KEGG   ORTHOLOGY: K23121
Entry
K23121                      KO                                     
Symbol
pflD
Name
trans-4-hydroxy-L-proline dehydratase [EC:4.2.1.172]
Pathway
map00330  Arginine and proline metabolism
map01100  Metabolic pathways
Reaction
R11819  trans-4-hydroxy-L-proline hydro-lyase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00330 Arginine and proline metabolism
    K23121  pflD; trans-4-hydroxy-L-proline dehydratase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.172  trans-4-hydroxy-L-proline dehydratase
     K23121  pflD; trans-4-hydroxy-L-proline dehydratase
Other DBs
COG: COG1882
Genes
HMR: Hipma_0362
DAV: DESACE_04125
GSU: GSU2101
GSK: KN400_2127
GEB: GM18_2359
GEV: GSVR_10040
GEC: GEO60473_15450
GBM: Gbem_2499
GBN: GEOBRER4_17200
GER: KP004_08265
GSUB: KP001_06890
GNT: KP003_07795
GPL: M1B72_12535
PSYF: N1030_14415
DSB: LN040_10005
DBA: Dbac_2366
DESU: NLA06_01160
DPS: DP0616(pflD)
DLM: DPPLL_00240
DAT: HRM2_04250(pflD1) HRM2_49330(pflD2)
DOV: DSCO28_52190
DWD: DSCW_54540
DALK: DSCA_46900
DLI: dnl_30770
DEK: DSLASN_43020
AMUC: Pan181_08370(bssA)
PBU: L21SP3_00214(csdB)
PBP: STSP1_00751(csdB)
ALUS: STSP2_03489(bssA)
GEP: Q9293_04450
MSIL: METEAL_32670
MSEA: METESE_10340
BVU: BVU_1421
BDO: EL88_01945
BDH: GV66_04255
PSAC: PSM36_1174
PDI: BDI_0786
PARC: CI960_07850
PMER: INE87_00975(pflD)
PGOL: K6V26_10100
PJO: LK449_08315
PFAI: NXY11_09110
PABC: NEE14_002080(hypD)
OSP: Odosp_3648
PCOU: L6468_02095
TTZ: FHG85_12835
BLQ: L21SP5_00958(bssA)
DORI: FH5T_08275
DRC: G0Q07_01065
MCOS: GM418_11240
ANF: AQPE_2112
MBAS: ALGA_0749
ATK: AUTU_04410
OCI: FEZ18_06875
SHYR: LA303_00540
CPO: COPRO5265_1565
DDF: DEFDS_2103
DAP: Dacet_1516
GTL: EP073_08865
EGV: EGCR1_16435
EAV: EH197_03950
ERAF: J9537_19250
EDE: AAEO79_16295(hypD)
VLU: M2919_03875
CBO: CBO3183
CBA: CLB_3219
CBH: CLC_3093
CBY: CLM_3595
CBL: CLK_2581
CBB: CLD_1348
CBI: CLJ_B3455
CBF: CLI_3322
CBM: CBF_3314
CBJ: H04402_03270
CKL: CKL_0323
CKR: CKR_0275
CSQ: CSCA_0866
CLD: CLSPO_c32860(pflD2)
CACE: CACET_c25680(pflD2)
CCK: Ccar_09825
CTYK: CTK_C05150
CFM: BJL90_19455
CARG: RSJ17_07625
CDRK: B9W14_14245
CFER: D4Z93_05160
CCAA: KQH81_14215
CIU: G4D55_13890
CLOO: RCG18_16905
CCAK: ACFLKC_15090(hypD)
HHW: NCTC503_02347(csdB_2)
CPRF: K7H06_19485
CRAS: KVH43_07795
OCW: OW730_15950
OVA: OBV_40980
CAML: H6X83_10710
DOR: Desor_3978
DAI: Desaci_1175
DMI: Desmer_3087
DRM: Dred_2965
DRU: Desru_0509
DFG: B0537_08335
FWA: DCMF_25705
ACAE: HYG86_12305
CTHM: CFE_0574
PROM: QO263_00140
BPRM: CL3_28280
