KEGG ORTHOLOGY: K19562

ORTHOLOGY: K19562

Entry

K19562                      KO

Symbol

BIO3-BIO1

Name

bifunctional dethiobiotin synthetase / spermidine---8-amino-7-oxononanoate transaminase [EC:6.3.3.3 2.6.1.127]

Pathway

map00780

Biotin metabolism

map01100

Metabolic pathways

map01240

Biosynthesis of cofactors

Module

M00123

Biotin biosynthesis, pimeloyl-ACP/CoA => biotin

Reaction

R03182

7,8-diaminononanoate:carbon-dioxide cyclo-ligase

R03231

S-adenosyl-L-methionine:8-amino-7-oxononanoate aminotransferase

R12933

R12934

R13517

spermidine:8-amino-7-oxononanoate aminotransferase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09108 Metabolism of cofactors and vitamins
   00780 Biotin metabolism
    K19562  BIO3-BIO1; bifunctional dethiobiotin synthetase / spermidine---8-amino-7-oxononanoate transaminase
Enzymes [BR:ko01000]
2. Transferases
  2.6  Transferring nitrogenous groups
   2.6.1  Transaminases
    2.6.1.127  spermidine---8-amino-7-oxononanoate transaminase
     K19562  BIO3-BIO1; bifunctional dethiobiotin synthetase / spermidine---8-amino-7-oxononanoate transaminase
6. Ligases
  6.3  Forming carbon-nitrogen bonds
   6.3.3  Cyclo-ligases
    6.3.3.3  dethiobiotin synthase
     K19562  BIO3-BIO1; bifunctional dethiobiotin synthetase / spermidine---8-amino-7-oxononanoate transaminase

Other DBs

GO:

0004141

Genes

ATH:	AT5G57590(BIO1)

ALY:

9302294

CRB:

17875755

CSAT:

104726316 104734606 104761844

EUS:	EUTSA_v10012677mg

BRP:	103845194 103856746 103871196

BNA:	106374525 106387199 106402127 106404343 106422454 106436665 125593003

BOE:	106319268 106336470 106344307

RSZ:

THJ:

CPAP:

CIT:

CIC:	CICLE_v10007423mg

PVY:

MINC:

TCC:

GRA:

GHI:	107944094 121212636

GAB:

HSYR:

DZI:

EGR:

RARG:

GMX:

GSJ:

PVU:

VRA:

VAR:

VUN:

VUM:

CCAJ:

APRC:

MTR:

TPRA:

CAM:

PSAT:

VVO:

LJA:

ADU:

AIP:

AHF:	112698453 112723529

LANG:

109362250

PCIN:

129319698

QSA:	O6P43_002883

FVE:

RCN:

PPER:

PMUM:

PAVI:

PDUL:

MDM:

MSYL:

PXB:

PCOX:

ZJU:

MNT:

CSAV:

HLP:

CSV:

CMO:

BHJ:

MCHA:

CMAX:

CMOS:

CPEP:

RCU:

MEAN:

JCU:

HBR:

MESC:

POP:

PEU:

PALZ:

PNZ:

JRE:

CILL:

CAVE:

AGLU:

QSU:

QLO:

CSAA:

TWL:

VVI:

VRI:

SLY:

SPEN:

SOT:

SSTN:

SDUL:

CANN:

LBB:

NTA:	107768222 107788123

NSY:

NTO:

NAU:

INI:

ITR:

SIND:

EGT:

SSPL:

SMIL:

SHIS:

APAN:

PEBU:

PTAB:

CARB:

HAN:

BHW:	143567198 143579959

ECAD:

LSV:

CCAV:

DCR:

AGRV:

CSIN:

RVL:

AEW:

DLT:

BVG:

SOE:

CQI:	110720644 110721095

ATRI:

130814688

SLAF:

141597216

MOF:	131147167 131156979

NNU:

MING:

TSS:

PSOM:

OSA:	4345056(BIO1)

DOSA:

OBR:

OGL:

BDI:

ATS:

TDC:	119316400 119323887

TAES:

123132596 123136715 123144196

TUA:

HVG:

LPER:

LRD:

SBI:

ZMA:

MFLO:

SITA:

SVS:

PVIR:

PHAI:

PAUA:

TANG:

