KEGG   ORTHOLOGY: K19412
Entry
K19412                      KO                                     
Symbol
argX
Name
glutamate---[amino group carrier protein] ligase [EC:6.3.2.60]
Pathway
map00220  Arginine biosynthesis
map01100  Metabolic pathways
map01210  2-Oxocarboxylic acid metabolism
map01230  Biosynthesis of amino acids
Module
M00763  Ornithine biosynthesis, mediated by LysW, glutamate => ornithine
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00220 Arginine biosynthesis
    K19412  argX; glutamate---[amino group carrier protein] ligase
Enzymes [BR:ko01000]
 6. Ligases
  6.3  Forming carbon-nitrogen bonds
   6.3.2  Acid-D-amino-acid ligases (peptide synthases)
    6.3.2.60  glutamate---[amino group carrier protein] ligase
     K19412  argX; glutamate---[amino group carrier protein] ligase
Other DBs
RN: R10929
COG: COG0189
Genes
MIG: Metig_0633
MVN: Mevan_0912
MOK: Metok_1139
METF: CFE53_00515
FAI: FAD_1383
IHO: Igni_1403
IIS: EYM_00645
PFM: Pyrfu_0756
PDL: Pyrde_0370
STO: STK_15050(argE)
SOH: D1869_08455(lysX)
SSO: SSO0645(rimK-2)
SOL: Ssol_1704
SSOA: SULA_1732(lysX)
SSOL: SULB_1733(lysX)
SSOF: SULC_1731(lysX)
SCAS: SACC_03370
SAI: Saci_1621
SID: M164_1489
SII: LD85_1699
SIH: SiH_1458
SIR: SiRe_1368
SIC: SiL_1371
SULE: GFS03_08525(lysX)
SULO: GFS33_00300(lysX)
SULL: EWF20_09625(lysX)
MSE: Msed_1991
MCN: Mcup_0295
MHK: DFR87_08550(lysX)
MPRU: DFR88_08220(lysX)
MTEN: GWK48_03775(lysX)
AHO: Ahos_1105
ABRI: DFR85_09220(lysX)
ASUL: DFR86_08280(lysX)
AAMB: D1866_00395(lysX)
ACIH: HS5_25000
SAZO: D1868_05495(lysX)
CSTY: KN1_27050
STEP: IC006_1362
PYR: P186_2397
TNE: Tneu_0136
TUZ: TUZN_1890
VDI: Vdis_0196
VMO: VMUT_1535
NAA: Nps_00535
 » show all
Reference
  Authors
Ouchi T, Tomita T, Horie A, Yoshida A, Takahashi K, Nishida H, Lassak K, Taka H, Mineki R, Fujimura T, Kosono S, Nishiyama C, Masui R, Kuramitsu S, Albers SV, Kuzuyama T, Nishiyama M
  Title
Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus.
  Journal
Nat Chem Biol 9:277-83 (2013)
DOI:10.1038/nchembio.1200
  Sequence
[sai:Saci_1621]
LinkDB

KEGG   ORTHOLOGY: K25032
Entry
K25032                      KO                                     
Symbol
K25032
Name
alpha-aminoadipate-LysW ligase/glutamate-LysW ligase [EC:6.3.2.43 6.3.2.60]
Pathway
map00220  Arginine biosynthesis
map00300  Lysine biosynthesis
map01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00300 Lysine biosynthesis
    K25032  K25032; alpha-aminoadipate-LysW ligase/glutamate-LysW ligase
   00220 Arginine biosynthesis
    K25032  K25032; alpha-aminoadipate-LysW ligase/glutamate-LysW ligase
Enzymes [BR:ko01000]
 6. Ligases
  6.3  Forming carbon-nitrogen bonds
   6.3.2  Acid-D-amino-acid ligases (peptide synthases)
    6.3.2.43  [amino-group carrier protein]---L-2-aminoadipate ligase
     K25032  K25032; alpha-aminoadipate-LysW ligase/glutamate-LysW ligase
    6.3.2.60  glutamate---[amino group carrier protein] ligase
     K25032  K25032; alpha-aminoadipate-LysW ligase/glutamate-LysW ligase
Other DBs
RN: R09775 R10929
COG: COG0189
Genes
PFU: PF1682
PFI: PFC_10195
PHO: PH1721(PH1721)
PAB: PAB0290(rimK)
PYN: PNA2_0329 PNA2_0499
PYS: Py04_1636
TKO: TK0278
THS: TES1_0507
THV: ADU37_CDS10050
TCH: CHITON_1979
Reference
  Authors
Yoshida A, Tomita T, Atomi H, Kuzuyama T, Nishiyama M
  Title
Lysine Biosynthesis of Thermococcus kodakarensis with the Capacity to Function as an Ornithine Biosynthetic System.
  Journal
J Biol Chem 291:21630-21643 (2016)
DOI:10.1074/jbc.M116.743021
  Sequence
[tko:TK0278]
LinkDB

