KEGG   ORTHOLOGY: K22638
Entry
K22638            Tight     KO                                     
Symbol
CYP76AK6
Name
carnosic acid synthase [EC:1.14.14.61]
Pathway
map00904  Diterpenoid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R11973  11-hydroxyferruginol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase
R11989  ferruginol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K22638  CYP76AK6; carnosic acid synthase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K22638  CYP76AK6; carnosic acid synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.61  carnosic acid synthase
     K22638  CYP76AK6; carnosic acid synthase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP76 family
   K22638  CYP76AK6; carnosic acid synthase
Genes
AG: ALM25797(CYP76AK6) AOW42544(CYP76AK6)
Reference
PMID:27703160
  Authors
Scheler U, Brandt W, Porzel A, Rothe K, Manzano D, Bozic D, Papaefthimiou D, Balcke GU, Henning A, Lohse S, Marillonnet S, Kanellis AK, Ferrer A, Tissier A
  Title
Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast.
  Journal
Nat Commun 7:12942 (2016)
DOI:10.1038/ncomms12942
  Sequence
[ag:AOW42544]
Reference
PMID:26976595
  Authors
Ignea C, Athanasakoglou A, Ioannou E, Georgantea P, Trikka FA, Loupassaki S, Roussis V, Makris AM, Kampranis SC
  Title
Carnosic acid biosynthesis elucidated by a synthetic biology platform.
  Journal
Proc Natl Acad Sci U S A 113:3681-6 (2016)
DOI:10.1073/pnas.1523787113
  Sequence
[ag:ALM25797]
LinkDB

KEGG   ENZYME: 1.14.14.61
Entry
EC 1.14.14.61               Enzyme                                 
Name
carnosic acid synthase;
CYP76AK6;
CYP76AK7;
CYP76AK8
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
11-hydroxyferruginol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase
Reaction(IUBMB)
11-hydroxyferruginol + 3 [reduced NADPH---hemoprotein reductase] + 3 O2 = carnosic acid + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O [RN:R11973]
Reaction(KEGG)
R11973;
(other) R11989
Substrate
11-hydroxyferruginol [CPD:C21796];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
carnosic acid [CPD:C21818];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from the plants Salvia pomifera (apple sage), S. miltiorrhiza (red sage), S. fruticosa (Greek sage) and Rosmarinus officinalis (Rosemary).
History
EC 1.14.14.61 created 2018
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K22638  carnosic acid synthase
Reference
1  [PMID:26976595]
  Authors
Ignea C, Athanasakoglou A, Ioannou E, Georgantea P, Trikka FA, Loupassaki S, Roussis V, Makris AM, Kampranis SC
  Title
Carnosic acid biosynthesis elucidated by a synthetic biology platform.
  Journal
Proc Natl Acad Sci U S A 113:3681-6 (2016)
DOI:10.1073/pnas.1523787113
  Sequence
[ag:ALM25797]
Reference
2  [PMID:27703160]
  Authors
Scheler U, Brandt W, Porzel A, Rothe K, Manzano D, Bozic D, Papaefthimiou D, Balcke GU, Henning A, Lohse S, Marillonnet S, Kanellis AK, Ferrer A, Tissier A
  Title
Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast.
  Journal
Nat Commun 7:12942 (2016)
DOI:10.1038/ncomms12942
  Sequence
[ag:AOW42544]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.61
IUBMB Enzyme Nomenclature: 1.14.14.61
ExPASy - ENZYME nomenclature database: 1.14.14.61
BRENDA, the Enzyme Database: 1.14.14.61
LinkDB

KEGG   REACTION: R11973
Entry
R11973                      Reaction                               
Name
11-hydroxyferruginol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase
Definition
11-Hydroxyferruginol + 3 [Reduced NADPH---hemoprotein reductase] + 3 Oxygen <=> Carnosic acid + 3 [Oxidized NADPH---hemoprotein reductase] + 4 H2O
Equation
C21796 + 3 C03024 + 3 C00007 <=> C21818 + 3 C03161 + 4 C00001
Reaction class
RC01952  C21796_C21818
Enzyme
1.14.14.61
Pathway
rn00904  Diterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.61
     R11973  11-Hydroxyferruginol + 3 [Reduced NADPH---hemoprotein reductase] + 3 Oxygen <=> Carnosic acid + 3 [Oxidized NADPH---hemoprotein reductase] + 4 H2O
Orthology
K22638  carnosic acid synthase [EC:1.14.14.61]
Other DBs
RHEA: 55435
LinkDB

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