CCOM: I6K69_04740
BYL: A4V09_06310
BPRO: PMF13cell1_04098(cutC_3)
BCOC: BLCOC_31170(pflD)
BLAR: LC508_03850
CSCI: HDCHBGLK_00253(cutC_1)
CHYL: CE91St63_26530(pflD_2)
CSO: CLS_03180
DLO: K5I24_04245
SINT: K5I26_05885
SCAG: ACI3DM_03555(hypD)
SCAZ: ACI3DN_07435(hypD)
VGU: HYG85_21430
MDV: C5Q96_03640
MNL: QU661_05190
EMJ: V1225_13465(hypD)
AMT: Amet_0584
GFE: Gferi_16240
PBIF: KXZ80_10205
PSOR: RSJ16_12040
CDF: CD630_32820(pflD)
PDC: CDIF630_03582
CDC: CD196_3097(pflD)
CDL: CDR20291_3143(pflD)
PDF: CD630DERM_32820(pflD)
PHX: KGNDJEFE_00506(cutC_2)
TEM: JW646_00595
TGC: L0P85_07750
TMY: TEMA_14950(pflD)
TPET: TPHSE_16750(pflD)
TVT: TVTCOM_23960(pflD)
TEB: T8CH_1917(pflD)
THYR: P4S50_08750
TEP: TepRe1_0044
TAE: TepiRe1_0046
BACG: D2962_11640
NTH: Nther_2476
KME: H0A61_00345(cutC)
SPOK: ACFWUA_07855
SEDZ: JYG23_11695
SEDM: RBQ61_00360
PUF: UFO1_0399
PFT: JBW_04226
MANA: MAMMFC1_02761(bssA_3)
STED: SPTER_40410(cutC_6)
SSPH: SPSPH_042650(pflD_2)
SACV: SPACI_007320(pflD_1) SPACI_054540(pflD_5)
SCAO: SCACP_08860(pflD_2)
AARG: Aargi30884_27260(pflD_2)
ABSI: A9CBEGH2_00460(pflD_1)
ERB: A4V01_16645
BSUE: BS3272_00540
BAQK: QN215_07590
TAI: Taci_1437
CPOR: BED41_02450
TLI: Tlie_1574
AMO: Anamo_1952
ARAM: KAR29_12355
APRP: KIH16_12540
TLE: Tlet_1141
OCS: OF820_05755
CSOM: MKD34_09420
TKO: TK0289
TSI: TSIB_0631
TLT: OCC_00382
ABI: Aboo_0169
ACF: AciM339_0879
KCR: Kcr_0517
LOB: NEF87_002435
PSYT: DSAG12_03102(hypD)
 » show all
Reference
PMID:28183913
  Authors
Levin BJ, Huang YY, Peck SC, Wei Y, Martinez-Del Campo A, Marks JA, Franzosa EA, Huttenhower C, Balskus EP
  Title
A prominent glycyl radical enzyme in human gut microbiomes metabolizes trans-4-hydroxy-l-proline.
  Journal
Science 355:eaai8386 (2017)
DOI:10.1126/science.aai8386
  Sequence
[cdf:CD630_32820]
LinkDB

KEGG   ENZYME: 4.2.1.172
Entry
EC 4.2.1.172                Enzyme                                 
Name
trans-4-hydroxy-L-proline dehydratase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
trans-4-hydroxy-L-proline hydro-lyase
Reaction(IUBMB)
trans-4-hydroxy-L-proline = (S)-1-pyrroline-5-carboxylate + H2O [RN:R11819]
Reaction(KEGG)
R11819
Substrate
trans-4-hydroxy-L-proline [CPD:C01157]
Product
(S)-1-pyrroline-5-carboxylate [CPD:C03912];
H2O [CPD:C00001]
Comment
The enzyme has been characterized from the bacterium Peptoclostridium difficile. The active form contains a glycyl radical that is generated by a dedicated activating enzyme via chemistry involving S-adenosyl-L-methionine (SAM) and a [4Fe-4S] cluster.
