienamycin E + Succinate + CO2 + H2O
Orthology
K18565  2-oxoglutarate-dependent dioxygenase [EC:1.14.20.-]
Reference
1  [PMID:24420617]
  Authors
Li R, Lloyd EP, Moshos KA, Townsend CA
  Title
Identification and characterization of the carbapenem MM 4550 and its gene cluster in Streptomyces argenteolus ATCC 11009.
  Journal
Chembiochem 15:320-31 (2014)
DOI:10.1002/cbic.201300319
LinkDB

DBGET integrated database retrieval system