KEGG   ORTHOLOGY: K21724
Entry
K21724                      KO                                     
Symbol
ndmC
Name
7-methylxanthine demethylase [EC:1.14.13.128]
Pathway
map00232  Caffeine metabolism
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
map01120  Microbial metabolism in diverse environments
Module
M00915  Caffeine degradation, caffeine => xanthine
Reaction
R07965  7-methylxanthine:oxygen oxidoreductase (demethylating)
R07966  7-methylxanthine:oxygen oxidoreductase (demethylating)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00232 Caffeine metabolism
    K21724  ndmC; 7-methylxanthine demethylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.128  7-methylxanthine demethylase
     K21724  ndmC; 7-methylxanthine demethylase
Other DBs
COG: COG4638
Genes
PHF: NLY38_11705
PKJ: Q1W70_26420
BFN: OI25_3683
PCAF: DSC91_007652
PTRO: G5S35_35700
PDIO: PDMSB3_2216
PPAI: E1956_28835
MMS: mma_3678
PARH: I5S86_05125
AG: AFD03118(ndmC)
Reference
PMID:22328667
  Authors
Summers RM, Louie TM, Yu CL, Gakhar L, Louie KC, Subramanian M
  Title
Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids.
  Journal
J Bacteriol 194:2041-9 (2012)
DOI:10.1128/JB.06637-11
  Sequence
[ag:AFD03118]
LinkDB

KEGG   ENZYME: 1.14.13.128
Entry
EC 1.14.13.128              Enzyme                                 
Name
7-methylxanthine demethylase;
ndmC (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
7-methylxanthine:oxygen oxidoreductase (demethylating)
Reaction(IUBMB)
7-methylxanthine + O2 + NAD(P)H + H+ = xanthine + NAD(P)+ + H2O + formaldehyde [RN:R07965 R07966]
Reaction(KEGG)
R07965 R07966
Substrate
7-methylxanthine [CPD:C16353];
O2 [CPD:C00007];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Product
xanthine [CPD:C00385];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
formaldehyde [CPD:C00067]
Comment
A non-heme iron oxygenase. The enzyme from the bacterium Pseudomonas putida prefers NADH over NADPH. The enzyme is specific for 7-methylxanthine [2]. Forms part of the caffeine degradation pathway.
History
EC 1.14.13.128 created 2011
Pathway
ec00232  Caffeine metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01120  Microbial metabolism in diverse environments
Orthology
K21724  7-methylxanthine demethylase
Genes
PHF: NLY38_11705
PKJ: Q1W70_26420
BFN: OI25_3683
PCAF: DSC91_007652
PTRO: G5S35_35700
PDIO: PDMSB3_2216
PPAI: E1956_28835
MMS: mma_3678
PARH: I5S86_05125
Reference
1  [PMID:20966097]
  Authors
Summers RM, Louie TM, Yu CL, Subramanian M
  Title
Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source.
  Journal
Microbiology 157:583-92 (2011)
DOI:10.1099/mic.0.043612-0
Reference
2  [PMID:22328667]
  Authors
Summers RM, Louie TM, Yu CL, Gakhar L, Louie KC, Subramanian M
  Title
Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids.
  Journal
J Bacteriol 194:2041-9 (2012)
DOI:10.1128/JB.06637-11
  Sequence
[ag:AFD03118]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.128
IUBMB Enzyme Nomenclature: 1.14.13.128
ExPASy - ENZYME nomenclature database: 1.14.13.128
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.13.128
BRENDA, the Enzyme Database: 1.14.13.128
LinkDB

KEGG   REACTION: R07965
Entry
R07965                      Reaction                               
Name
7-methylxanthine:oxygen oxidoreductase (demethylating)
Definition
7-Methylxanthine + NADH + H+ + Oxygen <=> Xanthine + NAD+ + Formaldehyde + H2O
Equation
C16353 + C00004 + C00080 + C00007 <=> C00385 + C00003 + C00067 + C00001
Reaction class
RC00001  C00003_C00004
RC01797  C00067_C16353
RC01827  C00385_C16353
Enzyme
1.14.13.128
Pathway
rn00232  Caffeine metabolism
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
rn01120  Microbial metabolism in diverse environments
Module
M00915  Caffeine degradation, caffeine => xanthine
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.128
     R07965  7-Methylxanthine + NADH + H+ + Oxygen <=> Xanthine + NAD+ + Formaldehyde + H2O
Orthology
K21724  7-methylxanthine demethylase [EC:1.14.13.128]
Other DBs
RHEA: 30314
LinkDB

KEGG   REACTION: R07966
Entry
R07966                      Reaction                               
Name
7-methylxanthine:oxygen oxidoreductase (demethylating)
Definition
7-Methylxanthine + NADPH + H+ + Oxygen <=> Xanthine + NADP+ + Formaldehyde + H2O
Equation
C16353 + C00005 + C00080 + C00007 <=> C00385 + C00006 + C00067 + C00001
Reaction class
RC00001  C00005_C00006
RC01797  C00067_C16353
RC01827  C00385_C16353
Enzyme
1.14.13.128
Pathway
rn00232  Caffeine metabolism
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
rn01120  Microbial metabolism in diverse environments
Module
M00915  Caffeine degradation, caffeine => xanthine
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.128
     R07966  7-Methylxanthine + NADPH + H+ + Oxygen <=> Xanthine + NADP+ + Formaldehyde + H2O
Orthology
K21724  7-methylxanthine demethylase [EC:1.14.13.128]
Other DBs
RHEA: 30310
LinkDB

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