KEGG   ORTHOLOGY: K20516
Entry
K20516            Tight     KO                                     
Symbol
CLS
Name
copal-8-ol diphosphate hydratase [EC:4.2.1.133]
Pathway
map00904  Diterpenoid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R09967  geranylgeranyl-diphosphate hydratase (copal-8-ol-diphosphate-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K20516  CLS; copal-8-ol diphosphate hydratase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.133  copal-8-ol diphosphate hydratase
     K20516  CLS; copal-8-ol diphosphate hydratase
Genes
AG: ADJ93862(CLS)
Reference
  Authors
Falara V, Pichersky E, Kanellis AK
  Title
A copal-8-ol diphosphate synthase from the angiosperm Cistus creticus subsp. creticus is a putative key enzyme for the formation of pharmacologically active, oxygen-containing labdane-type diterpenes.
  Journal
Plant Physiol 154:301-10 (2010)
DOI:10.1104/pp.110.159566
  Sequence
LinkDB

KEGG   ORTHOLOGY: K20518
Entry
K20518            Tight     KO                                     
Symbol
CAS
Name
copal-8-ol diphosphate hydratase / cis-abienol synthase [EC:4.2.1.133 4.2.3.140]
Pathway
map00904  Diterpenoid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R09967  geranylgeranyl-diphosphate hydratase (copal-8-ol-diphosphate-forming)
R10276  (13E)-8alpha-hydroxylabd-13-en-15-yl-diphosphate diphosphate-lyase (cis-abienol forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K20518  CAS; copal-8-ol diphosphate hydratase / cis-abienol synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.133  copal-8-ol diphosphate hydratase
     K20518  CAS; copal-8-ol diphosphate hydratase / cis-abienol synthase
   4.2.3  Acting on phosphates
    4.2.3.140  cis-abienol synthase
     K20518  CAS; copal-8-ol diphosphate hydratase / cis-abienol synthase
Genes
AG: AEL99953(CAS)
Reference
  Authors
Zerbe P, Chiang A, Yuen M, Hamberger B, Hamberger B, Draper JA, Britton R, Bohlmann J
  Title
Bifunctional cis-abienol synthase from Abies balsamea discovered by transcriptome sequencing and its implications for diterpenoid fragrance production.
  Journal
J Biol Chem 287:12121-31 (2012)
DOI:10.1074/jbc.M111.317669
  Sequence
LinkDB

KEGG   ENZYME: 4.2.1.133
Entry
EC 4.2.1.133                Enzyme                                 
Name
copal-8-ol diphosphate hydratase;
CcCLS
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
geranylgeranyl-diphosphate hydro-lyase [(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate-forming]
Reaction(IUBMB)
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate = geranylgeranyl diphosphate + H2O [RN:R09967]
Reaction(KEGG)
R09967
Substrate
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate [CPD:C20270]
Product
geranylgeranyl diphosphate [CPD:C00353];
H2O [CPD:C00001]
Comment
Requires Mg2+. The enzyme was characterized from the plant Cistus creticus subsp. creticus.
History
EC 4.2.1.133 created 2012
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K20516  copal-8-ol diphosphate hydratase
K20518  copal-8-ol diphosphate hydratase / cis-abienol synthase
Reference
1  [PMID:20595348]
  Authors
Falara V, Pichersky E, Kanellis AK
  Title
A copal-8-ol diphosphate synthase from the angiosperm Cistus creticus subsp. creticus is a putative key enzyme for the formation of pharmacologically active, oxygen-containing labdane-type diterpenes.
  Journal
Plant Physiol 154:301-10 (2010)
DOI:10.1104/pp.110.159566
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.133
IUBMB Enzyme Nomenclature: 4.2.1.133
ExPASy - ENZYME nomenclature database: 4.2.1.133
BRENDA, the Enzyme Database: 4.2.1.133
LinkDB

KEGG   REACTION: R09967
Entry
R09967                      Reaction                               
Name
geranylgeranyl-diphosphate hydratase (copal-8-ol-diphosphate-forming);
copal-8-ol-diphosphate hydro-lyase
Definition
Copal-8-ol diphosphate <=> Geranylgeranyl diphosphate + H2O
Equation
Reaction class
RC02988  C00353_C20270
Enzyme
Pathway
rn00904  Diterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.133
     R09967  Copal-8-ol diphosphate <=> Geranylgeranyl diphosphate + H2O
Orthology
K20516  copal-8-ol diphosphate hydratase [EC:4.2.1.133]
K20518  copal-8-ol diphosphate hydratase / cis-abienol synthase [EC:4.2.1.133 4.2.3.140]
Other DBs
RHEA: 32706
LinkDB

DBGET integrated database retrieval system