KEGG   ORTHOLOGY: K19290
Entry
K19290                      KO                                     
Symbol
alg8
Name
mannuronan synthase [EC:2.4.1.33]
Pathway
map00051  Fructose and mannose metabolism
map01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00051 Fructose and mannose metabolism
    K19290  alg8; mannuronan synthase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01003 Glycosyltransferases
    K19290  alg8; mannuronan synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.33  mannuronan synthase
     K19290  alg8; mannuronan synthase
Glycosyltransferases [BR:ko01003]
 Polysaccharide
  Bacterial polysaccharide (excluding LPS)
   K19290  alg8; mannuronan synthase
Other DBs
RN: R08692
COG: COG1215
TC: 4.D.1.1.7 4.D.1.1.9
CAZy: GT2
Genes
PAE: PA3541(alg8)
PAEV: N297_3664
PAEI: N296_3664
PAU: PA14_18565(alg8)
PAP: PSPA7_1604
PAG: PLES_14921(alg8)
PAF: PAM18_1445(alg8)
PNC: NCGM2_4666(alg8)
PAEB: NCGM1900_2743(alg8)
PDK: PADK2_06660
PAEP: PA1S_07715
PAEM: U769_07190
PAEL: T223_07430
PAEG: AI22_26190
PAEC: M802_3661
PAEO: M801_3529
PMK: MDS_1081
PRE: PCA10_11870(alg8) PCA10_38220(alg8)
PPU: PP_1287(alg8)
PPF: Pput_4438
PPT: PPS_4404
PPI: YSA_03094
PPX: T1E_3167
PPUH: B479_22155
PPUT: L483_27240
PPUN: PP4_07990(alg8)
PPUD: DW66_4795
PMON: X969_21690
PMOT: X970_21325
PSB: Psyr_1062
PSYR: N018_20360
PVD: CFBP1590__4278(alg8)
PCAB: JGS08_19530(alg8)
PFL: PFL_1023
PPRC: PFLCHA0_c10430(alg2)
PPRO: PPC_1061
PFE: PSF113_4753(alg8)
PFS: PFLU_0989
PFB: VO64_4070
PMAN: OU5_2448
PFW: PF1751_v1c09470(alg8)
PEN: PSEEN4536(alg8)
PPUU: PputUW4_04472(alg8)
PKC: PKB_0967(alg8)
PSES: PSCI_0722
PSEM: TO66_05205
PSEC: CCOS191_0911(alg8a) CCOS191_2028(alg8b)
PSOS: POS17_1029
PANR: A7J50_0991
PSIL: PMA3_24590
POJ: PtoMrB4_25980(alg8_1) PtoMrB4_46200(alg8_2)
PKH: JLK41_05215(alg8)
PATA: JWU58_22755(alg8)
PDW: BV82_3322 BV82_4691(alg8)
PZE: HU754_006265(alg8)
PMOE: HV782_005845(alg8)
PSEP: C4K39_1040
PVW: HU752_027435(alg8)
PTRT: HU722_0006160(alg8)
PSAM: HU731_021000(alg8)
PTZ: HU718_005905(alg8)
PSHH: HU773_022840(alg8)
PHV: HU739_012715(alg8)
PASG: KSS96_05690(alg8)
PAZE: KSS91_23850(alg8)
PALV: KSS97_06290(alg8)
PWZ: J7655_04685(alg8)
PTW: TUM18999_50130(alg8)
PZA: HU749_024145(alg8)
PIE: HU724_005665(alg8)
AVN: Avin_10960(alg8)
AVL: AvCA_10960(alg8)
AVD: AvCA6_10960(alg8)
ACX: Achr_31190(alg8)
PSPO: PSPO_a3133
ABO: ABO_0385(alg8)
TAU: Tola_1408
ENM: EBS_1359
NIS: NIS_0190
RHL: LPU83_pLPU83d0170(alg8-1) LPU83_pLPU83d1237(alg8-2)
MMED: Mame_02305
DSH: Dshi_1898
MALG: MALG_02777
SECH: B18_00670
NSH: GXM_00033
AAE: aq_1684(alg)
 » show all
Reference
PMID:8294014
  Authors
Maharaj R, May TB, Wang SK, Chakrabarty AM
  Title
Sequence of the alg8 and alg44 genes involved in the synthesis of alginate by Pseudomonas aeruginosa.
  Journal
Gene 136:267-9 (1993)
DOI:10.1016/0378-1119(93)90477-K
  Sequence
[pae:PA3541]
Reference
  Authors
Remminghorst U, Rehm BH
  Title
In vitro alginate polymerization and the functional role of Alg8 in alginate production by Pseudomonas aeruginosa.
