KEGG   ORTHOLOGY: K22269
Entry
K22269            Tight     KO                                     
Symbol
rosA
Name
8-amino-8-demethylriboflavin N,N-dimethyltransferase [EC:2.1.1.343]
Pathway
map00998  Biosynthesis of various antibiotics
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00890  Roseoflavin biosynthesis, FMN => roseoflavin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00998 Biosynthesis of various antibiotics
    K22269  rosA; 8-amino-8-demethylriboflavin N,N-dimethyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.343  8-amino-8-demethylriboflavin N,N-dimethyltransferase
     K22269  rosA; 8-amino-8-demethylriboflavin N,N-dimethyltransferase
Other DBs
RN: R11778 R11779 R11780
Genes
SDV: BN159_8032(rosA)
Reference
  Authors
Jankowitsch F, Kuhm C, Kellner R, Kalinowski J, Pelzer S, Macheroux P, Mack M
  Title
A novel N,N-8-amino-8-demethyl-D-riboflavin Dimethyltransferase (RosA) catalyzing the two terminal steps of roseoflavin biosynthesis in Streptomyces davawensis.
  Journal
J Biol Chem 286:38275-85 (2011)
DOI:10.1074/jbc.M111.292300
  Sequence
Reference
  Authors
Tongsook C, Uhl MK, Jankowitsch F, Mack M, Gruber K, Macheroux P
  Title
Structural and kinetic studies on RosA, the enzyme catalysing the methylation of 8-demethyl-8-amino-d-riboflavin to the antibiotic roseoflavin.
  Journal
FEBS J 283:1531-49 (2016)
DOI:10.1111/febs.13690
  Sequence
LinkDB

KEGG   ENZYME: 2.1.1.343
Entry
EC 2.1.1.343                Enzyme                                 
Name
8-amino-8-demethylriboflavin N,N-dimethyltransferase;
rosA (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:8-amino-8-demethylriboflavin N,N-dimethyltransferase
Reaction(IUBMB)
2 S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = 2 S-adenosyl-L-homocysteine + roseoflavin (overall reaction) [RN:R11778];
(1a) S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = S-adenosyl-L-homocysteine + 8-demethyl-8-(methylamino)riboflavin [RN:R11779];
(1b) S-adenosyl-L-methionine + 8-demethyl-8-(methylamino)riboflavin = S-adenosyl-L-homocysteine + roseoflavin [RN:R11780]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
8-amino-8-demethylriboflavin [CPD:C21646];
8-demethyl-8-(methylamino)riboflavin [CPD:C21648]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
roseoflavin [CPD:C21647];
8-demethyl-8-(methylamino)riboflavin [CPD:C21648]
Comment
The enzyme, characterized from the soil bacterium Streptomyces davawensis, catalyses the last two steps in the biosynthesis of the antibiotic roseoflavin.
History
EC 2.1.1.343 created 2017
Pathway
ec00998  Biosynthesis of various antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K22269  8-amino-8-demethylriboflavin N,N-dimethyltransferase
Genes
SDVBN159_8032(rosA)
Reference
1  [PMID:21911488]
  Authors
Jankowitsch F, Kuhm C, Kellner R, Kalinowski J, Pelzer S, Macheroux P, Mack M
  Title
A novel N,N-8-amino-8-demethyl-D-riboflavin Dimethyltransferase (RosA) catalyzing the two terminal steps of roseoflavin biosynthesis in Streptomyces davawensis.
  Journal
J Biol Chem 286:38275-85 (2011)
DOI:10.1074/jbc.M111.292300
  Sequence
Reference
2  [PMID:26913589]
  Authors
Tongsook C, Uhl MK, Jankowitsch F, Mack M, Gruber K, Macheroux P
  Title
Structural and kinetic studies on RosA, the enzyme catalysing the methylation of 8-demethyl-8-amino-d-riboflavin to the antibiotic roseoflavin.
  Journal
FEBS J 283:1531-49 (2016)
DOI:10.1111/febs.13690
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.343
IUBMB Enzyme Nomenclature: 2.1.1.343
ExPASy - ENZYME nomenclature database: 2.1.1.343
BRENDA, the Enzyme Database: 2.1.1.343
LinkDB

KEGG   REACTION: R11779
Entry
R11779                      Reaction                               
Name
S-adenosyl-L-methionine:8-amino-8-demethylriboflavin N-methyltransferase
Definition
S-Adenosyl-L-methionine + 8-Amino-8-demethylriboflavin <=> S-Adenosyl-L-homocysteine + 8-Demethyl-8-(methylamino)riboflavin
Equation
Comment
first step of two-step reaction (see R11778, R11779+R11780)
subsequently R11780
Reaction class
RC00003  C00019_C00021
RC00335  C21646_C21648
Enzyme
Pathway
rn00998  Biosynthesis of various antibiotics
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00890  Roseoflavin biosynthesis, FMN => roseoflavin
Orthology
K22269  8-amino-8-demethylriboflavin N,N-dimethyltransferase [EC:2.1.1.343]
Other DBs
RHEA: 53419
LinkDB

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