KEGG   ORTHOLOGY: K21146
Entry
K21146                      KO                                     
Symbol
CYP105A1
Name
vitamin D 1,25-hydroxylase [EC:1.14.15.22]
Pathway
map00100  Steroid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Reaction
R11458  calciol,ferredoxin:oxygen oxidoreductase (1,25-hydroxylating)
R11459  
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09103 Lipid metabolism
   00100 Steroid biosynthesis
    K21146  CYP105A1; vitamin D 1,25-hydroxylase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K21146  CYP105A1; vitamin D 1,25-hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.15  With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.15.22  vitamin D 1,25-hydroxylase
     K21146  CYP105A1; vitamin D 1,25-hydroxylase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, bacteria type
  CYP105 family
   K21146  CYP105A1; vitamin D 1,25-hydroxylase
Other DBs
COG: COG2124
Genes
NBR: O3I_016665
NOZ: DMB37_03450
SSX: SACTE_6300
STRE: GZL_08768
SNR: SNOUR_39820
SALU: DC74_467
SALL: SAZ_02815
SGD: ELQ87_03740
SRIM: CP984_00750
SHUN: DWB77_06762
AG: AAA26823(CYP105A1)
 » show all
Reference
PMID:2345149
  Authors
Omer CA, Lenstra R, Litle PJ, Dean C, Tepperman JM, Leto KJ, Romesser JA, O'Keefe DP
  Title
Genes for two herbicide-inducible cytochromes P-450 from Streptomyces griseolus.
  Journal
J Bacteriol 172:3335-45 (1990)
DOI:10.1128/JB.172.6.3335-3345.1990
  Sequence
Reference
  Authors
Sawada N, Sakaki T, Yoneda S, Kusudo T, Shinkyo R, Ohta M, Inouye K
  Title
Conversion of vitamin D3 to 1alpha,25-dihydroxyvitamin D3 by Streptomyces griseolus cytochrome P450SU-1.
  Journal
Biochem Biophys Res Commun 320:156-64 (2004)
DOI:10.1016/j.bbrc.2004.05.140
LinkDB

KEGG   ENZYME: 1.14.15.22
Entry
EC 1.14.15.22               Enzyme                                 
Name
vitamin D 1,25-hydroxylase;
CYP105A1;
Streptomyces griseolus cytochrome P450SU-1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
calciol,ferredoxin:oxygen oxidoreductase (1,25-hydroxylating)
Reaction(IUBMB)
(1) calciol + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = calcidiol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R11458];
(2) calcidiol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = calcitriol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R11459]
Reaction(KEGG)
R11458 R11459
Substrate
calciol [CPD:C05443];
O2 [CPD:C00007];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080];
calcidiol [CPD:C01561]
Product
calcidiol [CPD:C01561];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001];
calcitriol [CPD:C01673]
Comment
A P-450 (heme-thiolate) enzyme found in the bacterium Streptomyces griseolus. cf. EC 1.14.14.24, vitamin D 25-hydroxylase and EC 1.14.15.18, calcidiol 1-monooxygenase.
History
EC 1.14.15.22 created 2016
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K21146  vitamin D 1,25-hydroxylase
Genes
NBRO3I_016665
NOZDMB37_03450
SSXSACTE_6300
STREGZL_08768
SNRSNOUR_39820
SALUDC74_467
SALLSAZ_02815
SGDELQ87_03740
SRIMCP984_00750
SHUNDWB77_06762
SYUNMOV08_40675
SOVQZH56_33105
SLUOPS467_23855
STRHGXP74_28480
SPIQOHA34_14935
NOABKM31_22770
MHAIOHB01_32840 OHB01_32855
 » show all
Reference
1  [PMID:15207715]
  Authors
Sawada N, Sakaki T, Yoneda S, Kusudo T, Shinkyo R, Ohta M, Inouye K
  Title
Conversion of vitamin D3 to 1alpha,25-dihydroxyvitamin D3 by Streptomyces griseolus cytochrome P450SU-1.
  Journal
Biochem Biophys Res Commun 320:156-64 (2004)
DOI:10.1016/j.bbrc.2004.05.140
  Sequence
Reference
2  [PMID:18314962]
  Authors
Sugimoto H, Shinkyo R, Hayashi K, Yoneda S, Yamada M, Kamakura M, Ikushiro S, Shiro Y, Sakaki T
  Title
Crystal structure of CYP105A1 (P450SU-1) in complex with 1alpha,25-dihydroxyvitamin D3.
  Journal
Biochemistry 47:4017-27 (2008)
DOI:10.1021/bi7023767
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.22
IUBMB Enzyme Nomenclature: 1.14.15.22
ExPASy - ENZYME nomenclature database: 1.14.15.22
BRENDA, the Enzyme Database: 1.14.15.22
LinkDB

KEGG   REACTION: R11459
Entry
R11459                      Reaction                               
Definition
Calcidiol + Oxygen + 2 Reduced ferredoxin + 2 H+ <=> Calcitriol + 2 Oxidized ferredoxin + H2O
Equation
Reaction class
RC00723  C01561_C01673
Enzyme
Pathway
rn00100  Steroid biosynthesis
rn01100  Metabolic pathways
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.15  With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.15.22
     R11459  Calcidiol + Oxygen + 2 Reduced ferredoxin + 2 H+ <=> Calcitriol + 2 Oxidized ferredoxin + H2O
Orthology
K21146  vitamin D 1,25-hydroxylase [EC:1.14.15.22]
Other DBs
RHEA: 50703
LinkDB

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