KEGG   ORTHOLOGY: K22097
Entry
K22097                      KO                                     
Symbol
CXE1
Name
3-O-acetylpapaveroxine carboxylesterase [EC:3.1.1.105]
Pathway
map00950  Isoquinoline alkaloid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00946  Noscapine biosynthesis, (S)-reticuline => noscapine
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00950 Isoquinoline alkaloid biosynthesis
    K22097  CXE1; 3-O-acetylpapaveroxine carboxylesterase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.1  Acting on ester bonds
   3.1.1  Carboxylic-ester hydrolases
    3.1.1.105  3-O-acetylpapaveroxine carboxylesterase
     K22097  CXE1; 3-O-acetylpapaveroxine carboxylesterase
Other DBs
RN: R11693 R11694
Genes
PSOM: 113322812 113323289
AG: AFB74618(CXE1)
Reference
  Authors
Winzer T, Gazda V, He Z, Kaminski F, Kern M, Larson TR, Li Y, Meade F, Teodor R, Vaistij FE, Walker C, Bowser TA, Graham IA
  Title
A Papaver somniferum 10-gene cluster for synthesis of the anticancer alkaloid noscapine.
  Journal
Science 336:1704-8 (2012)
DOI:10.1126/science.1220757
  Sequence
Reference
  Authors
Dang TT, Chen X, Facchini PJ
  Title
Acetylation serves as a protective group in noscapine biosynthesis in opium poppy.
  Journal
Nat Chem Biol 11:104-6 (2015)
DOI:10.1038/nchembio.1717
LinkDB

KEGG   ENZYME: 3.1.1.105
Entry
EC 3.1.1.105                Enzyme                                 
Name
3-O-acetylpapaveroxine carboxylesterase;
CXE1 (gene name)
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
Sysname
3-O-acetylpapaveroxine acetatehydrolase
Reaction(IUBMB)
3-O-acetylpapaveroxine + H2O = narcotine hemiacetal + acetate [RN:R12347]
Reaction(KEGG)
R12347;
(other) R11693 R11694
Substrate
3-O-acetylpapaveroxine [CPD:C21591];
H2O [CPD:C00001]
Product
narcotine hemiacetal [CPD:C20297];
acetate [CPD:C00033]
Comment
The enzyme, characterized from the plant Papaver somniferum (opium poppy), participates in the biosynthesis of the isoquinoline alkaloid noscapine.
History
EC 3.1.1.105 created 2019
Pathway
ec00950  Isoquinoline alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K22097  3-O-acetylpapaveroxine carboxylesterase
Genes
PSOM113322812 113323289
Reference
1  [PMID:25485687]
  Authors
Dang TT, Chen X, Facchini PJ
  Title
Acetylation serves as a protective group in noscapine biosynthesis in opium poppy.
  Journal
Nat Chem Biol 11:104-6 (2015)
DOI:10.1038/nchembio.1717
Reference
2  [PMID:29723437]
  Authors
Park MR, Chen X, Lang DE, Ng KKS, Facchini PJ
  Title
Heterodimeric O-methyltransferases involved in the biosynthesis of noscapine in opium poppy.
  Journal
Plant J 95:252-267 (2018)
DOI:10.1111/tpj.13947
Other DBs
ExplorEnz - The Enzyme Database: 3.1.1.105
IUBMB Enzyme Nomenclature: 3.1.1.105
ExPASy - ENZYME nomenclature database: 3.1.1.105
BRENDA, the Enzyme Database: 3.1.1.105
LinkDB

KEGG   REACTION: R11693
Entry
R11693                      Reaction                               
Name
3-O-acetylpapaveroxine acetatehydrolase
Definition
3-O-Acetylpapaveroxine + H2O <=> Papaveroxine + Acetate
Equation
Reaction class
RC00037  C20299_C21591
Enzyme
Pathway
rn00950  Isoquinoline alkaloid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00946  Noscapine biosynthesis, (S)-reticuline => noscapine
Orthology
K22097  3-O-acetylpapaveroxine carboxylesterase [EC:3.1.1.105]
LinkDB

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