KEGG   ORTHOLOGY: K20502
Entry
K20502            Tight     KO                                     
Symbol
CBDAS
Name
cannabidiolic acid synthase [EC:1.21.3.8]
Pathway
map00999  Biosynthesis of various plant secondary metabolites
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00894  Cannabidiol biosynthesis, malonyl-CoA => cannabidiol/dronabinol
Reaction
R10160  cannabigerolate:oxygen oxidoreductase (cyclizing, cannabidiolate-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00999 Biosynthesis of various plant secondary metabolites
    K20502  CBDAS; cannabidiolic acid synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.21  Catalysing the reaction X-H + Y-H = X-Y
   1.21.3  With oxygen as acceptor
    1.21.3.8  cannabidiolic acid synthase
     K20502  CBDAS; cannabidiolic acid synthase
Genes
CSAV: 115697762
AG: BAF65033(CBDAS)
Reference
PMID:17544411
  Authors
Taura F, Sirikantaramas S, Shoyama Y, Yoshikai K, Shoyama Y, Morimoto S
  Title
Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa.
  Journal
FEBS Lett 581:2929-34 (2007)
DOI:10.1016/j.febslet.2007.05.043
  Sequence
[ag:BAF65033]
LinkDB

KEGG   ENZYME: 1.21.3.8
Entry
EC 1.21.3.8                 Enzyme                                 
Name
cannabidiolic acid synthase;
CBDA synthase
Class
Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With oxygen as acceptor
Sysname
cannabigerolate:oxygen oxidoreductase (cyclizing, cannabidiolate-forming)
Reaction(IUBMB)
cannabigerolate + O2 = cannabidiolate + H2O2 [RN:R10160]
Reaction(KEGG)
R10160
Substrate
cannabigerolate [CPD:C20406];
O2 [CPD:C00007]
Product
cannabidiolate [CPD:C10784];
H2O2 [CPD:C00027]
Comment
Binds FAD covalently. Part of the cannabinoids biosynthetic pathway of the plant Cannabis sativa. The enzyme can also convert cannabinerolate to cannabidiolate with lower efficiency.
History
EC 1.21.3.8 created 2012
Pathway
ec00999  Biosynthesis of various plant secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20502  cannabidiolic acid synthase
Genes
CSAV: 115697762
Reference
1  [PMID:8663284]
  Authors
Taura F, Morimoto S, Shoyama Y
  Title
Purification and characterization of cannabidiolic-acid synthase from Cannabis sativa L.. Biochemical analysis of a novel enzyme that catalyzes the oxidocyclization of cannabigerolic acid to cannabidiolic acid.
  Journal
J Biol Chem 271:17411-6 (1996)
DOI:10.1074/jbc.271.29.17411
  Sequence
[ag:BAF65033]
Reference
2  [PMID:17544411]
  Authors
Taura F, Sirikantaramas S, Shoyama Y, Yoshikai K, Shoyama Y, Morimoto S
  Title
Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa.
  Journal
FEBS Lett 581:2929-34 (2007)
DOI:10.1016/j.febslet.2007.05.043
  Sequence
[ag:BAF65033]
Other DBs
ExplorEnz - The Enzyme Database: 1.21.3.8
IUBMB Enzyme Nomenclature: 1.21.3.8
ExPASy - ENZYME nomenclature database: 1.21.3.8
BRENDA, the Enzyme Database: 1.21.3.8
LinkDB

KEGG   REACTION: R10160
Entry
R10160                      Reaction                               
Name
cannabigerolate:oxygen oxidoreductase (cyclizing, cannabidiolate-forming)
Definition
Cannabigerolate + Oxygen <=> Cannabidiolic acid + Hydrogen peroxide
Equation
C20406 + C00007 <=> C10784 + C00027
Reaction class
RC02755  C00007_C00027
RC03067  C10784_C20406
Enzyme
1.21.3.8
Pathway
rn00999  Biosynthesis of various plant secondary metabolites
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00894  Cannabidiol biosynthesis, malonyl-CoA => cannabidiol/dronabinol
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.21  Catalysing the reaction X-H + Y-H = X-Y
   1.21.3  With oxygen as acceptor
    1.21.3.8
     R10160  Cannabigerolate + Oxygen <=> Cannabidiolic acid + Hydrogen peroxide
Orthology
K20502  cannabidiolic acid synthase [EC:1.21.3.8]
Other DBs
RHEA: 34414
LinkDB

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