Requires Mg2+ for maximal activity but can use Mn2+, Fe2+ or Co2+ to a lesser extent . Following on from EC 22.214.171.124, terpentedienyl-diphosphate synthase, this enzyme completes the transformation of geranylgeranyl diphosphate (GGDP) into terpentetriene, which is a precursor of the diterpenoid antibiotic terpentecin. Farnesyl diphosphate can also act as a substrate.
Eguchi T, Dekishima Y, Hamano Y, Dairi T, Seto H, Kakinuma K.
A new approach for the investigation of isoprenoid biosynthesis featuring pathway switching, deuterium hyperlabeling, and 1H NMR spectroscopy. The reaction mechanism of a novel streptomyces diterpene cyclase.