PDA:

EGU:

MUS:

ZOF:	121967306 122025747 122025755 122038531

DCT:

PEQ:

AOF:

MSIN:

ACAK:

NCOL:

ATR:

CJF:

SMO:	SELMODRAFT_413615 SELMODRAFT_416628

PPP:

112294585

CRE:	CHLRE_06g277200v5

VCN:	VOLCADRAFT_40597

CSL:	COCSUDRAFT_810

CVR:	CHLNCDRAFT_145418

APRO:

F751_0478

OLU:	OSTLU_591 OSTLU_597

OTA:	OT_ostta13g01490

BPG:	Bathy17g01920(DTB)

MIS:	MICPUN_59263

MPP:	MICPUCDRAFT_34980

CME:	CYME_CMG023C

GSL:	Gasu_42400

CCP:	CHC_T00002287001

YLI:	2911900(YALI2_E01336g)

PAN:	PODANSg531

PBEL:

PPSD:

PPSP:

PPSA:

TTT:	THITE_2058801

MTM:	MYCTH_2299183

SSCK:

SPSK_03105

FGR:	FGSG_09048

FPU:	FPSE_07330

FPOA:

FPOAC1_012695

FVN:	FVRRES_13921

FVR:	FVEG_15894

FOX:	FOXG_19952

NHE:	NECHADRAFT_46109

FFC:	NCS54_00993900

FKR:	NCS57_00988200

FMU:	J7337_009228

TRE:	TRIREDRAFT_65198

TRR:	M419DRAFT_85598

TATV:

25775682(TrAtP1_010389)

MAJ:

MAA_05458

MBRN:

26238989(BIO3-BIO1)

PCHM:

VFPPC_03318

UVI:	66061315(UV8b_00537)

CMT:

CCM_08605

AMUS:

LMH87_000367

PLJ:	28886042(PLICBS_007553)

PTKZ:

JDV02_002469

DCON:

63718180(DCS_05537)

VAL:	VDBG_07632

VDA:	VDAG_09354

CFJ:	CFIO01_05094

CLUP:

CLUP02_08485

CHIG:

CH63R_07606

CDET:

87944812(CDEST_08309)

SAPO:

SAPIO_CDS9256

ELA:	UCREL1_348

PFY:	PFICI_05463

SSL:	SS1G_08439

BFU:	BCIN_12g02430

MBE:

MBM_09317

PSCO:

LY89DRAFT_683081

ANI:	ANIA_06644(biA)

AFM:	AFUA_6G03660

ACT:	ACLA_096920

NFI:	NFIA_050160

AOR:	AO090009000590

ANG:	An15g01990

AFV:	AFLA_010783

ALUC:

AKAW2_60496A

ACHE:

ACHE_50095S

APUU:

APUU_10607S

PCS:	N7525_009721

PDP:	PDIP_16970

POU:	POX_f07633

PPSF:

300788837(PFLUO_LOCUS3145)

TMF:	EYB26_000016

TRG:	TRUGW13939_04272

CIM:	CIMG_01317

CPW:	9691339(D8B26_001861)

URE:	UREG_01244

ABE:

ARB_01948

TVE:

TRV_04743

PBL:	PAAG_08156

PBN:	PADG_08456

AJE:	HCAG_05726

BGH:	BDBG_02033

PNO:	SNOG_15125

PTE:

PTT_18503

PTRR:

6340114(PtrM4_004240)

BZE:	COCCADRAFT_97800

BSC:	COCSADRAFT_40231

BOR:	COCMIDRAFT_95822

AALT:

CC77DRAFT_983495

ADAC:

96084455(ACET3X_004133)

ARAB:

EKO05_0009218

ZTR:	MYCGRDRAFT_111308

PFJ:	MYCFIDRAFT_44846

FFU:	CLAFUR5_12855

CBET:

CB0940_05363

BCOM:

BAUCODRAFT_343702

NPA:	UCRNP2_4321

CNE:

CNB00630

CNB:

CNBB5070

CNG:	CNAG_06739

CGI:	CGB_B9110W

CDEU:

CNBG_6120

CDEC:

89989885(IAS62_003112)

CTEG:

91993633(I308_106780)

CBAS:

91983049(I312_103003)

CDEP:

