Entry Name 8-amino-8-demethylriboflavin N,N-dimethyltransferase;
Class Transferases;BRITE hierarchy
Sysname S-adenosyl-L-methionine:8-amino-8-demethylriboflavin N,N-dimethyltransferase
Reaction(IUBMB) 2 S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = 2 S-adenosyl-L-homocysteine + roseoflavin (overall reaction) [RN:
R11778 ];
(1a) S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = S-adenosyl-L-homocysteine + 8-demethyl-8-(methylamino)riboflavin [RN:
R11779 ];
(1b) S-adenosyl-L-methionine + 8-demethyl-8-(methylamino)riboflavin = S-adenosyl-L-homocysteine + roseoflavin [RN:
R11780 ]
Reaction(KEGG) Substrate S-adenosyl-L-methionine [CPD:
C00019 ];
8-amino-8-demethylriboflavin [CPD:
C21646 ];
8-demethyl-8-(methylamino)riboflavin [CPD:
C21648 ]
Product S-adenosyl-L-homocysteine [CPD:
C00021 ];
roseoflavin [CPD:
C21647 ];
8-demethyl-8-(methylamino)riboflavin [CPD:
C21648 ]
Comment The enzyme, characterized from the soil bacterium Streptomyces davawensis, catalyses the last two steps in the biosynthesis of the antibiotic roseoflavin.
History EC 2.1.1.343 created 2017
Pathway ec00998 Biosynthesis of various antibiotics
ec01110 Biosynthesis of secondary metabolites
Orthology K22269 8-amino-8-demethylriboflavin N,N-dimethyltransferase
Genes Taxonomy Reference Authors Jankowitsch F, Kuhm C, Kellner R, Kalinowski J, Pelzer S, Macheroux P, Mack M
Title A novel N,N-8-amino-8-demethyl-D-riboflavin Dimethyltransferase (RosA) catalyzing the two terminal steps of roseoflavin biosynthesis in Streptomyces davawensis.
Journal Sequence Reference Authors Tongsook C, Uhl MK, Jankowitsch F, Mack M, Gruber K, Macheroux P
Title Structural and kinetic studies on RosA, the enzyme catalysing the methylation of 8-demethyl-8-amino-d-riboflavin to the antibiotic roseoflavin.
Journal Sequence Other DBs ExPASy - ENZYME nomenclature database: 2.1.1.343
LinkDB All DBs
