KEGG   ORTHOLOGY: K23372
Entry
K23372                      KO                                     
Symbol
sbnE
Name
L-2,3-diaminopropanoate---citrate ligase [EC:6.3.2.54]
Pathway
map00997  Biosynthesis of various other secondary metabolites
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00875  Staphyloferrin B biosynthesis, L-serine => staphyloferrin B
Reaction
R12308  L-2,3-diaminopropanoate:citrate ligase (AMP, 2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00997 Biosynthesis of various other secondary metabolites
    K23372  sbnE; L-2,3-diaminopropanoate---citrate ligase
Enzymes [BR:ko01000]
 6. Ligases
  6.3  Forming carbon-nitrogen bonds
   6.3.2  Acid-D-amino-acid ligases (peptide synthases)
    6.3.2.54  L-2,3-diaminopropanoate---citrate ligase
     K23372  sbnE; L-2,3-diaminopropanoate---citrate ligase
Other DBs
COG: COG4264
Genes
HSV: HNO53_08390
RSO: RSp0421
RSE: F504_3903(sbnE)
RPU: CDC45_19735
RNC: GO999_24075
RSL: RPSI07_mp0382
RSN: RSPO_m01407
RSM: CMR15_mp10434
RSY: RSUY_34890(iucA)
RSG: JK151_14165
CNC: CNE_2c14870
CUH: BJN34_08305
RME: Rmet_1113
CUK: KB879_12575
BPLA: bpln_2g05050
NLC: EBAPG3_006470
NIZ: NNRS527_01546
ALEG: CFBP4996_27400
MIWA: SS37A_35510(sbnE_1) SS37A_39090(sbnE_2)
SBD: ATN00_04610
BBAD: K7T73_21440
PCAL: BV455_03920(iucA)
LGY: T479_22390
SALE: EPH95_16125
SAU: SA0116(sbnE)
SAV: SAV0120
SAW: SAHV_0119
SAH: SaurJH1_0111
SAJ: SaurJH9_0107
SAM: MW0093(sbnE)
SAS: SAS0094
SAR: SAR0123
SAC: SACOL0104
SAX: USA300HOU_0131
SAA: SAUSA300_0122
SAO: SAOUHSC_00079
SAE: NWMN_0064(sbnE)
SAD: SAAV_0088
SUU: M013TW_0110(sbnE)
SUV: SAVC_00340
SUE: SAOV_0067
SUJ: SAA6159_00103(sbnE)
SUK: SAA6008_00098(sbnE)
SUC: ECTR2_76
SUT: SAT0131_00106
SUQ: HMPREF0772_10377
SUZ: MS7_0112
SUD: ST398NM01_0132
SUX: SAEMRSA15_00860
SUW: SATW20_01320(sbnE)
SUG: SAPIG0132
SUF: SARLGA251_00950(sbnE)
SAUA: SAAG_00604
SAUE: RSAU_000074
SAUN: SAKOR_00094
SAUS: SA40_0087(sbnE)
SAUU: SA957_0102(sbnE)
SAUG: SA268_0099(sbnE)
SAUZ: SAZ172_0131(sbnE)
SAUT: SAI1T1_2000760
SAUJ: SAI2T2_1000760
SAUK: SAI3T3_1000760
SAUQ: SAI4T8_1000760
SAUV: SAI7S6_1000760
SAUW: SAI5S5_1000760
SAUX: SAI6T6_1000760
SAUY: SAI8T7_1000760
SAUF: X998_0099
SAB: SAB0059
SUY: SA2981_0121(sbnE)
SAUB: C248_0108
SAUM: BN843_1220
SAUC: CA347_131
SAUR: SABB_01720(sbnE)
SAUI: AZ30_00625
SAUD: CH52_05120
SAMS: NI36_00520
SUH: SAMSHR1132_00960
SSD: SPSINT_0338
SDT: SPSE_2120
SDP: NCTC12225_02337(iucA)
SHU: SHYC_00375(sbnE)
SSCH: LH95_00375
SSCZ: RN70_00595
SAGQ: EP23_09750
SEQO: SE1039_25070
SLZ: B5P37_07610
SARL: SAP2_02070
SSH: NCTC13712_00073(iucA_1)
SRAI: LN051_00250
SFF: FOB90_07415
BAGR: BA6348_15335
BHUI: LOK74_15440
BBOR: RFB14_00525
PPOL: X809_04485
PTA: HPL003_19610
PPEO: ABE82_04640
PNP: IJ22_24510
POW: IJ21_46100
PXL: BS614_08680
PKB: B4V02_14045
PAIH: ASL14_00120
PPSC: EHS13_31660
PBAC: HUB98_21830
PKA: PQ456_10715
PMAH: PTQ21_17775
PMAE: LMZ02_21545
ANX: ACH33_02505
ATHE: K3F53_16445
SACA: FFV09_03670
TAB: CIG75_16795
LFB: C1X05_04250
KPUL: GXN76_14215
PABS: JIR001_05130(sbnE)
SACG: FDZ84_26450
PMAD: BAY61_28585
 » show all
Reference
PMID:14688077
  Authors
Dale SE, Doherty-Kirby A, Lajoie G, Heinrichs DE
  Title
Role of siderophore biosynthesis in virulence of Staphylococcus aureus: identification and characterization of genes involved in production of a siderophore.