91088438(L203_104228)

KMG:	30164642(I203_107936)

KNE:	92177833(IAR55_000573)

KDJ:	28967681(I303_103964)

KBI:	30213106(I302_105426)

KPIN:

30169732(I206_103635)

KSH:	87955994(IL334_003863)

KER:	91107347(V865_008546)

KSN:	43592123(CI109_100140)

KEP:	91090782(L201_000110)

TMS:	TREMEDRAFT_30257

CCAC:

CcaHIS019_0508780(bioDA)

TVS:	TRAVEDRAFT_125534

DSQ:	DICSQDRAFT_133242

PCO:	PHACADRAFT_171054

SHS:	STEHIDRAFT_128037

HIR:	HETIRDRAFT_316475

PSQ:	PUNSTDRAFT_92463

ADL:	AURDEDRAFT_100686

FME:	FOMMEDRAFT_102518

GTR:	GLOTRDRAFT_64423

RSX:	RhiXN_02950

LBC:	LACBIDRAFT_190389

CCI:	CC1G_06303

ABP:	AGABI1DRAFT75033(AGABI1DRAFT_75033)

ABV:	AGABI2DRAFT201364(AGABI2DRAFT_201364)

PCUB:

JR316_0006218

MPR:	MPER_02846

MRR:	Moror_4589

SCM:	SCHCO_02625758(SCHCODRAFT_02625758)

CPUT:

CONPUDRAFT_100025

SLA:	SERLADRAFT_438251

WSE:	WALSEDRAFT_52570

WIC:	J056_000827

UMA:	UMAG_02915

PFP:	PFL1_01329

SGRA:

MGL:

MRT:

MSYM:

PGR:	PGTG_14186

PTRC:

PtA15_9A440

PSTR:

Pst134EA_021341

MLR:	MELLADRAFT_45909

RIE:	OCT59_029710

PTI:	PHATRDRAFT_19762

PIF:	PITG_06454

PSOJ:

PHYSODRAFT_258968

BLAC:

94344572(CCR75_000795)

SPAR:

SPRG_19814

GTT:	GUITHDRAFT_161318

SBF:	JCM31447_25940

» show all

Reference

PMID:22547782

Authors

Cobessi D, Dumas R, Pautre V, Meinguet C, Ferrer JL, Alban C

Title

Biochemical and structural characterization of the Arabidopsis bifunctional enzyme dethiobiotin synthetase-diaminopelargonic acid aminotransferase: evidence for substrate channeling in biotin synthesis.

Journal

Plant Cell 24:1608-25 (2012)
DOI:10.1105/tpc.112.097675

Sequence

[ath:AT5G57590]

Reference

PMID:40028677

Authors

Noble CG, Hollinshead T, Kende A, Langford MP, Lim PP, Linney E, Mattocks J, Swindale LY, Green K.

Title

The plant Diaminopelargonic acid aminotransferase uses spermidine as its amino donor.

Journal

Plant J 121:e70076 (2025)
DOI:10.1111/tpj.70076

Sequence

[ath:AT5G57590]

LinkDB

ENZYME: 2.6.1.127

Entry

EC 2.6.1.127                Enzyme

Name

spermidine---8-amino-7-oxononanoate transaminase;
BIO3-BIO1 (gene name)

Class

Transferases;
Transferring nitrogenous groups;
Transaminases

Sysname

spermidine:8-amino-7-oxononanoate aminotransferase

Reaction(IUBMB)

spermidine + 8-amino-7-oxononanoate = N-(3-aminopropyl)-4-aminobutanal + 7,8-diaminononanoate [RN:R13517]

Reaction(KEGG)

R13517

Substrate

spermidine [CPD:C00315];
8-amino-7-oxononanoate [CPD:C01092]

Product

N-(3-aminopropyl)-4-aminobutanal [CPD:C23055];
7,8-diaminononanoate [CPD:C01037]

Comment

A pyridoxal 5'-phosphate enzyme. The enzyme, characterized from the plant Arabidopsis thaliana, differs from enzymes that catalyse a similar reaction by using spermidine as the amino group donor. The enzyme from Arabidopsis thaliana is bifunctional and also catalyses EC 6.3.3.3, dethiobiotin synthase. cf. EC 2.6.1.62, adenosylmethionine---8-amino-7-oxononanoate transaminase, and EC 2.6.1.105, lysine---8-amino-7-oxononanoate transaminase.