  Journal
Appl Environ Microbiol 72:298-305 (2006)
DOI:10.1128/AEM.72.1.298-305.2006
LinkDB

KEGG   ORTHOLOGY: K19291
Entry
K19291                      KO                                     
Symbol
alg44
Name
mannuronan synthase [EC:2.4.1.33]
Pathway
map00051  Fructose and mannose metabolism
map01100  Metabolic pathways
map02025  Biofilm formation - Pseudomonas aeruginosa
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00051 Fructose and mannose metabolism
    K19291  alg44; mannuronan synthase
 09140 Cellular Processes
  09145 Cellular community - prokaryotes
   02025 Biofilm formation - Pseudomonas aeruginosa
    K19291  alg44; mannuronan synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.33  mannuronan synthase
     K19291  alg44; mannuronan synthase
Other DBs
RN: R08692
COG: COG5581
Genes
PAE: PA3542(alg44)
PAEV: N297_3665(alg44)
PAEI: N296_3665(alg44)
PAU: PA14_18550(alg44)
PAP: PSPA7_1603
PAG: PLES_14911(alg44)
PAF: PAM18_1444(alg44)
PNC: NCGM2_4667(alg44)
PAEB: NCGM1900_2742(alg44)
PDK: PADK2_06655
PAEP: PA1S_07710
PAEM: U769_07185
PAEL: T223_07425
PAEU: BN889_03912(alg44_1)
PAEG: AI22_26195
PAEC: M802_3662(alg44)
PAEO: M801_3530(alg44)
PMK: MDS_1080
PRE: PCA10_11860(alg44) PCA10_38210(alg44)
PPU: PP_1286(alg44)
PPF: Pput_4439
PPT: PPS_4405
PPI: YSA_03096
PPX: T1E_3166
PPUH: B479_22160
PPUT: L483_27245
PPUN: PP4_07980(alg44)
PPUD: DW66_4796
PMON: X969_21695
PMOT: X970_21330
PSB: Psyr_1061
PSYR: N018_20365
PVD: CFBP1590__4279(alg44)
PFL: PFL_1022(alg44)
PPRC: PFLCHA0_c10420(alg1)
PPRO: PPC_1060
PFE: PSF113_4754(alg44)
PFS: PFLU_0988
PFC: PflA506_0968(alg44)
PFB: VO64_4069
PMAN: OU5_2449
PFW: PF1751_v1c09460(alg44)
PEN: PSEEN3455 PSEEN4537(alg44)
PPUU: PputUW4_04473(alg44)
PKC: PKB_0966(alg44)
PSES: PSCI_0723
PSEM: TO66_05200
PSOS: POS17_1028
PANR: A7J50_0990
PSIL: PMA3_24595
PSEP: C4K39_1039
AVN: Avin_10950(alg44)
AVL: AvCA_10950(alg44)
AVD: AvCA6_10950(alg44)
ACX: Achr_31200(alg44)
ABO: ABO_0386(alg44)
TAU: Tola_1409
NIS: NIS_0189
SECH: B18_00675
BBA: Bd2717
BBAT: Bdt_2636
BBAC: EP01_09740
 » show all
Reference
PMID:8294014
  Authors
Maharaj R, May TB, Wang SK, Chakrabarty AM
  Title
Sequence of the alg8 and alg44 genes involved in the synthesis of alginate by Pseudomonas aeruginosa.
  Journal
Gene 136:267-9 (1993)
DOI:10.1016/0378-1119(93)90477-K
  Sequence
[pae:PA3542]
Reference
  Authors
Remminghorst U, Rehm BH
  Title
Alg44, a unique protein required for alginate biosynthesis in Pseudomonas aeruginosa.
  Journal
FEBS Lett 580:3883-8 (2006)
DOI:10.1016/j.febslet.2006.05.077
LinkDB

KEGG   ENZYME: 2.4.1.33
Entry
EC 2.4.1.33                 Enzyme                                 
Name
mannuronan synthase;
mannuronosyl transferase;
alginate synthase (incorrect);
alg8 (gene name);
alg44 (gene name);
GDP-D-mannuronate:alginate D-mannuronyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
GDP-alpha-D-mannuronate:mannuronan D-mannuronatetransferase
Reaction(IUBMB)
GDP-alpha-D-mannuronate + [(1->4)-beta-D-mannuronosyl]n = GDP + [(1->4)-beta-D-mannuronosyl]n+1 [RN:R08692]
Reaction(KEGG)
R08692;
(other) R03129
Substrate
GDP-alpha-D-mannuronate;
[(1->4)-beta-D-mannuronosyl]n
Product
GDP [CPD:C00035];
[(1->4)-beta-D-mannuronosyl]n+1
Comment
The enzyme catalyses the polymerization of beta-D-mannuronate residues into a mannuronan polymer, an intermediate in the biosynthesis of alginate. It is found in brown algae and in alginate-producing bacterial species from the Pseudomonas and Azotobacter genera.