KEGG   ENZYME: 6.3.2.60
Entry
EC 6.3.2.60                 Enzyme                                 
Name
glutamate---[amino group carrier protein] ligase;
argX (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
Sysname
L-glutamate:an [amino-group carrier protein]-C-terminal-L-glutamate ligase (ADP-forming)
Reaction(IUBMB)
ATP + L-glutamate + an [amino-group carrier protein]-C-terminal-L-glutamate = ADP + phosphate + an [amino-group carrier protein]-C-terminal-gamma-(L-glutamyl)-L-glutamate [RN:R10929]
Reaction(KEGG)
R10929
Substrate
ATP [CPD:C00002];
L-glutamate [CPD:C00025];
[amino-group carrier protein]-C-terminal-L-glutamate
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
[amino-group carrier protein]-C-terminal-gamma-(L-glutamyl)-L-glutamate
Comment
The enzyme, originally characterized from the archaeon Sulfolobus acidocaldarius, is involved in L-arginine biosynthesis. The enzyme from the archaeon Thermococcus kodakarensis is bifunctional and also catalyses the activity of EC 6.3.2.43, [amino-group carrier protein]---L-2-aminoadipate ligase.
History
EC 6.3.2.60 created 2021
Pathway
ec00220  Arginine biosynthesis
ec01100  Metabolic pathways
Orthology
K19412  glutamate---[amino group carrier protein] ligase
K25032  alpha-aminoadipate-LysW ligase/glutamate-LysW ligase
Genes
MIGMetig_0633
MVNMevan_0912
MOKMetok_1139
METFCFE53_00515
PFUPF1682
PFIPFC_10195
PHOPH1721(PH1721)
PABPAB0290(rimK)
PYNPNA2_0329 PNA2_0499
PYSPy04_1636
TKOTK0278
THSTES1_0507
THVADU37_CDS10050
TCHCHITON_1979
FAIFAD_1383
IHOIgni_1403
IISEYM_00645
PFMPyrfu_0756
PDLPyrde_0370
STOSTK_15050(argE)
SOHD1869_08455(lysX)
SSOSSO0645(rimK-2)
SOLSsol_1704
SSOASULA_1732(lysX)
SSOLSULB_1733(lysX)
SSOFSULC_1731(lysX)
SSHIJ5U23_02762
SCASSACC_03370
SAISaci_1621
SACNSacN8_07870
SACRSacRon12I_07880
SACSSUSAZ_07680
SISLS215_1600
SIAM1425_1492
SIMM1627_1607
SIDM164_1489
SIYYG5714_1496
SINYN1551_1340
SIILD85_1699
SIHSiH_1458
SIRSiRe_1368
SICSiL_1371
SULABFU36_02820
SULEGFS03_08525(lysX)
SULOGFS33_00300(lysX)
SULLEWF20_09625(lysX)
MSEMsed_1991
MCNMcup_0295
MHKDFR87_08550(lysX)
MPRUDFR88_08220(lysX)
MTENGWK48_03775(lysX)
MJNMjAS7_0291
AHOAhos_1105
AMANB6F84_09595
ABRIDFR85_09220(lysX)
ASULDFR86_08280(lysX)
AAMBD1866_00395(lysX)
ACIHHS5_25000
SACDHS1genome_0140
SAZOD1868_05495(lysX)
CSTYKN1_27050
STEPIC006_1362
PYRP186_2397
TNETneu_0136
PYWPYWP30_00148
TUZTUZN_1890
VDIVdis_0196
VMOVMUT_1535
CSUCSUB_C0076
NAANps_00535
 » show all
Reference
1  [PMID:23434852]
  Authors
Ouchi T, Tomita T, Horie A, Yoshida A, Takahashi K, Nishida H, Lassak K, Taka H, Mineki R, Fujimura T, Kosono S, Nishiyama C, Masui R, Kuramitsu S, Albers SV, Kuzuyama T, Nishiyama M
  Title
Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus.
  Journal
Nat Chem Biol 9:277-83 (2013)
DOI:10.1038/nchembio.1200
  Sequence
[sai:Saci_1621]
Reference
2  [PMID:27566549]
  Authors
Yoshida A, Tomita T, Atomi H, Kuzuyama T, Nishiyama M
  Title
Lysine Biosynthesis of Thermococcus kodakarensis with the Capacity to Function as an Ornithine Biosynthetic System.
  Journal
J Biol Chem 291:21630-21643 (2016)
DOI:10.1074/jbc.M116.743021
  Sequence
[tko:TK0278]
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.60
IUBMB Enzyme Nomenclature: 6.3.2.60
ExPASy - ENZYME nomenclature database: 6.3.2.60
BRENDA, the Enzyme Database: 6.3.2.60
LinkDB

KEGG   REACTION: R10929
Entry
R10929                      Reaction                               
Name
LysW:L-glutamate ligase (ADP-forming)
Definition
L-Glutamate + alpha-Aminoadipate carrier protein LysW + ATP <=> LysW-L-glutamate + ADP + Orthophosphate
Equation
Reaction class
RC00002  C00002_C00008
RC00064  C00025_C20948
RC00090  C19885_C20948
Enzyme
Pathway
rn00220  Arginine biosynthesis
rn01100  Metabolic pathways
rn01210  2-Oxocarboxylic acid metabolism
rn01230  Biosynthesis of amino acids
Module
M00763  Ornithine biosynthesis, mediated by LysW, glutamate => ornithine
Orthology
K19412  glutamate---[amino group carrier protein] ligase [EC:6.3.2.60]
K25032  alpha-aminoadipate-LysW ligase/glutamate-LysW ligase [EC:6.3.2.43 6.3.2.60]
Other DBs
RHEA: 52627
LinkDB

DBGET integrated database retrieval system