KEGG   ORTHOLOGY: K22997
Entry
K22997                      KO                                     
Symbol
cotB3
Name
cyclooctat-9-en-7-ol 5-monooxygenase [EC:1.14.99.61]
Pathway
map00997  Biosynthesis of various other secondary metabolites
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00921  Cyclooctatin biosynthesis, dimethylallyl-PP + isopentenyl-PP => cyclooctatin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00997 Biosynthesis of various other secondary metabolites
    K22997  cotB3; cyclooctat-9-en-7-ol 5-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.99  Miscellaneous
    1.14.99.61  cyclooctat-9-en-7-ol 5-monooxygenase
     K22997  cotB3; cyclooctat-9-en-7-ol 5-monooxygenase
Other DBs
RN: R12177
COG: COG2124
Genes
PAUU: E8A73_024010
NGP: LTT66_22745
SVL: Strvi_5780
SRC: M271_43270
SMAL: SMALA_0743
SSOI: I1A49_07305
SCI: B446_28655
SRN: A4G23_04518(ptlI_2)
SAUO: BV401_07565
SBAT: G4Z16_02340
SHUN: DWB77_00118(ptlI_1) DWB77_06794(ptlI_3)
AG: BAI44339(cotB3)
 » show all
Reference
  Authors
Kim SY, Zhao P, Igarashi M, Sawa R, Tomita T, Nishiyama M, Kuzuyama T
  Title
Cloning and heterologous expression of the cyclooctatin biosynthetic gene cluster afford a diterpene cyclase and two p450 hydroxylases.
  Journal
Chem Biol 16:736-43 (2009)
DOI:10.1016/j.chembiol.2009.06.007
  Sequence
Reference
  Authors
Gorner C, Schrepfer P, Redai V, Wallrapp F, Loll B, Eisenreich W, Haslbeck M, Bruck T
  Title
Identification, characterization and molecular adaptation of class I redox systems for the production of hydroxylated diterpenoids.
  Journal
Microb Cell Fact 15:86 (2016)
DOI:10.1186/s12934-016-0487-6
LinkDB

KEGG   ENZYME: 1.14.99.61
Entry
EC 1.14.99.61               Enzyme                                 
Name
cyclooctat-9-en-7-ol 5-monooxygenase;
CotB3
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
cyclooctat-9-en-7-ol,hydrogen-donor:oxygen oxidoreductase (5-hydroxylating)
Reaction(IUBMB)
cyclooctat-9-en-7-ol + reduced acceptor + O2 = cyclooctat-9-ene-5,7-diol + acceptor + H2O [RN:R12177]
Reaction(KEGG)
R12177
Substrate
cyclooctat-9-en-7-ol [CPD:C20834];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
cyclooctat-9-ene-5,7-diol [CPD:C21978];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
Isolated from the bacterium Streptomyces melanosporofaciens M1614-43f2. Involved in the biosynthesis of cyclooctatin.
History
EC 1.14.99.61 created 2018
Pathway
ec00997  Biosynthesis of various other secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K22997  cyclooctat-9-en-7-ol 5-monooxygenase
Genes
PAUUE8A73_024010
NGPLTT66_22745
SVLStrvi_5780
SRCM271_43270
SMALSMALA_0743
SSOII1A49_07305
SCIB446_28655
SRNA4G23_04518(ptlI_2)
SAUOBV401_07565
SBATG4Z16_02340
SHUNDWB77_00118(ptlI_1) DWB77_06794(ptlI_3)
SFBCP974_24300
 » show all
Reference
1  [PMID:19635410]
  Authors
Kim SY, Zhao P, Igarashi M, Sawa R, Tomita T, Nishiyama M, Kuzuyama T
  Title
Cloning and heterologous expression of the cyclooctatin biosynthetic gene cluster afford a diterpene cyclase and two p450 hydroxylases.
  Journal
Chem Biol 16:736-43 (2009)
DOI:10.1016/j.chembiol.2009.06.007
  Sequence
Reference
2  [PMID:27216162]
  Authors
Gorner C, Schrepfer P, Redai V, Wallrapp F, Loll B, Eisenreich W, Haslbeck M, Bruck T
  Title
Identification, characterization and molecular adaptation of class I redox systems for the production of hydroxylated diterpenoids.
  Journal
Microb Cell Fact 15:86 (2016)
DOI:10.1186/s12934-016-0487-6
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.61
IUBMB Enzyme Nomenclature: 1.14.99.61
ExPASy - ENZYME nomenclature database: 1.14.99.61
BRENDA, the Enzyme Database: 1.14.99.61
LinkDB

KEGG   REACTION: R12177
Entry
R12177                      Reaction                               
Name
cyclooctat-9-en-7-ol,hydrogen-donor:oxygen oxidoreductase (5-hydroxylating)
Definition
Cyclooctat-9-en-7-ol + Reduced acceptor + Oxygen <=> Cyclooctat-9-ene-5,7-diol + Acceptor + H2O
Equation
Reaction class
RC00661  C20834_C21978
Enzyme
Pathway
rn00997  Biosynthesis of various other secondary metabolites
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00921  Cyclooctatin biosynthesis, dimethylallyl-PP + isopentenyl-PP => cyclooctatin
Orthology
K22997  cyclooctat-9-en-7-ol 5-monooxygenase [EC:1.14.99.61]
Other DBs
RHEA: 56823
LinkDB

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