KEGG   ORTHOLOGY: K21780
Entry
K21780                      KO                                     
Symbol
pigI, redM
Name
L-proline---[L-prolyl-carrier protein] ligase [EC:6.2.1.53]
Pathway
map00333  Prodigiosin biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00837  Prodigiosin biosynthesis, L-proline => prodigiosin
M00838  Undecylprodigiosin biosynthesis, L-proline => undecylprodigiosin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00333 Prodigiosin biosynthesis
    K21780  pigI, redM; L-proline---[L-prolyl-carrier protein] ligase
Enzymes [BR:ko01000]
 6. Ligases
  6.2  Forming carbon-sulfur bonds
   6.2.1  Acid-thiol ligases
    6.2.1.53  L-proline---[L-prolyl-carrier protein] ligase
     K21780  pigI, redM; L-proline---[L-prolyl-carrier protein] ligase
Other DBs
RN: R06746 R12056 R12057
COG: COG1020
Genes
SMW: SMWW4_v1c10960(pigI)
SRR: SerAS9_1745
SRS: SerAS12_1745
SRA: SerAS13_1746
SERF: L085_23070
SERA: Ser39006_020655
SERQ: CWC46_20660
SERM: CLM71_22570
SSUR: ATE40_002195
VGA: BSQ33_00995
VSR: Vspart_01686(pigI)
PSAZ: PA25_35560
HCH: HCH_06032
SCO: SCO5891(redM)
STRD: NI25_09775
AG: CAH55637(pigI)
 » show all
Reference
  Authors
Williamson NR, Simonsen HT, Ahmed RA, Goldet G, Slater H, Woodley L, Leeper FJ, Salmond GP
  Title
Biosynthesis of the red antibiotic, prodigiosin, in Serratia: identification of a novel 2-methyl-3-n-amyl-pyrrole (MAP) assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Streptomyces.
  Journal
Mol Microbiol 56:971-89 (2005)
DOI:10.1111/j.1365-2958.2005.04602.x
  Sequence
Reference
  Authors
Harris AK, Williamson NR, Slater H, Cox A, Abbasi S, Foulds I, Simonsen HT, Leeper FJ, Salmond GP
  Title
The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation.
  Journal
Microbiology 150:3547-60 (2004)
DOI:10.1099/mic.0.27222-0
  Sequence
LinkDB

KEGG   ORTHOLOGY: K21781
Entry
K21781                      KO                                     
Symbol
pigG, redO
Name
peptidyl carrier protein
Pathway
map00333  Prodigiosin biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00837  Prodigiosin biosynthesis, L-proline => prodigiosin
M00838  Undecylprodigiosin biosynthesis, L-proline => undecylprodigiosin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00333 Prodigiosin biosynthesis
    K21781  pigG, redO; peptidyl carrier protein
Other DBs
RN: R06746 R12056 R12057
COG: COG0236
Genes
SMW: SMWW4_v1c10980(pigG)
SRR: SerAS9_1747
SRS: SerAS12_1747
SRA: SerAS13_1748
SERF: L085_23060
SERA: Ser39006_020665
SERQ: CWC46_20670
SERM: CLM71_22580
SSUR: ATE40_002205
VGA: BSQ33_00985
VSR: Vspart_01684(pigG)
PSAZ: PA25_35530
HCH: HCH_06030
SCO: SCO5889(redO)
STRD: NI25_09785
AG: CAH55635(pigG)
 » show all
Reference
  Authors
Williamson NR, Simonsen HT, Ahmed RA, Goldet G, Slater H, Woodley L, Leeper FJ, Salmond GP
  Title
Biosynthesis of the red antibiotic, prodigiosin, in Serratia: identification of a novel 2-methyl-3-n-amyl-pyrrole (MAP) assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Streptomyces.
  Journal
Mol Microbiol 56:971-89 (2005)
DOI:10.1111/j.1365-2958.2005.04602.x
  Sequence
Reference
  Authors
Harris AK, Williamson NR, Slater H, Cox A, Abbasi S, Foulds I, Simonsen HT, Leeper FJ, Salmond GP
  Title
The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation.
  Journal
Microbiology 150:3547-60 (2004)
DOI:10.1099/mic.0.27222-0
  Sequence
LinkDB

