KEGG   ORTHOLOGY: K21130
Entry
K21130                      KO                                     
Symbol
colC
Name
GDP-L-colitose synthase [EC:1.1.1.356]
Pathway
map00541  O-Antigen nucleotide sugar biosynthesis
map01100  Metabolic pathways
map01250  Biosynthesis of nucleotide sugars
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09107 Glycan biosynthesis and metabolism
   00541 O-Antigen nucleotide sugar biosynthesis
    K21130  colC; GDP-L-colitose synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.356  GDP-L-colitose synthase
     K21130  colC; GDP-L-colitose synthase
Other DBs
RN: R10420 R10421
COG: COG0451
Genes
YSI: BF17_13440
VOG: LCH97_15630
AG: AEP25495(colC)
Reference
  Authors
Alam J, Beyer N, Liu HW
  Title
Biosynthesis of colitose: expression, purification, and mechanistic characterization of GDP-4-keto-6-deoxy-D-mannose-3-dehydrase (ColD) and GDP-L-colitose synthase (ColC).
  Journal
Biochemistry 43:16450-60 (2004)
DOI:10.1021/bi0483763
  Sequence
LinkDB

KEGG   ENZYME: 1.1.1.356
Entry
EC 1.1.1.356                Enzyme                                 
Name
GDP-L-colitose synthase;
ColC
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
GDP-beta-L-colitose:NAD(P)+ 4-oxidoreductase (5-epimerizing)
Reaction(IUBMB)
GDP-beta-L-colitose + NAD(P)+ = GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NAD(P)H + H+ [RN:R10420 R10421]
Reaction(KEGG)
R10420 R10421
Substrate
GDP-beta-L-colitose [CPD:C20613];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose [CPD:C20612];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme is involved in biosynthesis of L-colitose, a 3,6-dideoxyhexose found in the O-antigen of Gram-negative lipopolysaccharides, where it catalyses the reaction in the reverse direction. The enzyme also performs the NAD(P)H-dependent epimerisation at C-5 of the sugar. The enzyme from Yersinia pseudotuberculosis is Si-specific with respect to NAD(P)H [1].
History
EC 1.1.1.356 created 2013
Pathway
ec00541  O-Antigen nucleotide sugar biosynthesis
ec01100  Metabolic pathways
Orthology
K21130  GDP-L-colitose synthase
Genes
YSIBF17_13440
VOGLCH97_15630
Reference
1  [PMID:15610039]
  Authors
Alam J, Beyer N, Liu HW
  Title
Biosynthesis of colitose: expression, purification, and mechanistic characterization of GDP-4-keto-6-deoxy-D-mannose-3-dehydrase (ColD) and GDP-L-colitose synthase (ColC).
  Journal
Biochemistry 43:16450-60 (2004)
DOI:10.1021/bi0483763
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.356
IUBMB Enzyme Nomenclature: 1.1.1.356
ExPASy - ENZYME nomenclature database: 1.1.1.356
BRENDA, the Enzyme Database: 1.1.1.356
LinkDB

KEGG   REACTION: R10420
Entry
R10420                      Reaction                               
Name
GDP-beta-L-colitose:NAD+ 4-oxidoreductase (5-epimerizing)
Definition
GDP-beta-L-colitose + NAD+ <=> GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NADH + H+
Equation
Reaction class
RC00001  C00003_C00004
RC00154  C20612_C20613
Enzyme
Pathway
rn00541  O-Antigen nucleotide sugar biosynthesis
rn01100  Metabolic pathways
rn01250  Biosynthesis of nucleotide sugars
Orthology
K21130  GDP-L-colitose synthase [EC:1.1.1.356]
Other DBs
RHEA: 36570
LinkDB

KEGG   REACTION: R10421
Entry
R10421                      Reaction                               
Name
GDP-beta-L-colitose:NADP+ 4-oxidoreductase (5-epimerizing)
Definition
GDP-beta-L-colitose + NADP+ <=> GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NADPH + H+
Equation
Reaction class
RC00001  C00005_C00006
RC00154  C20612_C20613
Enzyme
Pathway
rn00541  O-Antigen nucleotide sugar biosynthesis
rn01100  Metabolic pathways
rn01250  Biosynthesis of nucleotide sugars
Orthology
K21130  GDP-L-colitose synthase [EC:1.1.1.356]
Other DBs
RHEA: 36566
LinkDB

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