History

EC 2.6.1.127 created 2025

Pathway

ec00780

Biotin metabolism

ec01100

Metabolic pathways

Orthology

K19562

bifunctional dethiobiotin synthetase / spermidine---8-amino-7-oxononanoate transaminase

Genes

ATH:	AT5G57590(BIO1)

ALY:

9302294

CRB:

17875755

CSAT:

104726316 104734606 104761844

EUS:	EUTSA_v10012677mg

BRP:	103845194 103856746 103871196

BNA:	106374525 106387199 106402127 106404343 106422454 106436665 125593003

BOE:	106319268 106336470 106344307

RSZ:

108828750

THJ:

104811804

CPAP:

110811081

CIT:

102624762

CIC:	CICLE_v10007423mg

PVY:

116111982

MINC:

123193828 123200037

TCC:

18589183

GRA:

105783477

GHI:	107944094 121212636

GAB:

108453715

HSYR:

120116531

DZI:

111303418

EGR:

104435978

RARG:

115744620

GMX:

100778549

GSJ:

114382800

PVU:

137807356

VRA:

106769165

VAR:

108322165

VUN:

114179514

VUM:

124835072

CCAJ:

109808728

APRC:

113847062

MTR:

11446047

TPRA:

123908573

CAM:

101488228

PSAT:

127138534

VVO:

131628200

LJA:

130710295

ADU:

107459919

AIP:

107642154

AHF:	112698453 112723529

LANG:

109362250

PCIN:

129319698

QSA:	O6P43_002883

FVE:

101311473

RCN:

112200682

PPER:

18789985

PMUM:

103323503

PAVI:

110752549

PDUL:

117616029 117630474

MDM:

103447394

MSYL:

126596178

PXB:

103962395

PCOX:

137737579

ZJU:

107414560

MNT:

21405690

CSAV:

115695820

HLP:

133783104

CSV:

101216016

CMO:

103494289

BHJ:

120070871

MCHA:

111007086

CMAX:

111495968

CMOS:

111435969

CPEP:

111792680

RCU:

8277910

MEAN:

126668024

JCU:

105648016

HBR:

110661201

MESC:

110617283

POP:

18100519

PEU:

105116015

PALZ:

118057946

PNZ:

133690290

JRE:

108998892

CILL:

122317059

CAVE:

132190968

AGLU:

133871908

QSU:

112013953

QLO:

115977399

CSAA:

142622584

TWL:

119996041

VVI:

100259784

VRI:

117925067

SLY:

101244782

SPEN:

107026492

SOT:

102579954

SSTN:

125876335

SDUL:

129891640

CANN:

107877502

LBB:

132602809

NTA:	107768222 107788123

NSY:

104234088

NTO:

104098085

NAU:

109234603

INI:

109160670

ITR:

115995629

SIND:

105155673

EGT:

105952309

SSPL:

121741893 121745617

SMIL:

131004901

SHIS:

125216411

APAN:

127244246

PEBU:

140818341

PTAB:

142505179

CARB:

113693770 113697022

HAN:

110929120

BHW:	143567198 143579959

ECAD:

122601947

LSV:

111879506

CCAV:

112500106

DCR:

108215280

AGRV:

141721168

CSIN:

114267757

RVL:

131333758

AEW:

130753208

DLT:

127806894

BVG:

104902421

SOE:

110805202

CQI:	110720644 110721095

ATRI:

130814688

SLAF:

141597216

MOF:	131147167 131156979

NNU:

104603186

MING:

122070994

TSS:

122658621

PSOM:

113297725 113301475

OSA:	4345056(BIO1)

DOSA:

Os08g0245400

OBR:

102716523

OGL:

127782758

BDI:

100827183

ATS:

109759698

TDC:	119316400 119323887

TAES:

123132596 123136715 123144196

TUA:

125517376

HVG:

123401312

LPER:

127301088

LRD:

124686122

SBI:

8081870

ZMA:

100382888

MFLO:

136540440

SITA:

101776659

SVS:

117859792

PVIR:

120678685 120711920

PHAI:

112897207

PAUA:

