KEGG ORTHOLOGY: K22997

ORTHOLOGY: K22997

Entry

K22997                      KO

Symbol

cotB3

Name

cyclooctat-9-en-7-ol 5-monooxygenase [EC:1.14.99.61]

Pathway

map00997

Biosynthesis of various other secondary metabolites

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Module

M00921

Cyclooctatin biosynthesis, dimethylallyl-PP + isopentenyl-PP => cyclooctatin

Reaction

R12177

cyclooctat-9-en-7-ol,hydrogen-donor:oxygen oxidoreductase (5-hydroxylating)

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00997 Biosynthesis of various other secondary metabolites
    K22997  cotB3; cyclooctat-9-en-7-ol 5-monooxygenase
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.99  Miscellaneous
    1.14.99.61  cyclooctat-9-en-7-ol 5-monooxygenase
     K22997  cotB3; cyclooctat-9-en-7-ol 5-monooxygenase

Other DBs

COG:

COG2124

Genes

PAUU:

E8A73_024010

NGP:	LTT66_22745

SVL:	Strvi_5780

SRC:	M271_43270

SMAL:

SMALA_0743

SSOI:

I1A49_07305

SCI:	B446_28655

SRN:	A4G23_04518(ptlI_2)

SAUO:

BV401_07565

SBAT:

G4Z16_02340

SHUN:

DWB77_00118(ptlI_1) DWB77_06794(ptlI_3)

SFB:	CP974_24300

SGOB:

test1122_24930

SANT:

QR300_36890

SOV:	QZH56_30190

SPRF:

K4G22_25295

SLUO:

PS467_06930

STRH:

GXP74_22405

NRN:	SHK17_18500

KUT:	JJ691_12260

MCHL:

PVK74_00305

AG:	BAI44339(cotB3)

» show all

Reference

PMID:19635410

Authors

Kim SY, Zhao P, Igarashi M, Sawa R, Tomita T, Nishiyama M, Kuzuyama T

Title

Cloning and heterologous expression of the cyclooctatin biosynthetic gene cluster afford a diterpene cyclase and two p450 hydroxylases.

Journal

Chem Biol 16:736-43 (2009)
DOI:10.1016/j.chembiol.2009.06.007

Sequence

[ag:BAI44339]

Reference

PMID:27216162

Authors

Gorner C, Schrepfer P, Redai V, Wallrapp F, Loll B, Eisenreich W, Haslbeck M, Bruck T

Title

Identification, characterization and molecular adaptation of class I redox systems for the production of hydroxylated diterpenoids.

Journal

Microb Cell Fact 15:86 (2016)
DOI:10.1186/s12934-016-0487-6

LinkDB

ENZYME: 1.14.99.61

Entry

EC 1.14.99.61               Enzyme

Name

cyclooctat-9-en-7-ol 5-monooxygenase;
CotB3

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous

Sysname

cyclooctat-9-en-7-ol,hydrogen-donor:oxygen oxidoreductase (5-hydroxylating)

Reaction(IUBMB)

cyclooctat-9-en-7-ol + reduced acceptor + O2 = cyclooctat-9-ene-5,7-diol + acceptor + H2O [RN:R12177]

Reaction(KEGG)

R12177

Substrate

cyclooctat-9-en-7-ol [CPD:C20834];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]

Product

cyclooctat-9-ene-5,7-diol [CPD:C21978];
acceptor [CPD:C00028];
H2O [CPD:C00001]

Comment

Isolated from the bacterium Streptomyces melanosporofaciens M1614-43f2. Involved in the biosynthesis of cyclooctatin.

History

EC 1.14.99.61 created 2018

Pathway

ec00997

Biosynthesis of various other secondary metabolites

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K22997

cyclooctat-9-en-7-ol 5-monooxygenase

Genes

PAUU:

E8A73_024010

NGP:	LTT66_22745

SVL:	Strvi_5780

SRC:	M271_43270

SMAL:

SMALA_0743

SSOI:

I1A49_07305

SCI:	B446_28655

SRN:	A4G23_04518(ptlI_2)

SAUO:

BV401_07565

SBAT:

G4Z16_02340

SHUN:

DWB77_00118(ptlI_1) DWB77_06794(ptlI_3)

SFB:	CP974_24300

SGOB:

test1122_24930

SANT:

QR300_36890

SOV:	QZH56_30190

SPRF:

K4G22_25295

SLUO:

PS467_06930

STRH:

GXP74_22405

NRN:	SHK17_18500

KUT:	JJ691_12260

MCHL:

PVK74_00305

» show all

Reference

1 [PMID:19635410]

Authors

Kim SY, Zhao P, Igarashi M, Sawa R, Tomita T, Nishiyama M, Kuzuyama T

Title

Cloning and heterologous expression of the cyclooctatin biosynthetic gene cluster afford a diterpene cyclase and two p450 hydroxylases.

Journal

Chem Biol 16:736-43 (2009)
DOI:10.1016/j.chembiol.2009.06.007

Sequence

[ag:BAI44339]

Reference

2 [PMID:27216162]

Authors

Gorner C, Schrepfer P, Redai V, Wallrapp F, Loll B, Eisenreich W, Haslbeck M, Bruck T

Title

Identification, characterization and molecular adaptation of class I redox systems for the production of hydroxylated diterpenoids.

Journal

Microb Cell Fact 15:86 (2016)
DOI:10.1186/s12934-016-0487-6

Other DBs

ExplorEnz - The Enzyme Database:

1.14.99.61

IUBMB Enzyme Nomenclature:

1.14.99.61

ExPASy - ENZYME nomenclature database:

1.14.99.61

BRENDA, the Enzyme Database:

1.14.99.61

LinkDB

REACTION: R12177

Entry

R12177                      Reaction

Name

cyclooctat-9-en-7-ol,hydrogen-donor:oxygen oxidoreductase (5-hydroxylating)

Definition

Cyclooctat-9-en-7-ol + Reduced acceptor + Oxygen <=> Cyclooctat-9-ene-5,7-diol + Acceptor + H2O

Equation

C20834 + C00030 + C00007 <=> C21978 + C00028 + C00001

Reaction class

RC00661

C20834_C21978

Enzyme

1.14.99.61

Pathway

rn00997

Biosynthesis of various other secondary metabolites

rn01100

Metabolic pathways

rn01110

Biosynthesis of secondary metabolites

Module

M00921

Cyclooctatin biosynthesis, dimethylallyl-PP + isopentenyl-PP => cyclooctatin

Brite

Enzymatic reactions [BR:br08201]
1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.99  Miscellaneous
    1.14.99.61
     R12177  Cyclooctat-9-en-7-ol + Reduced acceptor + Oxygen <=> Cyclooctat-9-ene-5,7-diol + Acceptor + H2O

Orthology

K22997

cyclooctat-9-en-7-ol 5-monooxygenase [EC:1.14.99.61]

Other DBs

RHEA:

56823

LinkDB

DBGET integrated database retrieval system