  Journal
Infect Immun 72:29-37 (2004)
DOI:10.1128/IAI.72.1.29-37.2004
  Sequence
[sam:MW0093]
LinkDB

KEGG   ENZYME: 6.3.2.54
Entry
EC 6.3.2.54                 Enzyme                                 
Name
L-2,3-diaminopropanoate---citrate ligase;
sbnE (gene name);
2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate synthtase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
Sysname
L-2,3-diaminopropanoate:citrate ligase (2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate-forming)
Reaction(IUBMB)
ATP + L-2,3-diaminopropanoate + citrate = AMP + diphosphate + 2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate [RN:R12308]
Reaction(KEGG)
R12308
Substrate
ATP [CPD:C00002];
L-2,3-diaminopropanoate [CPD:C03401];
citrate [CPD:C00158]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate [CPD:C22072]
Comment
Requires Mg2+. The enzyme, characterized from the bacterium Staphylococcus aureus, is involved in the biosynthesis of the siderophore staphyloferrin B. It belongs to a class of siderophore synthases known as type A nonribosomal peptide synthase-independent synthases (NIS). Type A NIS enzymes are responsible for the formation of amide or ester bonds between polyamines or amino alcohols and a prochiral carboxyl group of citrate. The enzyme forms a citrate adenylate intermediate prior to ligation.
History
EC 6.3.2.54 created 2019
Pathway
ec00997  Biosynthesis of various other secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K23372  L-2,3-diaminopropanoate---citrate ligase
Genes
HSV: HNO53_08390
RSO: RSp0421
RSE: F504_3903(sbnE)
RPU: CDC45_19735
RNC: GO999_24075
RSL: RPSI07_mp0382
RSN: RSPO_m01407
RSM: CMR15_mp10434
RSY: RSUY_34890(iucA)
RSG: JK151_14165
CNC: CNE_2c14870
CUH: BJN34_08305
RME: Rmet_1113
CUK: KB879_12575
BPLA: bpln_2g05050
NLC: EBAPG3_006470
NIZ: NNRS527_01546
ALEG: CFBP4996_27400
MIWA: SS37A_35510(sbnE_1) SS37A_39090(sbnE_2)
SBD: ATN00_04610
BBAD: K7T73_21440
PCAL: BV455_03920(iucA)
LGY: T479_22390
SALE: EPH95_16125
SAU: SA0116(sbnE)
SAV: SAV0120
SAW: SAHV_0119
SAH: SaurJH1_0111
SAJ: SaurJH9_0107
SAM: MW0093(sbnE)
SAS: SAS0094
SAR: SAR0123
SAC: SACOL0104
SAX: USA300HOU_0131
SAA: SAUSA300_0122
SAO: SAOUHSC_00079
SAE: NWMN_0064(sbnE)
SAD: SAAV_0088
SUU: M013TW_0110(sbnE)
SUV: SAVC_00340
SUE: SAOV_0067
SUJ: SAA6159_00103(sbnE)
SUK: SAA6008_00098(sbnE)
SUC: ECTR2_76
SUT: SAT0131_00106
SUQ: HMPREF0772_10377
SUZ: MS7_0112
SUD: ST398NM01_0132
SUX: SAEMRSA15_00860
SUW: SATW20_01320(sbnE)
SUG: SAPIG0132
SUF: SARLGA251_00950(sbnE)
SAUA: SAAG_00604
SAUE: RSAU_000074
SAUN: SAKOR_00094
SAUS: SA40_0087(sbnE)
SAUU: SA957_0102(sbnE)
SAUG: SA268_0099(sbnE)
SAUZ: SAZ172_0131(sbnE)
SAUT: SAI1T1_2000760
SAUJ: SAI2T2_1000760
SAUK: SAI3T3_1000760
SAUQ: SAI4T8_1000760
SAUV: SAI7S6_1000760
SAUW: SAI5S5_1000760
SAUX: SAI6T6_1000760
SAUY: SAI8T7_1000760