133899473

TANG:

140766706

PDA:

103714680

EGU:

105039255

MUS:

135626463

ZOF:	121967306 122025747 122025755 122038531

DCT:

110094535

PEQ:

110033088

AOF:

109842617

MSIN:

131256762

ACAK:

137950149

NCOL:

116252457

ATR:

18435495

CJF:

131063774

SMO:	SELMODRAFT_413615 SELMODRAFT_416628

PPP:

112294585

CRE:	CHLRE_06g277200v5

VCN:	VOLCADRAFT_40597

CSL:	COCSUDRAFT_810

CVR:	CHLNCDRAFT_145418

APRO:

F751_0478

OLU:	OSTLU_591 OSTLU_597

OTA:	OT_ostta13g01490

BPG:	Bathy17g01920(DTB)

MIS:	MICPUN_59263

MPP:	MICPUCDRAFT_34980

CME:	CYME_CMG023C

GSL:	Gasu_42400

CCP:	CHC_T00002287001

YLI:	2911900(YALI2_E01336g)

PAN:	PODANSg531

PBEL:

QC761_311150

PPSD:

QC762_311150

PPSP:

QC763_311150

PPSA:

QC764_311150

TTT:	THITE_2058801

MTM:	MYCTH_2299183

SSCK:

SPSK_03105

FGR:	FGSG_09048

FPU:	FPSE_07330

FPOA:

FPOAC1_012695

FVN:	FVRRES_13921

FVR:	FVEG_15894

FOX:	FOXG_19952

NHE:	NECHADRAFT_46109

FFC:	NCS54_00993900

FKR:	NCS57_00988200

FMU:	J7337_009228

TRE:	TRIREDRAFT_65198

TRR:	M419DRAFT_85598

TATV:

25775682(TrAtP1_010389)

MAJ:

MAA_05458

MBRN:

26238989(BIO3-BIO1)

PCHM:

VFPPC_03318

UVI:	66061315(UV8b_00537)

CMT:

CCM_08605

AMUS:

LMH87_000367

PLJ:	28886042(PLICBS_007553)

PTKZ:

JDV02_002469

DCON:

63718180(DCS_05537)

VAL:	VDBG_07632

VDA:	VDAG_09354

CFJ:	CFIO01_05094

CLUP:

CLUP02_08485

CHIG:

CH63R_07606

CDET:

87944812(CDEST_08309)

SAPO:

SAPIO_CDS9256

ELA:	UCREL1_348

PFY:	PFICI_05463

SSL:	SS1G_08439

BFU:	BCIN_12g02430

MBE:

MBM_09317

PSCO:

LY89DRAFT_683081

ANI:	ANIA_06644(biA)

AFM:	AFUA_6G03660

ACT:	ACLA_096920

NFI:	NFIA_050160

AOR:	AO090009000590

ANG:	An15g01990

AFV:	AFLA_010783

ALUC:

AKAW2_60496A

ACHE:

ACHE_50095S

APUU:

APUU_10607S

PCS:	N7525_009721

PDP:	PDIP_16970

POU:	POX_f07633

PPSF:

300788837(PFLUO_LOCUS3145)

TMF:	EYB26_000016

TRG:	TRUGW13939_04272

CIM:	CIMG_01317

CPW:	9691339(D8B26_001861)

URE:	UREG_01244

ABE:

ARB_01948

TVE:

TRV_04743

PBL:	PAAG_08156

PBN:	PADG_08456

AJE:	HCAG_05726

BGH:	BDBG_02033

PNO:	SNOG_15125

PTE:

PTT_18503

PTRR:

6340114(PtrM4_004240)

BZE:	COCCADRAFT_97800

BSC:	COCSADRAFT_40231

BOR:	COCMIDRAFT_95822

AALT:

CC77DRAFT_983495

ADAC:

96084455(ACET3X_004133)

ARAB:

EKO05_0009218

ZTR:	MYCGRDRAFT_111308

PFJ:	MYCFIDRAFT_44846

FFU:	CLAFUR5_12855

CBET:

CB0940_05363

BCOM:

BAUCODRAFT_343702

NPA:	UCRNP2_4321

CNE:

CNB00630

CNB:

CNBB5070

CNG:	CNAG_06739

CGI:	CGB_B9110W

CDEU:

