KEGG   ORTHOLOGY: K20985
Entry
K20985            Tight     KO                                     
Symbol
LOVG
Name
dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase [EC:3.1.2.31]
Pathway
map00997  Biosynthesis of various other secondary metabolites
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00893  Lovastatin biosynthesis, malonyl-CoA => lovastatin acid
Reaction
R11187  dihydromonacolin L-[acyl-carrier protein] hydrolase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00997 Biosynthesis of various other secondary metabolites
    K20985  LOVG; dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.1  Acting on ester bonds
   3.1.2  Thioester hydrolases
    3.1.2.31  dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase
     K20985  LOVG; dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase
Genes
AG: EAU29411(LOVG)
Reference
PMID:23653178
  Authors
Xu W, Chooi YH, Choi JW, Li S, Vederas JC, Da Silva NA, Tang Y
  Title
LovG: the thioesterase required for dihydromonacolin L release and lovastatin nonaketide synthase turnover in lovastatin biosynthesis.
  Journal
Angew Chem Int Ed Engl 52:6472-5 (2013)
DOI:10.1002/anie.201302406
  Sequence
[ag:EAU29411]
LinkDB

KEGG   ENZYME: 3.1.2.31
Entry
EC 3.1.2.31                 Enzyme                                 
Name
dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase;
LovG
Class
Hydrolases;
Acting on ester bonds;
Thioester hydrolases
Sysname
dihydromonacolin L-[lovastatin nonaketide synthase] hydrolase
Reaction(IUBMB)
dihydromonacolin L-[lovastatin nonaketide synthase] + H2O = holo-[lovastatin nonaketide synthase] + dihydromonacolin L acid
Reaction(KEGG)
(other) R11187
Substrate
dihydromonacolin L-[lovastatin nonaketide synthase];
H2O [CPD:C00001]
Product
holo-[lovastatin nonaketide synthase];
dihydromonacolin L acid [CPD:C20851]
Comment
Dihydromonacolin L acid is synthesized while bound to an acyl-carrier protein domain of the lovastatin nonaketide synthase (EC 2.3.1.161). Since that enzyme lacks a thioesterase domain, release of the dihydromonacolin L acid moiety from the polyketide synthase requires this dedicated enzyme.
History
EC 3.1.2.31 created 2015
Pathway
ec00997  Biosynthesis of various other secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20985  dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase
Reference
1  [PMID:23653178]
  Authors
Xu W, Chooi YH, Choi JW, Li S, Vederas JC, Da Silva NA, Tang Y
  Title
LovG: the thioesterase required for dihydromonacolin L release and lovastatin nonaketide synthase turnover in lovastatin biosynthesis.
  Journal
Angew Chem Int Ed Engl 52:6472-5 (2013)
DOI:10.1002/anie.201302406
  Sequence
[ag:EAU29411]
Other DBs
ExplorEnz - The Enzyme Database: 3.1.2.31
IUBMB Enzyme Nomenclature: 3.1.2.31
ExPASy - ENZYME nomenclature database: 3.1.2.31
BRENDA, the Enzyme Database: 3.1.2.31
LinkDB

KEGG   REACTION: R11187
Entry
R11187                      Reaction                               
Name
dihydromonacolin L-[acyl-carrier protein] hydrolase
Definition
Dihydromonacolin L-[acyl-carrier protein] + H2O <=> Acyl-carrier protein + Dihydromonacolin L acid
Equation
C21108 + C00001 <=> C00229 + C20851
Comment
Acy-carrier protein is acp domain of LOVB [KO:K20981].
Reaction class
RC00014  C20851_C21108
RC00039  C00229_C21108
Enzyme
3.1.2.31
Pathway
rn00997  Biosynthesis of various other secondary metabolites
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00893  Lovastatin biosynthesis, malonyl-CoA => lovastatin acid
Brite
Enzymatic reactions [BR:br08201]
 3. Hydrolase reactions
  3.1  Acting on ester bonds
   3.1.2  Thioester hydrolases
    3.1.2.31
     R11187  Dihydromonacolin L-[acyl-carrier protein] + H2O <=> Acyl-carrier protein + Dihydromonacolin L acid
Orthology
K20985  dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase [EC:3.1.2.31]
Other DBs
RHEA: 11595
LinkDB

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