History
EC 4.2.1.172 created 2017
Pathway
ec00330  Arginine and proline metabolism
ec01100  Metabolic pathways
Orthology
K23121  trans-4-hydroxy-L-proline dehydratase
Genes
HMR: Hipma_0362
DAV: DESACE_04125
GSU: GSU2101
GSK: KN400_2127
GEB: GM18_2359
GEV: GSVR_10040
GEC: GEO60473_15450
GBM: Gbem_2499
GBN: GEOBRER4_17200
GER: KP004_08265
GSUB: KP001_06890
GNT: KP003_07795
GPL: M1B72_12535
PSYF: N1030_14415
DSB: LN040_10005
DBA: Dbac_2366
DESU: NLA06_01160
DPS: DP0616(pflD)
DLM: DPPLL_00240
DAT: HRM2_04250(pflD1) HRM2_49330(pflD2)
DOV: DSCO28_52190
DWD: DSCW_54540
DALK: DSCA_46900
DLI: dnl_30770
DEK: DSLASN_43020
AMUC: Pan181_08370(bssA)
PBU: L21SP3_00214(csdB)
PBP: STSP1_00751(csdB)
ALUS: STSP2_03489(bssA)
GEP: Q9293_04450
MSIL: METEAL_32670
MSEA: METESE_10340
BVU: BVU_1421
BDO: EL88_01945
BDH: GV66_04255
PSAC: PSM36_1174
PDI: BDI_0786
PARC: CI960_07850
PMER: INE87_00975(pflD)
PGOL: K6V26_10100
PJO: LK449_08315
PFAI: NXY11_09110
PABC: NEE14_002080(hypD)
OSP: Odosp_3648
PCOU: L6468_02095
TTZ: FHG85_12835
BLQ: L21SP5_00958(bssA)
DORI: FH5T_08275
DRC: G0Q07_01065
MCOS: GM418_11240
ANF: AQPE_2112
MBAS: ALGA_0749
ATK: AUTU_04410
OCI: FEZ18_06875
SHYR: LA303_00540
CPO: COPRO5265_1565
DDF: DEFDS_2103
DAP: Dacet_1516
GTL: EP073_08865
EGV: EGCR1_16435
EAV: EH197_03950
ERAF: J9537_19250
EDE: AAEO79_16295(hypD)
VLU: M2919_03875
CBO: CBO3183
CBA: CLB_3219
CBH: CLC_3093
CBY: CLM_3595
CBL: CLK_2581
CBB: CLD_1348
CBI: CLJ_B3455
CBF: CLI_3322
CBM: CBF_3314
CBJ: H04402_03270
CKL: CKL_0323
CKR: CKR_0275
CSQ: CSCA_0866
CLD: CLSPO_c32860(pflD2)
CACE: CACET_c25680(pflD2)
CCK: Ccar_09825
CTYK: CTK_C05150
CFM: BJL90_19455
CARG: RSJ17_07625
CDRK: B9W14_14245
CFER: D4Z93_05160
CCAA: KQH81_14215
CIU: G4D55_13890
CLOO: RCG18_16905
CCAK: ACFLKC_15090(hypD)
HHW: NCTC503_02347(csdB_2)
CPRF: K7H06_19485
CRAS: KVH43_07795
OCW: OW730_15950
OVA: OBV_40980
CAML: H6X83_10710
DOR: Desor_3978
DAI: Desaci_1175
DMI: Desmer_3087
DRM: Dred_2965
DRU: Desru_0509
DFG: B0537_08335
FWA: DCMF_25705
ACAE: HYG86_12305
CTHM: CFE_0574
PROM: QO263_00140
BPRM: CL3_28280
CCOM: I6K69_04740
BYL: A4V09_06310
BPRO: PMF13cell1_04098(cutC_3)
BCOC: BLCOC_31170(pflD)
BLAR: LC508_03850
CSCI: HDCHBGLK_00253(cutC_1)
CHYL: CE91St63_26530(pflD_2)
CSO: CLS_03180
DLO: K5I24_04245
SINT: K5I26_05885
SCAG: ACI3DM_03555(hypD)
SCAZ: ACI3DN_07435(hypD)
