KEGG   ORTHOLOGY: K20515
Entry
K20515            Tight     KO                                     
Symbol
PIM1
Name
pimaradiene synthase [EC:4.2.3.147]
Pathway
map00904  Diterpenoid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R06299  (+)-copalyl-diphosphate diphosphate-lyase (pimaradiene-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K20515  PIM1; pimaradiene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.147  pimaradiene synthase
     K20515  PIM1; pimaradiene synthase
Genes
AG: AFU73867(PIM1) AFU73868(PIM1)
Reference
PMID:23370714
  Authors
Hall DE, Zerbe P, Jancsik S, Quesada AL, Dullat H, Madilao LL, Yuen M, Bohlmann J
  Title
Evolution of conifer diterpene synthases: diterpene resin acid biosynthesis in lodgepole pine and jack pine involves monofunctional and bifunctional diterpene synthases.
  Journal
Plant Physiol 161:600-16 (2013)
DOI:10.1104/pp.112.208546
  Sequence
[ag:AFU73867 AFU73868]
LinkDB

KEGG   ENZYME: 4.2.3.147
Entry
EC 4.2.3.147                Enzyme                                 
Name
pimaradiene synthase;
PbmPIM1;
PcmPIM1
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
(+)-copalyl diphosphate-lyase (pimara-8(14),15-diene-forming)
Reaction(IUBMB)
(+)-copalyl diphosphate = pimara-8(14),15-diene + diphosphate [RN:R06299]
Reaction(KEGG)
R06299
Substrate
(+)-copalyl diphosphate [CPD:C11901]
Product
pimara-8(14),15-diene [CPD:C06086];
diphosphate [CPD:C00013]
Comment
Isolated from the plants Pinus banksiana (jack pine) and Pinus contorta (lodgepole pine).
History
EC 4.2.3.147 created 2014
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K20515  pimaradiene synthase
Reference
1  [PMID:23370714]
  Authors
Hall DE, Zerbe P, Jancsik S, Quesada AL, Dullat H, Madilao LL, Yuen M, Bohlmann J
  Title
Evolution of conifer diterpene synthases: diterpene resin acid biosynthesis in lodgepole pine and jack pine involves monofunctional and bifunctional diterpene synthases.
  Journal
Plant Physiol 161:600-16 (2013)
DOI:10.1104/pp.112.208546
  Sequence
[ag:AFU73867 AFU73868]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.147
IUBMB Enzyme Nomenclature: 4.2.3.147
ExPASy - ENZYME nomenclature database: 4.2.3.147
BRENDA, the Enzyme Database: 4.2.3.147
LinkDB

KEGG   REACTION: R06299
Entry
R06299                      Reaction                               
Name
(+)-copalyl-diphosphate diphosphate-lyase (pimaradiene-forming)
Definition
Copalyl diphosphate <=> Pimaradiene + Diphosphate
Equation
C11901 <=> C06086 + C00013
Reaction class
RC01494  C06086_C11901
Enzyme
4.2.3.147
Pathway
rn00904  Diterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.147
     R06299  Copalyl diphosphate <=> Pimaradiene + Diphosphate
Orthology
K20515  pimaradiene synthase [EC:4.2.3.147]
Other DBs
RHEA: 42987
LinkDB

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