KEGG   ORTHOLOGY: K20944
Entry
K20944            Tight     KO                                     
Symbol
tsdB
Name
resorcinol 4-hydroxylase (NADH) [EC:1.14.13.220]
Pathway
map00362  Benzoate degradation
map01120  Microbial metabolism in diverse environments
Reaction
R11347  resorcinol,NADH:oxygen oxidoreductase (4-hydroxylating)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K20944  tsdB; resorcinol 4-hydroxylase (NADH)
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.220  resorcinol 4-hydroxylase (NADH)
     K20944  tsdB; resorcinol 4-hydroxylase (NADH)
Other DBs
COG: COG0654
Genes
RHA: RHA1_ro01860
RHOJ: JVH1_27990
RHJS: OYT95_29410
Reference
PMID:26319878
  Authors
Kasai D, Araki N, Motoi K, Yoshikawa S, Iino T, Imai S, Masai E, Fukuda M
  Title
gamma-Resorcylate catabolic-pathway genes in the soil actinomycete Rhodococcus jostii RHA1.
  Journal
Appl Environ Microbiol 81:7656-65 (2015)
DOI:10.1128/AEM.02422-15
  Sequence
[rha:RHA1_ro01860]
LinkDB

KEGG   ENZYME: 1.14.13.220
Entry
EC 1.14.13.220              Enzyme                                 
Name
resorcinol 4-hydroxylase (NADH);
tsdB (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
resorcinol,NADH:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
resorcinol + NADH + H+ + O2 = hydroxyquinol + NAD+ + H2O [RN:R11347]
Reaction(KEGG)
R11347
Substrate
resorcinol [CPD:C01751];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
hydroxyquinol [CPD:C02814];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Rhodococcus jostii RHA1, is a single-component hydroxylase. The enzyme has no activity with NADPH. cf. EC 1.14.13.219, resorcinol 4-hydroxylase (NADPH), and EC 1.14.14.27, resorcinol 4-hydroxylase (FADH2).
History
EC 1.14.13.220 created 2016
Pathway
ec00362  Benzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K20944  resorcinol 4-hydroxylase (NADH)
Genes
RHA: RHA1_ro01860
RHOJ: JVH1_27990
RHJS: OYT95_29410
Reference
1  [PMID:26319878]
  Authors
Kasai D, Araki N, Motoi K, Yoshikawa S, Iino T, Imai S, Masai E, Fukuda M
  Title
gamma-Resorcylate catabolic-pathway genes in the soil actinomycete Rhodococcus jostii RHA1.
  Journal
Appl Environ Microbiol 81:7656-65 (2015)
DOI:10.1128/AEM.02422-15
  Sequence
[rha:RHA1_ro01860]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.220
IUBMB Enzyme Nomenclature: 1.14.13.220
ExPASy - ENZYME nomenclature database: 1.14.13.220
BRENDA, the Enzyme Database: 1.14.13.220
LinkDB

KEGG   REACTION: R11347
Entry
R11347                      Reaction                               
Name
resorcinol,NADH:oxygen oxidoreductase (4-hydroxylating)
Definition
Resorcinol + NADH + H+ + Oxygen <=> Benzene-1,2,4-triol + NAD+ + H2O
Equation
C01751 + C00004 + C00080 + C00007 <=> C02814 + C00003 + C00001
Reaction class
RC00046  C01751_C02814
Enzyme
1.14.13.220
Pathway
rn00362  Benzoate degradation
rn01120  Microbial metabolism in diverse environments
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.220
     R11347  Resorcinol + NADH + H+ + Oxygen <=> Benzene-1,2,4-triol + NAD+ + H2O
Orthology
K20944  resorcinol 4-hydroxylase (NADH) [EC:1.14.13.220]
Other DBs
RHEA: 49687
LinkDB

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