History
EC 2.4.1.33 created 1972, modified 2015
Pathway
ec00051  Fructose and mannose metabolism
ec01100  Metabolic pathways
Orthology
K19290  mannuronan synthase
K19291  mannuronan synthase
Genes
PAEPA3541(alg8) PA3542(alg44)
PAEVN297_3664 N297_3665(alg44)
PAEIN296_3664 N296_3665(alg44)
PAUPA14_18550(alg44) PA14_18565(alg8)
PAPPSPA7_1603 PSPA7_1604
PAGPLES_14911(alg44) PLES_14921(alg8)
PAFPAM18_1444(alg44) PAM18_1445(alg8)
PNCNCGM2_4666(alg8) NCGM2_4667(alg44)
PAEBNCGM1900_2742(alg44) NCGM1900_2743(alg8)
PDKPADK2_06655 PADK2_06660
PSGG655_07090 G655_07095
PRPM062_18760 M062_18765
PAEPPA1S_07710 PA1S_07715
PAERPA1R_gp1359 PA1R_gp1360
PAEMU769_07185 U769_07190
PAELT223_07425 T223_07430
PAESSCV20265_1521 SCV20265_1522
PAEUBN889_03911 BN889_03912(alg44_1)
PAEGAI22_26190 AI22_26195
PAECM802_3661 M802_3662(alg44)
PAEOM801_3529 M801_3530(alg44)
PMYPmen_1029 Pmen_1030 Pmen_1675 Pmen_1676
PMKMDS_1080 MDS_1081
PREPCA10_11860(alg44) PCA10_11870(alg8) PCA10_38210(alg44) PCA10_38220(alg8)
PFUWKF707C_11150 KF707C_11160
PALCA0T30_03900 A0T30_03905 A0T30_11810 A0T30_11815
PCQPcP3B5_50690 PcP3B5_50700
PMUIG4G71_08965 G4G71_08970
PPUPP_1286(alg44) PP_1287(alg8)
PPFPput_4438 Pput_4439
PPGPputGB1_4562 PputGB1_4563
PPWPputW619_0887 PputW619_0888
PPTPPS_4404 PPS_4405
PPBPPUBIRD1_4274 PPUBIRD1_4275
PPIYSA_03094 YSA_03096
PPXT1E_3166 T1E_3167
PPUHB479_22155 B479_22160
PPUTL483_27240 L483_27245
PPUNPP4_07980(alg44) PP4_07990(alg8)
PPUDDW66_4795 DW66_4796
PFVPsefu_3882 Psefu_3883
PMONX969_21690 X969_21695
PMOTX970_21325 X970_21330
PMOSO165_013050 O165_013055 O165_018565 O165_018570
PPJRK21_00117 RK21_00118
PMOLCLJ08_08385 CLJ08_08390 CLJ08_22915 CLJ08_22920
PSTPSPTO_1241 PSPTO_1242
PSBPsyr_1061 Psyr_1062
PSYRN018_20360 N018_20365
PSPPSPPH_1116 PSPPH_1117
PSAVPSA3335_09085 PSA3335_09090
PAMGBKM19_008075 BKM19_008080
PCIPCH70_10110 PCH70_10120
PAVLBKM03_07010 BKM03_07015
PVDCFBP1590__4278(alg8) CFBP1590__4279(alg44)
PCABJGS08_19530(alg8) JGS08_19535
PFLPFL_1022(alg44) PFL_1023
PPRCPFLCHA0_c10420(alg1) PFLCHA0_c10430(alg2)
PPROPPC_1060 PPC_1061
PFEPSF113_4753(alg8) PSF113_4754(alg44)
PFNHZ99_11920 HZ99_11925
PFOPfl01_0958 Pfl01_0959
PFSPFLU_0988 PFLU_0989
PFCPflA506_0968(alg44) PflA506_0969
PPZH045_01680 H045_01685
PFBVO64_4069 VO64_4070
PMANOU5_2448 OU5_2449
PTVAA957_09050 AA957_09055
PCGAXG94_09980 AXG94_09985
PVRPverR02_05215 PverR02_05225
PAZOAYR47_12220 AYR47_12225
POIBOP93_04725 BOP93_04730
PRXHRH33_05795 HRH33_05800
PFWPF1751_v1c09460(alg44) PF1751_v1c09470(alg8)
PFFPFLUOLIPICF703395 PFLUOLIPICF703400
PFXA7318_04280 A7318_04285
PENPSEEN3455 PSEEN4536(alg8) PSEEN4537(alg44)
PLULFOB45_10135
PBAPSEBR_a4591 PSEBR_a4592
PBCCD58_24310 CD58_24315
