KEGG   ORTHOLOGY: K20621
Entry
K20621            Tight     KO                                     
Symbol
CYP82Y1
Name
(S)-N-methylcanadine 1-hydroxylase [EC:1.14.14.166]
Pathway
map00950  Isoquinoline alkaloid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00946  Noscapine biosynthesis, (S)-reticuline => noscapine
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00950 Isoquinoline alkaloid biosynthesis
    K20621  CYP82Y1; (S)-N-methylcanadine 1-hydroxylase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K20621  CYP82Y1; (S)-N-methylcanadine 1-hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.166  (S)-N-methylcanadine 1-hydroxylase
     K20621  CYP82Y1; (S)-N-methylcanadine 1-hydroxylase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP82 family
   K20621  CYP82Y1; (S)-N-methylcanadine 1-hydroxylase
Other DBs
RN: R11687
Genes
PSOM: 113322396
AG: AFB74617(CYP82Y1)
Reference
  Authors
Dang TT, Facchini PJ
  Title
CYP82Y1 is N-methylcanadine 1-hydroxylase, a key noscapine biosynthetic enzyme in opium poppy.
  Journal
J Biol Chem 289:2013-26 (2014)
DOI:10.1074/jbc.M113.505099
  Sequence
LinkDB

KEGG   ENZYME: 1.14.14.166
Entry
EC 1.14.14.166              Enzyme                                 
Name
(S)-N-methylcanadine 1-hydroxylase;
CYP82Y1 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
(S)-N-methylcanadine,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (1-hydroxylating)
Reaction(IUBMB)
(S)-N-methylcanadine + [reduced NADPH---hemoprotein reductase] + O2 = (S)-1-hydroxy-N-methylcanadine + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11687]
Reaction(KEGG)
R11687
Substrate
(S)-N-methylcanadine [CPD:C02915];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(S)-1-hydroxy-N-methylcanadine [CPD:C21586];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
This cytochrome P-450 (heme-thiolate) enzyme, characterized from the plant Papaver somniferum (opium poppy), participates in the biosynthesis of the isoquinoline alkaloid noscapine.
History
EC 1.14.14.166 created 2018
Pathway
ec00950  Isoquinoline alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20621  (S)-N-methylcanadine 1-hydroxylase
Genes
PSOM113322396
Reference
1  [PMID:24324259]
  Authors
Dang TT, Facchini PJ
  Title
CYP82Y1 is N-methylcanadine 1-hydroxylase, a key noscapine biosynthetic enzyme in opium poppy.
  Journal
J Biol Chem 289:2013-26 (2014)
DOI:10.1074/jbc.M113.505099
  Sequence
Reference
2  [PMID:29610307]
  Authors
Li Y, Li S, Thodey K, Trenchard I, Cravens A, Smolke CD
  Title
Complete biosynthesis of noscapine and halogenated alkaloids in yeast.
  Journal
Proc Natl Acad Sci U S A 115:E3922-E3931 (2018)
DOI:10.1073/pnas.1721469115
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.166
IUBMB Enzyme Nomenclature: 1.14.14.166
ExPASy - ENZYME nomenclature database: 1.14.14.166
BRENDA, the Enzyme Database: 1.14.14.166
LinkDB

KEGG   REACTION: R11687
Entry
R11687                      Reaction                               
Name
(S)-N-methylcanadine,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (1-hydroxylating)
Definition
(S)-N-Methylcanadine + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> (S)-1-Hydroxy-N-methylcanadine + [Oxidized NADPH---hemoprotein reductase] + H2O
Equation
Comment
N-methylcanadine 1-hydroxylase
Reaction class
RC00236  C02915_C21586
Enzyme
Pathway
rn00950  Isoquinoline alkaloid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00946  Noscapine biosynthesis, (S)-reticuline => noscapine
Orthology
K20621  (S)-N-methylcanadine 1-hydroxylase [EC:1.14.14.166]
LinkDB

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