VGU: HYG85_21430
MDV: C5Q96_03640
MNL: QU661_05190
EMJ: V1225_13465(hypD)
AMT: Amet_0584
GFE: Gferi_16240
PBIF: KXZ80_10205
PSOR: RSJ16_12040
CDF: CD630_32820(pflD)
PDC: CDIF630_03582
CDC: CD196_3097(pflD)
CDL: CDR20291_3143(pflD)
PDF: CD630DERM_32820(pflD)
PHX: KGNDJEFE_00506(cutC_2)
TEM: JW646_00595
TGC: L0P85_07750
TMY: TEMA_14950(pflD)
TPET: TPHSE_16750(pflD)
TVT: TVTCOM_23960(pflD)
TEB: T8CH_1917(pflD)
THYR: P4S50_08750
TEP: TepRe1_0044
TAE: TepiRe1_0046
BACG: D2962_11640
NTH: Nther_2476
KME: H0A61_00345(cutC)
SPOK: ACFWUA_07855
SEDZ: JYG23_11695
SEDM: RBQ61_00360
PUF: UFO1_0399
PFT: JBW_04226
MANA: MAMMFC1_02761(bssA_3)
STED: SPTER_40410(cutC_6)
SSPH: SPSPH_042650(pflD_2)
SACV: SPACI_007320(pflD_1) SPACI_054540(pflD_5)
SCAO: SCACP_08860(pflD_2)
AARG: Aargi30884_27260(pflD_2)
ABSI: A9CBEGH2_00460(pflD_1)
ERB: A4V01_16645
BSUE: BS3272_00540
BAQK: QN215_07590
TAI: Taci_1437
CPOR: BED41_02450
TLI: Tlie_1574
AMO: Anamo_1952
ARAM: KAR29_12355
APRP: KIH16_12540
TLE: Tlet_1141
OCS: OF820_05755
CSOM: MKD34_09420
TKO: TK0289
TSI: TSIB_0631
TLT: OCC_00382
ABI: Aboo_0169
ACF: AciM339_0879
KCR: Kcr_0517
LOB: NEF87_002435
PSYT: DSAG12_03102(hypD)
 » show all
Reference
1  [PMID:28183913]
  Authors
Levin BJ, Huang YY, Peck SC, Wei Y, Martinez-Del Campo A, Marks JA, Franzosa EA, Huttenhower C, Balskus EP
  Title
A prominent glycyl radical enzyme in human gut microbiomes metabolizes trans-4-hydroxy-l-proline.
  Journal
Science 355:eaai8386 (2017)
DOI:10.1126/science.aai8386
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.172
IUBMB Enzyme Nomenclature: 4.2.1.172
ExPASy - ENZYME nomenclature database: 4.2.1.172
BRENDA, the Enzyme Database: 4.2.1.172
LinkDB

KEGG   REACTION: R11819
Entry
R11819                      Reaction                               
Name
trans-4-hydroxy-L-proline hydro-lyase
Definition
Hydroxyproline <=> (S)-1-Pyrroline-5-carboxylate + H2O
Equation
C01157 <=> C03912 + C00001
Reaction class
RC03501  C01157_C03912
Enzyme
4.2.1.172
Pathway
rn00330  Arginine and proline metabolism
rn01100  Metabolic pathways
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.172
     R11819  Hydroxyproline <=> (S)-1-Pyrroline-5-carboxylate + H2O
Orthology
K23121  trans-4-hydroxy-L-proline dehydratase [EC:4.2.1.172]
Other DBs
RHEA: 54647
LinkDB

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