KEGG   ORTHOLOGY: K19105
Entry
K19105                      KO                                     
Symbol
nocJ
Name
nocardicin-A epimerase [EC:5.1.1.14]
Pathway
map00261  Monobactam biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00736  Nocardicin A biosynthesis, L-pHPG + arginine + serine => nocardicin A
Reaction
R03073  nocardicin-A epimerase
R10883  nocardicin-C epimerase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00261 Monobactam biosynthesis
    K19105  nocJ; nocardicin-A epimerase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K19105  nocJ; nocardicin-A epimerase
Enzymes [BR:ko01000]
 5. Isomerases
  5.1  Racemases and epimerases
   5.1.1  Acting on amino acids and derivatives
    5.1.1.14  nocardicin A epimerase
     K19105  nocJ; nocardicin-A epimerase
Polyketide biosynthesis proteins [BR:ko01008]
 Nonribosomal peptide tailoring proteins
  Others
   K19105  nocJ; nocardicin-A epimerase
Other DBs
GO: 0050143
Genes
AMI: Amir_4594
ACTI: UA75_15360
ACAD: UA74_14790
ACTA: C1701_23430
ACAU: ACOJVX_14415
AG: AAT09799(nocJ)
Reference
PMID:15252031
  Authors
Kelly WL, Townsend CA
  Title
Mutational analysis and characterization of nocardicin C-9' epimerase.
  Journal
J Biol Chem 279:38220-7 (2004)
DOI:10.1074/jbc.M405450200
Reference
PMID:15271351
  Authors
Gunsior M, Breazeale SD, Lind AJ, Ravel J, Janc JW, Townsend CA
  Title
The biosynthetic gene cluster for a monocyclic beta-lactam antibiotic, nocardicin A.
  Journal
Chem Biol 11:927-38 (2004)
DOI:10.1016/j.chembiol.2004.04.012
  Sequence
[ag:AAT09799]
LinkDB

KEGG   ENZYME: 5.1.1.14
Entry
EC 5.1.1.14                 Enzyme                                 
Name
nocardicin A epimerase;
isonocardicin A epimerase;
nocJ (gene name)
Class
Isomerases;
Racemases and epimerases;
Acting on amino acids and derivatives
Sysname
nocardicin-C epimerase
Reaction(IUBMB)
(1) isonocardicin C = nocardicin C [RN:R10883];
(2) isonocardicin A = nocardicin A [RN:R03073]
Reaction(KEGG)
R03073 R10883
Substrate
isonocardicin C [CPD:C17352];
isonocardicin A [CPD:C00927]
Product
nocardicin C [CPD:C17351];
nocardicin A [CPD:C01941]
Comment
Requires pyridoxal 5'-phosphate. The enzyme, characterized from the bacterium Nocardia uniformis, is involved in the biosynthesis of the monolactam antibiotic nocardicin A. It catalyses the epimerization of the amino group at position 9' from (S)- configuration to (R)-. The enzyme can act on both isonocardicin A and isonocardicin C, but the in vivo substrate appears to be the latter [3].
History
EC 5.1.1.14 created 1992, modified 2016
Pathway
ec00261  Monobactam biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K19105  nocardicin-A epimerase
Genes
AMI: Amir_4594
ACTI: UA75_15360
ACAD: UA74_14790
ACTA: C1701_23430
ACAU: ACOJVX_14415
Reference
1
  Authors
Wilson BA, Bantia S, Salituro GM, Reeve AM, Townsend CA.
  Title
Cell-free biosynthesis of nocardicin A from nocardicin E and S-adenosylmethionine.
  Journal
J Am Chem Soc 110:8238-8239 (1988)
Reference
2  [PMID:15252031]
  Authors
Kelly WL, Townsend CA
  Title
Mutational analysis and characterization of nocardicin C-9' epimerase.
  Journal
J Biol Chem 279:38220-7 (2004)
DOI:10.1074/jbc.M405450200
Reference
3  [PMID:15629944]
  Authors
Kelly WL, Townsend CA
  Title
Mutational analysis of nocK and nocL in the nocardicin a producer Nocardia uniformis.
  Journal
J Bacteriol 187:739-46 (2005)
DOI:10.1128/JB.187.2.739-746.2005
Other DBs
ExplorEnz - The Enzyme Database: 5.1.1.14
IUBMB Enzyme Nomenclature: 5.1.1.14
ExPASy - ENZYME nomenclature database: 5.1.1.14
BRENDA, the Enzyme Database: 5.1.1.14
CAS: 118246-75-6
LinkDB

KEGG   REACTION: R03073
Entry
R03073                      Reaction                               
Name
nocardicin-A epimerase;
isonocardicin-A epimerase
Definition
Isonocardicin A <=> Nocardicin A
Equation
C00927 <=> C01941
Reaction class
RC00302  C00927_C01941
Enzyme
5.1.1.14
Pathway
rn00261  Monobactam biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00736  Nocardicin A biosynthesis, L-pHPG + arginine + serine => nocardicin A
Brite
Enzymatic reactions [BR:br08201]
 5. Isomerase reactions
  5.1  Racemases and epimerases
   5.1.1  Acting on amino acids and derivatives
    5.1.1.14
     R03073  Isonocardicin A <=> Nocardicin A
Orthology
K19105  nocardicin-A epimerase [EC:5.1.1.14]
Other DBs
RHEA: 22795
LinkDB

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