KEGG   ORTHOLOGY: K22945
Entry
K22945            Tight     KO                                     
Symbol
TPS2
Name
nezukol synthase [EC:4.2.3.183]
Pathway
map00904  Diterpenoid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R11848  (+)-copalyl-diphosphate diphosphate-lyase (cyclizing, nezukol-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K22945  TPS2; nezukol synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.183  nezukol synthase
     K22945  TPS2; nezukol synthase
Genes
AG: ARO38140(TPS2)
Reference
PMID:28445526
  Authors
Pelot KA, Hagelthorn DM, Addison JB, Zerbe P
  Title
Biosynthesis of the oxygenated diterpene nezukol in the medicinal plant Isodon rubescens is catalyzed by a pair of diterpene synthases.
  Journal
PLoS One 12:e0176507 (2017)
DOI:10.1371/journal.pone.0176507
  Sequence
[ag:ARO38140]
LinkDB

KEGG   ENZYME: 4.2.3.183
Entry
EC 4.2.3.183                Enzyme                                 
Name
nezukol synthase;
TPS2
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
(+)-copalyl-diphosphate diphosphate-lyase (cyclizing, nezukol-forming)
Reaction(IUBMB)
(+)-copalyl diphosphate + H2O = nezukol + diphosphate [RN:R11848]
Reaction(KEGG)
R11848
Substrate
(+)-copalyl diphosphate [CPD:C11901];
H2O [CPD:C00001]
Product
nezukol [CPD:C21709];
diphosphate [CPD:C00013]
Comment
Isolated from the plant Isodon rubescens.
History
EC 4.2.3.183 created 2017
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K22945  nezukol synthase
Reference
1  [PMID:28445526]
  Authors
Pelot KA, Hagelthorn DM, Addison JB, Zerbe P
  Title
Biosynthesis of the oxygenated diterpene nezukol in the medicinal plant Isodon rubescens is catalyzed by a pair of diterpene synthases.
  Journal
PLoS One 12:e0176507 (2017)
DOI:10.1371/journal.pone.0176507
  Sequence
[ag:ARO38140]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.183
IUBMB Enzyme Nomenclature: 4.2.3.183
ExPASy - ENZYME nomenclature database: 4.2.3.183
BRENDA, the Enzyme Database: 4.2.3.183
LinkDB

KEGG   REACTION: R11848
Entry
R11848                      Reaction                               
Name
(+)-copalyl-diphosphate diphosphate-lyase (cyclizing, nezukol-forming)
Definition
Copalyl diphosphate + H2O <=> Nezukol + Diphosphate
Equation
C11901 + C00001 <=> C21709 + C00013
Enzyme
4.2.3.183
Pathway
rn00904  Diterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.183
     R11848  Copalyl diphosphate + H2O <=> Nezukol + Diphosphate
Orthology
K22945  nezukol synthase [EC:4.2.3.183]
Other DBs
RHEA: 54379
LinkDB

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