CNBG_6120

CDEC:

89989885(IAS62_003112)

CTEG:

91993633(I308_106780)

CBAS:

91983049(I312_103003)

CDEP:

91088438(L203_104228)

KMG:	30164642(I203_107936)

KNE:	92177833(IAR55_000573)

KDJ:	28967681(I303_103964)

KBI:	30213106(I302_105426)

KPIN:

30169732(I206_103635)

KSH:	87955994(IL334_003863)

KER:	91107347(V865_008546)

KSN:	43592123(CI109_100140)

KEP:	91090782(L201_000110)

TMS:	TREMEDRAFT_30257

CCAC:

CcaHIS019_0508780(bioDA)

TVS:	TRAVEDRAFT_125534

DSQ:	DICSQDRAFT_133242

PCO:	PHACADRAFT_171054

SHS:	STEHIDRAFT_128037

HIR:	HETIRDRAFT_316475

PSQ:	PUNSTDRAFT_92463

ADL:	AURDEDRAFT_100686

FME:	FOMMEDRAFT_102518

GTR:	GLOTRDRAFT_64423

RSX:	RhiXN_02950

LBC:	LACBIDRAFT_190389

CCI:	CC1G_06303

ABP:	AGABI1DRAFT75033(AGABI1DRAFT_75033)

ABV:	AGABI2DRAFT201364(AGABI2DRAFT_201364)

PCUB:

JR316_0006218

MPR:	MPER_02846

MRR:	Moror_4589

SCM:	SCHCO_02625758(SCHCODRAFT_02625758)

CPUT:

CONPUDRAFT_100025

SLA:	SERLADRAFT_438251

WSE:	WALSEDRAFT_52570

WIC:	J056_000827

UMA:	UMAG_02915

PFP:	PFL1_01329

SGRA:

EX895_001765

MGL:

MGL_3585

MRT:

MRET_0544

MSYM:

MSY001_1396

PGR:	PGTG_14186

PTRC:

PtA15_9A440

PSTR:

Pst134EA_021341

MLR:	MELLADRAFT_45909

RIE:	OCT59_029710

PTI:	PHATRDRAFT_19762

PIF:	PITG_06454

PSOJ:

PHYSODRAFT_258968

BLAC:

94344572(CCR75_000795)

SPAR:

SPRG_19814

GTT:	GUITHDRAFT_161318

SBF:	JCM31447_25940

» show all

Reference

1 [PMID:40028677]

Authors

Noble CG, Hollinshead T, Kende A, Langford MP, Lim PP, Linney E, Mattocks J, Swindale LY, Green K.

Title

The plant Diaminopelargonic acid aminotransferase uses spermidine as its amino donor.

Journal

Plant J 121:e70076 (2025)
DOI:10.1111/tpj.70076

Sequence

[ath:AT5G57590]

Other DBs

ExplorEnz - The Enzyme Database:

2.6.1.127

IUBMB Enzyme Nomenclature:

2.6.1.127

ExPASy - ENZYME nomenclature database:

2.6.1.127

BRENDA, the Enzyme Database:

2.6.1.127

LinkDB

REACTION: R13517

Entry

R13517                      Reaction

Name

spermidine:8-amino-7-oxononanoate aminotransferase

Definition

Spermidine + 8-Amino-7-oxononanoate <=> N-(3-Aminopropyl)-4-aminobutanal + 7,8-Diaminononanoate

Equation

C00315 + C01092 <=> C23055 + C01037

Reaction class

RC00887

C01037_C01092

Enzyme

2.6.1.127

Pathway

rn00780

Biotin metabolism

rn01100

Metabolic pathways

Brite

Enzymatic reactions [BR:br08201]
2. Transferase reactions
  2.6  Transferring nitrogenous groups
   2.6.1  Transaminases
    2.6.1.127
     R13517  Spermidine + 8-Amino-7-oxononanoate <=> N-(3-Aminopropyl)-4-aminobutanal + 7,8-Diaminononanoate

Orthology

K19562

bifunctional dethiobiotin synthetase / spermidine---8-amino-7-oxononanoate transaminase [EC:6.3.3.3 2.6.1.127]

LinkDB

DBGET integrated database retrieval system