PPUUPputUW4_04472(alg8) PputUW4_04473(alg44)
PDRH681_19430 H681_19435
PSVPVLB_04225 PVLB_04230 PVLB_15475 PVLB_15480
PSKU771_06115 U771_06120
PKCPKB_0966(alg44) PKB_0967(alg8)
PCHEY04_04510 EY04_04515
PCZPCL1606_50720 PCL1606_50730
PCPJM49_25130 JM49_25135
PFZAV641_18515 AV641_18520
PLQAA042_15140 AA042_15145
PALKPSAKL28_08720 PSAKL28_08730 PSAKL28_31820 PSAKL28_31830
PPVNJ69_13835 NJ69_13840
PSESPSCI_0722 PSCI_0723
PSEMTO66_05200 TO66_05205
PSECCCOS191_0910(alg44) CCOS191_0911(alg8a) CCOS191_2027 CCOS191_2028(alg8b)
PPSYAOC04_15735 AOC04_15740
PSOSPOS17_1028 POS17_1029
PKRAYO71_07530 AYO71_07535
PFKPFAS1_06335 PFAS1_06340
PANRA7J50_0990 A7J50_0991
PSETTHL1_1266 THL1_1267 THL1_3802 THL1_3803
PSILPMA3_24590 PMA3_24595
PYMAK972_0836 AK972_0837
PKEDLD99_05150 DLD99_05155
PALLUYA_04875 UYA_04880 UYA_15330 UYA_15335
PUMHGP31_04965 HGP31_04970
POJPtoMrB4_25970 PtoMrB4_25980(alg8_1) PtoMrB4_46200(alg8_2) PtoMrB4_46210
PGGFX982_00688 FX982_00689
PGYAWU82_19085 AWU82_19090 AWU82_28995 AWU82_29000
PLALFXN65_05545 FXN65_05550
PSEJHNQ25_10155 HNQ25_10160 HNQ25_19285 HNQ25_19290
PVKEPZ47_24335 EPZ47_24340
PEZHWQ56_05040 HWQ56_05045
PBZGN234_24005 GN234_24010
PKHJLK41_05210 JLK41_05215(alg8)
PTYJWV26_01380 JWV26_01385 JWV26_15955 JWV26_15960
PATAJWU58_22755(alg8) JWU58_22760
PDWBV82_3322 BV82_3323 BV82_4691(alg8) BV82_4692
PZEHU754_006260 HU754_006265(alg8)
PMOEHV782_005840 HV782_005845(alg8)
PCAMHNE05_19960 HNE05_19965
PSEPC4K39_1039 C4K39_1040
PVWHU752_027435(alg8) HU752_027440
PTRTHU722_0006155 HU722_0006160(alg8)
PXAKSS93_07780 KSS93_07785
PSAMHU731_020995 HU731_021000(alg8)
PTZHU718_005900 HU718_005905(alg8)
PQIKH389_10470 KH389_10475 KH389_22250(alg8) KH389_22255
PSHHHU773_022840(alg8) HU773_022845
PXNHU772_004410 HU772_004415 HU772_009420 HU772_009425
PHVHU739_012715(alg8) HU739_012720
PPRGHU725_004075 HU725_004080 HU725_014405 HU725_014410
PANHHU763_020335 HU763_020340
PASGKSS96_05685 KSS96_05690(alg8)
PFAKKSS94_04605 KSS94_04610
PMUYKSS95_07465 KSS95_07470(alg8) KSS95_11750 KSS95_11755
PMAMKSS90_04825 KSS90_04830 KSS90_09895 KSS90_09900
PAZEKSS91_23850(alg8) KSS91_23855
PALVKSS97_06285 KSS97_06290(alg8)
PPHABVH74_05220 BVH74_05225
PORYEJA05_04790 EJA05_04795
PWZJ7655_04680 J7655_04685(alg8)
PTKEXN22_12370 EXN22_12375 EXN22_22960 EXN22_22965
PTWTUM18999_50130(alg8) TUM18999_50140
PZAHU749_024145(alg8) HU749_024150
PIEHU724_005660 HU724_005665(alg8)
PWYHU734_005470 HU734_005475
AVNAvin_10950(alg44) Avin_10960(alg8)
AVLAvCA_10950(alg44) AvCA_10960(alg8)
AVDAvCA6_10950(alg44) AvCA6_10960(alg8)
ACXAchr_31190(alg8) Achr_31200(alg44)
MSRAU15_13670 AU15_13675
PSPOPSPO_a3133
HMIsoil367_08795 soil367_08800
CEGD0C16_10995 D0C16_11000
HHUAR456_14305
