KEGG   ORTHOLOGY: K22998
Entry
K22998                      KO                                     
Symbol
cotB4
Name
cyclooctatin synthase [EC:1.14.99.62]
Pathway
map00997  Biosynthesis of various other secondary metabolites
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00921  Cyclooctatin biosynthesis, dimethylallyl-PP + isopentenyl-PP => cyclooctatin
Reaction
R12178  cyclooctat-9-ene-5,7-diol,hydrogen-donor:oxygen oxidoreductase (18-hydroxylating)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00997 Biosynthesis of various other secondary metabolites
    K22998  cotB4; cyclooctatin synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.99  Miscellaneous
    1.14.99.62  cyclooctatin synthase
     K22998  cotB4; cyclooctatin synthase
Other DBs
COG: COG2124
Genes
SVL: Strvi_5781
SRC: M271_43265
SMAL: SMALA_0744
SSOI: I1A49_07310
SCI: B446_28650
SRN: A4G23_04519(ptlI_3)
SAUO: BV401_07570
SFB: CP974_24305
SCAE: IHE65_00210
SXT: KPP03845_200298(ptlI)
SANT: QR300_36885
SOV: QZH56_08935 QZH56_30195
SLUO: PS467_06935
AG: BAI44340(cotB4)
 » show all
Reference
PMID:19635410
  Authors
Kim SY, Zhao P, Igarashi M, Sawa R, Tomita T, Nishiyama M, Kuzuyama T
  Title
Cloning and heterologous expression of the cyclooctatin biosynthetic gene cluster afford a diterpene cyclase and two p450 hydroxylases.
  Journal
Chem Biol 16:736-43 (2009)
DOI:10.1016/j.chembiol.2009.06.007
  Sequence
[ag:BAI44340]
Reference
PMID:27216162
  Authors
Gorner C, Schrepfer P, Redai V, Wallrapp F, Loll B, Eisenreich W, Haslbeck M, Bruck T
  Title
Identification, characterization and molecular adaptation of class I redox systems for the production of hydroxylated diterpenoids.
  Journal
Microb Cell Fact 15:86 (2016)
DOI:10.1186/s12934-016-0487-6
LinkDB

KEGG   ENZYME: 1.14.99.62
Entry
EC 1.14.99.62               Enzyme                                 
Name
cyclooctatin synthase;
CotB4
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
cyclooctat-9-ene-5,7-diol,hydrogen-donor:oxygen oxidoreductase (18-hydroxylating)
Reaction(IUBMB)
cyclooctat-9-ene-5,7-diol + reduced acceptor + O2 = cyclooctatin + acceptor + H2O [RN:R12178]
Reaction(KEGG)
R12178
Substrate
cyclooctat-9-ene-5,7-diol [CPD:C21978];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
cyclooctatin [CPD:C21979];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
Isolated from the bacterium Streptomyces melanosporofaciens M1614-43f2.
History
EC 1.14.99.62 created 2018
Pathway
ec00997  Biosynthesis of various other secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K22998  cyclooctatin synthase
Genes
SVL: Strvi_5781
SRC: M271_43265
SMAL: SMALA_0744
SSOI: I1A49_07310
SCI: B446_28650
SRN: A4G23_04519(ptlI_3)
SAUO: BV401_07570
SFB: CP974_24305
SCAE: IHE65_00210
SXT: KPP03845_200298(ptlI)
SANT: QR300_36885
SOV: QZH56_08935 QZH56_30195
SLUO: PS467_06935
 » show all
Reference
1  [PMID:19635410]
  Authors
Kim SY, Zhao P, Igarashi M, Sawa R, Tomita T, Nishiyama M, Kuzuyama T
  Title
Cloning and heterologous expression of the cyclooctatin biosynthetic gene cluster afford a diterpene cyclase and two p450 hydroxylases.
  Journal
Chem Biol 16:736-43 (2009)
DOI:10.1016/j.chembiol.2009.06.007
  Sequence
[ag:BAI44340]
Reference
2  [PMID:27216162]
  Authors
Gorner C, Schrepfer P, Redai V, Wallrapp F, Loll B, Eisenreich W, Haslbeck M, Bruck T
  Title
Identification, characterization and molecular adaptation of class I redox systems for the production of hydroxylated diterpenoids.
  Journal
Microb Cell Fact 15:86 (2016)
DOI:10.1186/s12934-016-0487-6
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.62
IUBMB Enzyme Nomenclature: 1.14.99.62
ExPASy - ENZYME nomenclature database: 1.14.99.62
BRENDA, the Enzyme Database: 1.14.99.62
LinkDB

KEGG   REACTION: R12178
Entry
R12178                      Reaction                               
Name
cyclooctat-9-ene-5,7-diol,hydrogen-donor:oxygen oxidoreductase (18-hydroxylating)
Definition
Cyclooctat-9-ene-5,7-diol + Reduced acceptor + Oxygen <=> Cyclooctatin + Acceptor + H2O
Equation
C21978 + C00030 + C00007 <=> C21979 + C00028 + C00001
Reaction class
RC03228  C21978_C21979
Enzyme
1.14.99.62
Pathway
rn00997  Biosynthesis of various other secondary metabolites
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00921  Cyclooctatin biosynthesis, dimethylallyl-PP + isopentenyl-PP => cyclooctatin
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.99  Miscellaneous
    1.14.99.62
     R12178  Cyclooctat-9-ene-5,7-diol + Reduced acceptor + Oxygen <=> Cyclooctatin + Acceptor + H2O
Orthology
K22998  cyclooctatin synthase [EC:1.14.99.62]
Other DBs
RHEA: 56827
LinkDB

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