SAUF: X998_0099
SAB: SAB0059
SUY: SA2981_0121(sbnE)
SAUB: C248_0108
SAUM: BN843_1220
SAUC: CA347_131
SAUR: SABB_01720(sbnE)
SAUI: AZ30_00625
SAUD: CH52_05120
SAMS: NI36_00520
SUH: SAMSHR1132_00960
SSD: SPSINT_0338
SDT: SPSE_2120
SDP: NCTC12225_02337(iucA)
SHU: SHYC_00375(sbnE)
SSCH: LH95_00375
SSCZ: RN70_00595
SAGQ: EP23_09750
SEQO: SE1039_25070
SLZ: B5P37_07610
SARL: SAP2_02070
SSH: NCTC13712_00073(iucA_1)
SRAI: LN051_00250
SFF: FOB90_07415
BAGR: BA6348_15335
BHUI: LOK74_15440
BBOR: RFB14_00525
PPOL: X809_04485
PTA: HPL003_19610
PPEO: ABE82_04640
PNP: IJ22_24510
POW: IJ21_46100
PXL: BS614_08680
PKB: B4V02_14045
PAIH: ASL14_00120
PPSC: EHS13_31660
PBAC: HUB98_21830
PKA: PQ456_10715
PMAH: PTQ21_17775
PMAE: LMZ02_21545
ANX: ACH33_02505
ATHE: K3F53_16445
SACA: FFV09_03670
TAB: CIG75_16795
LFB: C1X05_04250
KPUL: GXN76_14215
PABS: JIR001_05130(sbnE)
SACG: FDZ84_26450
PMAD: BAY61_28585
 » show all
Reference
1  [PMID:14688077]
  Authors
Dale SE, Doherty-Kirby A, Lajoie G, Heinrichs DE
  Title
Role of siderophore biosynthesis in virulence of Staphylococcus aureus: identification and characterization of genes involved in production of a siderophore.
  Journal
Infect Immun 72:29-37 (2004)
DOI:10.1128/IAI.72.1.29-37.2004
  Sequence
[sam:MW0093]
Reference
2  [PMID:19775248]
  Authors
Cheung J, Beasley FC, Liu S, Lajoie GA, Heinrichs DE
  Title
Molecular characterization of staphyloferrin B biosynthesis in Staphylococcus aureus.
  Journal
Mol Microbiol 74:594-608 (2009)
DOI:10.1111/j.1365-2958.2009.06880.x
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.54
IUBMB Enzyme Nomenclature: 6.3.2.54
ExPASy - ENZYME nomenclature database: 6.3.2.54
BRENDA, the Enzyme Database: 6.3.2.54
LinkDB

KEGG   REACTION: R12308
Entry
R12308                      Reaction                               
Name
L-2,3-diaminopropanoate:citrate ligase (AMP, 2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate-forming)
Definition
ATP + L-2,3-Diaminopropanoate + Citrate <=> AMP + Diphosphate + 2-[(L-Alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate
Equation
C00002 + C03401 + C00158 <=> C00020 + C00013 + C22072
Reaction class
RC00090  C00158_C22072
RC00096  C03401_C22072
Enzyme
6.3.2.54
Pathway
rn00997  Biosynthesis of various other secondary metabolites
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00875  Staphyloferrin B biosynthesis, L-serine => staphyloferrin B
Brite
Enzymatic reactions [BR:br08201]
 6. Ligase reactions
  6.3  Forming carbon-nitrogen bonds
   6.3.2  Acid-D-amino-acid ligases (peptide synthases)
    6.3.2.54
     R12308  ATP + L-2,3-Diaminopropanoate + Citrate <=> AMP + Diphosphate + 2-[(L-Alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate
Orthology
K23372  L-2,3-diaminopropanoate---citrate ligase [EC:6.3.2.54]
LinkDB

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