HSIBOX17_03120 BOX17_03125
HALOBWR19_06110
HCAMI4484_14790
HSXHNO51_06545 HNO51_14515 HNO51_14520
HSVHNO53_07210 HNO53_15195 HNO53_15200
HTXEKK97_07660 EKK97_16400 EKK97_16405
PAURFGL86_16320 FGL86_16325
ABOABO_0385(alg8) ABO_0386(alg44)
ALNAS19_03980 AS19_03990
GSNYC6258_00964 YC6258_00965
OMEOLMES_4478 OLMES_4479
TAUTola_1408 Tola_1409
REVHUE57_12995 HUE57_13030
ENMEBS_1359
PLGNCTC10937_04284 NCTC10937_04285
RTEAHK414_18200
NISNIS_0189 NIS_0190
CMEDFE773_08545 FE773_08550
CPAFC6V80_01895 C6V80_01900
ARUIG6M88_17200 G6M88_17205
RHLLPU83_pLPU83d0169 LPU83_pLPU83d0170(alg8-1) LPU83_pLPU83d1237(alg8-2)
RGRFZ934_26055 FZ934_26060
MAADAZF01_02680
MMEDMame_02305
MLUTJET14_03730 JET14_03735
LABRCHH27_23025
LABPFJ695_16710 FJ695_16715
DSHDshi_1898
REDroselon_01183
MALGMALG_02777
YPACCEW88_18630 CEW88_18635
YANAYJ57_24985 AYJ57_24990
SULZC1J03_23170
TOMBWR18_20770
THAACFI11_06315 CFI11_06495
THWBMG03_12920
GEHHYN69_02905
POZI0K15_05940
GCEKYE46_09225
SECHB18_00670 B18_00675
SROEJL101_006580
BBABd2717
BBATBdt_2636
BBACEP01_09740
PARHI5S86_05470 I5S86_05475
HAOPCC7418_0426
NSHGXM_00033
MFFMFFC18_28840
AAEaq_1684(alg)
TAMTheam_0794 Theam_0795
 » show all
Reference
1  [PMID:5954796]
  Authors
Lin TY, Hassid WZ.
  Title
Pathway of algnic acid synthesis in the marine brown alga, Fucus gardneri Silva.
  Journal
J Biol Chem 241:5284-97 (1966)
Reference
2  [PMID:16391057]
  Authors
Remminghorst U, Rehm BH
  Title
In vitro alginate polymerization and the functional role of Alg8 in alginate production by Pseudomonas aeruginosa.
  Journal
Appl Environ Microbiol 72:298-305 (2006)
DOI:10.1128/AEM.72.1.298-305.2006
  Sequence
[pae:PA3541]
Reference
3  [PMID:18524915]
  Authors
Oglesby LL, Jain S, Ohman DE
  Title
Membrane topology and roles of Pseudomonas aeruginosa Alg8 and Alg44 in alginate  polymerization.
  Journal
Microbiology 154:1605-15 (2008)
DOI:10.1099/mic.0.2007/015305-0
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.33
IUBMB Enzyme Nomenclature: 2.4.1.33
ExPASy - ENZYME nomenclature database: 2.4.1.33
BRENDA, the Enzyme Database: 2.4.1.33
CAS: 37257-31-1
LinkDB

KEGG   REACTION: R08692
Entry
R08692                      Reaction                               
Name
GDP-D-mannuronate:Poly(beta-D-mannuronate) beta-D-mannuronyltransferase
Definition
GDP-D-mannuronate + (beta-D-Mannuronate)n <=> (beta-D-Mannuronate)n + GDP
Equation
Reaction class
RC00005  C00035_C00976
Enzyme
Pathway
rn00051  Fructose and mannose metabolism
rn01100  Metabolic pathways
Orthology
K19290  mannuronan synthase [EC:2.4.1.33]
K19291  mannuronan synthase [EC:2.4.1.33]
Other DBs
RHEA: 46879
LinkDB

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