KEGG   ENZYME: 6.2.1.53
Entry
EC 6.2.1.53                 Enzyme                                 
Name
L-proline---[L-prolyl-carrier protein] ligase;
pltF (gene name);
bmp4 (gene name);
pigI (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
L-proline:[L-prolyl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-proline + holo-[L-prolyl-carrier protein] = AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein] (overall reaction);
(1a) ATP + L-proline = diphosphate + (L-prolyl)adenylate [RN:R12056];
(1b) (L-prolyl)adenylate + holo-[L-prolyl-carrier protein] = AMP + L-prolyl-[L-prolyl-carrier protein]
Reaction(KEGG)
R12056;
(other) R06746 R12057
Substrate
ATP [CPD:C00002];
L-proline [CPD:C00148];
holo-[L-prolyl-carrier protein];
(L-prolyl)adenylate [CPD:C20656]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-prolyl-[L-prolyl-carrier protein];
(L-prolyl)adenylate [CPD:C20656]
Comment
The enzyme participates in the biosynthesis of several pyrrole-containing compounds, such as undecylprodigiosin, prodigiosin, pyoluteorin, and coumermycin A1. It catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein.
History
EC 6.2.1.53 created 2018
Pathway
ec00333  Prodigiosin biosynthesis
ec00401  Novobiocin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12719  L-proline---[L-prolyl-carrier protein] ligase
K21780  L-proline---[L-prolyl-carrier protein] ligase
Genes
SMWSMWW4_v1c10960(pigI)
SRRSerAS9_1745
SRSSerAS12_1745
SRASerAS13_1746
SERFL085_23070
SERASer39006_020655
SERQCWC46_20660
SERMCLM71_22570
SSURATE40_002195
VGABSQ33_00995
VSRVspart_01686(pigI)
PRRAT705_06620
PTUPTUN_a2940
PVBJ5X90_18250
PSAZPA25_35560
LANTTUM19329_13350
HCHHCH_06032
HAHEENC22_25985
CCUPBKK81_22625
CARIFNU76_06130
KIAG8A07_12235
MDRMDOR_11230
NTPCRH09_26890
NAHF5544_12760
SCOSCO5891(redM)
SLVSLIV_09150
SGMGCM10017557_78140 GCM10017557_82750
STRDNI25_09775
SSIAA7J05_21360
SALFSMD44_07462
SRKFGW37_26810
SPADDVK44_30970
SRIMCP984_39845
SSPBCP982_34450
SFEUIM697_30930
ARUBJ5A65_13345
NGVCDO52_00495
PSUUPsuf_018720
CATICS0771_15580
 » show all
Reference
1  [PMID:11880032]
  Authors
Thomas MG, Burkart MD, Walsh CT.
  Title
Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis.
  Journal
Chem Biol 9:171-84 (2002)
DOI:10.1016/S1074-5521(02)00100-X
Reference
2  [PMID:15528645]
  Authors
Harris AK, Williamson NR, Slater H, Cox A, Abbasi S, Foulds I, Simonsen HT, Leeper FJ, Salmond GP
  Title
The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation.
  Journal
Microbiology 150:3547-60 (2004)
DOI:10.1099/mic.0.27222-0
  Sequence
Reference
3  [PMID:15853884]
  Authors
Williamson NR, Simonsen HT, Ahmed RA, Goldet G, Slater H, Woodley L, Leeper FJ, Salmond GP
  Title
Biosynthesis of the red antibiotic, prodigiosin, in Serratia: identification of a novel 2-methyl-3-n-amyl-pyrrole (MAP) assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Streptomyces.
  Journal
Mol Microbiol 56:971-89 (2005)
DOI:10.1111/j.1365-2958.2005.04602.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.53
IUBMB Enzyme Nomenclature: 6.2.1.53
ExPASy - ENZYME nomenclature database: 6.2.1.53
BRENDA, the Enzyme Database: 6.2.1.53
LinkDB

KEGG   REACTION: R06746
Entry
R06746                      Reaction                               
Name
L-proline:[peptidyl-carrier protein] ligase (AMP-forming)
Definition
L-Proline + Peptidyl-carrier protein + ATP <=> L-Prolyl-[pcp] + AMP + Diphosphate
Equation
Comment
two-step reaction (see R12056+R12057)
Reaction class
RC00039  C12470_C21097
RC01438  C00148_C12470
Enzyme
Pathway
rn00333  Prodigiosin biosynthesis
rn00401  Novobiocin biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00837  Prodigiosin biosynthesis, L-proline => prodigiosin
M00838  Undecylprodigiosin biosynthesis, L-proline => undecylprodigiosin
Orthology
K12719  L-proline---[L-prolyl-carrier protein] ligase [EC:6.2.1.53]
K12720  peptidyl carrier protein
K21780  L-proline---[L-prolyl-carrier protein] ligase [EC:6.2.1.53]
K21781  peptidyl carrier protein
LinkDB

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