KEGG ORTHOLOGY: K19104

ORTHOLOGY: K19104

Entry

K19104                      KO

Symbol

nat

Name

isonocardicin synthase [EC:2.5.1.38]

Pathway

map00261

Monobactam biosynthesis

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Module

M00736

Nocardicin A biosynthesis, L-pHPG + arginine + serine => nocardicin A

Reaction

R03072

S-adenosyl-L-methionine:nocardicin-E 3-amino-3-carboxypropyltransferase

R10881

S-adenosyl-L-methionine:nocardicin-G 3-amino-3-carboxypropyltransferase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00261 Monobactam biosynthesis
    K19104  nat; isonocardicin synthase
09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K19104  nat; isonocardicin synthase
Enzymes [BR:ko01000]
2. Transferases
  2.5  Transferring alkyl or aryl groups, other than methyl groups
   2.5.1  Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
    2.5.1.38  isonocardicin synthase
     K19104  nat; isonocardicin synthase
Polyketide biosynthesis proteins [BR:ko01008]
Nonribosomal peptide tailoring proteins
  Others
   K19104  nat; isonocardicin synthase

Other DBs

GO:

0050007

Genes

PTRT:

HU722_0017545

PORY:

EJA05_18815

PWY:	HU734_009855

AMI:

ACTI:

ACAD:

ACTA:

ACAU:

AG:	AAD46393(nat)

Reference

PMID:9804844

Authors

Reeve AM, Breazeale SD, Townsend CA

Title

Purification, characterization, and cloning of an S-adenosylmethionine-dependent 3-amino-3-carboxypropyltransferase in nocardicin biosynthesis.

Journal

J Biol Chem 273:30695-703 (1998)
DOI:10.1074/jbc.273.46.30695

Reference

PMID:15271351

Authors

Gunsior M, Breazeale SD, Lind AJ, Ravel J, Janc JW, Townsend CA

Title

The biosynthetic gene cluster for a monocyclic beta-lactam antibiotic, nocardicin A.

Journal

Chem Biol 11:927-38 (2004)
DOI:10.1016/j.chembiol.2004.04.012

Sequence

[ag:AAD46393]

LinkDB

ENZYME: 2.5.1.38

Entry

EC 2.5.1.38                 Enzyme

Name

isonocardicin synthase;
nocardicin aminocarboxypropyltransferase;
S-adenosyl-L-methionine:nocardicin-E 3-amino-3-carboxypropyltransferase

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

S-adenosyl-L-methionine:nocardicin-G 3-amino-3-carboxypropyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + nocardicin G = S-methyl-5'-thioadenosine + isonocardicin C [RN:R10881]

Reaction(KEGG)

R10881;
(other) R03072

Substrate

S-adenosyl-L-methionine [CPD:C00019];
nocardicin G [CPD:C17355]

Product

S-methyl-5'-thioadenosine [CPD:C00170];
isonocardicin C [CPD:C17352]

Comment

The enzyme, characterized from the bacterium Nocardia uniformis, is involved in the biosynthesis of the beta-lactam antibiotic nocardicin A. The enzyme can act on nocardicin E, F, and G, producing isonocardicin A, B, and C, respectively. However, the in vivo substrate is believed to be nocardicin G [3].

History

EC 2.5.1.38 created 1992, modified 2016

Pathway

ec00261

Monobactam biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K19104

isonocardicin synthase

Genes

PTRT:

HU722_0017545

PORY:

EJA05_18815

PWY:	HU734_009855

AMI:

Amir_4587

ACTI:

UA75_15395

ACAD:

UA74_14825

ACTA:

C1701_23395

ACAU:

ACOJVX_14450

Reference

Authors

Wilson BA, Bantia S, Salituro GM, Reeve AM, Townsend CA.

Title

Cell-free biosynthesis of nocardicin A from nocardicin E and S-adenosylmethionine.

Journal

J Am Chem Soc 110:8238-8239 (1988)

Reference

2 [PMID:9804844]

Authors

Reeve AM, Breazeale SD, Townsend CA

Title

Purification, characterization, and cloning of an S-adenosylmethionine-dependent 3-amino-3-carboxypropyltransferase in nocardicin biosynthesis.

Journal

J Biol Chem 273:30695-703 (1998)
DOI:10.1074/jbc.273.46.30695

Reference

3 [PMID:15629944]

Authors

Kelly WL, Townsend CA

Title

Mutational analysis of nocK and nocL in the nocardicin a producer Nocardia uniformis.

Journal

J Bacteriol 187:739-46 (2005)
DOI:10.1128/JB.187.2.739-746.2005

Other DBs

ExplorEnz - The Enzyme Database:

2.5.1.38

IUBMB Enzyme Nomenclature:

2.5.1.38

ExPASy - ENZYME nomenclature database:

2.5.1.38

BRENDA, the Enzyme Database:

2.5.1.38

CAS:	118246-74-5

LinkDB

REACTION: R10881

Entry

R10881                      Reaction

Name

S-adenosyl-L-methionine:nocardicin-G 3-amino-3-carboxypropyltransferase

Definition

S-Adenosyl-L-methionine + Nocardicin G <=> 5'-Methylthioadenosine + Isonocardicin C

Equation

C00019 + C17355 <=> C00170 + C17352

Reaction class

RC00021

C00019_C00170

RC01372

C17352_C17355

Enzyme

2.5.1.38

Pathway

rn00261

Monobactam biosynthesis

rn01100

Metabolic pathways

rn01110

Biosynthesis of secondary metabolites

Module

M00736

Nocardicin A biosynthesis, L-pHPG + arginine + serine => nocardicin A

Brite

Enzymatic reactions [BR:br08201]
2. Transferase reactions
  2.5  Transferring alkyl or aryl groups, other than methyl groups
   2.5.1  Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
    2.5.1.38
     R10881  S-Adenosyl-L-methionine + Nocardicin G <=> 5'-Methylthioadenosine + Isonocardicin C

Orthology

K19104

isonocardicin synthase [EC:2.5.1.38]

Other DBs

RHEA:

49711

LinkDB

DBGET integrated database retrieval system