KEGG ORTHOLOGY: K24276

ORTHOLOGY: K24276

Entry

K24276                      KO

Symbol

VAMT

Name

vanillin aminotransferase [EC:2.6.1.119]

Pathway

map00996

Biosynthesis of various alkaloids

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Reaction

R12622

L-alanine:vanillin aminotransferase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00996 Biosynthesis of various alkaloids
    K24276  VAMT; vanillin aminotransferase
Enzymes [BR:ko01000]
2. Transferases
  2.6  Transferring nitrogenous groups
   2.6.1  Transaminases
    2.6.1.119  vanillin aminotransferase
     K24276  VAMT; vanillin aminotransferase

Genes

SLY:

SPEN:

SOT:

SSTN:

SDUL:

CANN:

LBB:

NAU:

Reference

PMID:22921003

Authors

Gururaj HB, Padma MN, Giridhar P, Ravishankar GA

Title

Functional validation of Capsicum frutescens aminotransferase gene involved in vanillylamine biosynthesis using Agrobacterium mediated genetic transformation studies in Nicotiana tabacum and Capsicum frutescens calli cultures.

Journal

Plant Sci 195:96-105 (2012)
DOI:10.1016/j.plantsci.2012.06.014

Sequence

[cann:107854420]

LinkDB

ENZYME: 2.6.1.119

Entry

EC 2.6.1.119                Enzyme

Name

vanillin aminotransferase;
VAMT (gene name)

Class

Transferases;
Transferring nitrogenous groups;
Transaminases

Sysname

L-alanine:vanillin aminotransferase

Reaction(IUBMB)

L-alanine + vanillin = pyruvate + vanillylamine [RN:R12622]

Reaction(KEGG)

R12622

Substrate

L-alanine [CPD:C00041];
vanillin [CPD:C00755]

Product

pyruvate [CPD:C00022];
vanillylamine [CPD:C16666]

Comment

The enzyme participates in the biosynthesis of capsaicinoids in pungent cultivars of Capsicum sp. In vivo it has only been assayed in the reverse direction, where the preferred amino group acceptors were found to be pyruvate and oxaloacetate.

History

EC 2.6.1.119 created 2020

Pathway

ec00996

Biosynthesis of various alkaloids

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K24276

vanillin aminotransferase

Genes

SLY:

101258391

SPEN:

107027278

SOT:

102597871

SSTN:

125878196

SDUL:

129890408

CANN:

107854420

LBB:

132636719

NAU:

109222880

Reference

Authors

Curry J, Aluru M, Mendoza M, Nevarez J, Melendrez M, O'Connell MA.

Title

Transcripts for possible capsaicinoid biosynthetic genes are differentially accumulated in pungent and non-pungent Capsicum spp.

Journal

Plant Sci 148:47-57 (1999)

Reference

2 [PMID:17999078]

Authors

del Rosario Abraham-Juarez M, del Carmen Rocha-Granados M, Lopez MG, Rivera-Bustamante RF, Ochoa-Alejo N

Title

Virus-induced silencing of Comt, pAmt and Kas genes results in a reduction of capsaicinoid accumulation in chili pepper fruits.

Journal

Planta 227:681-95 (2008)
DOI:10.1007/s00425-007-0651-7

Reference

3 [PMID:19473323]

Authors

Lang Y, Kisaka H, Sugiyama R, Nomura K, Morita A, Watanabe T, Tanaka Y, Yazawa S, Miwa T.

Title

Functional loss of pAMT results in biosynthesis of capsinoids, capsaicinoid analogs, in Capsicum annuum cv. CH-19 Sweet.

Journal

Plant J 59:953-61 (2009)
DOI:10.1111/j.1365-313X.2009.03921.x

Reference

4 [PMID:22921003]

Authors

Gururaj HB, Padma MN, Giridhar P, Ravishankar GA

Title

Journal

Plant Sci 195:96-105 (2012)
DOI:10.1016/j.plantsci.2012.06.014

Sequence

[cann:107854420]

Reference

5 [PMID:24712445]

Authors

Weber N, Ismail A, Gorwa-Grauslund M, Carlquist M

Title

Biocatalytic potential of vanillin aminotransferase from Capsicum chinense.

Journal

BMC Biotechnol 14:25 (2014)
DOI:10.1186/1472-6750-14-25

Sequence

[cann:107854420]

Other DBs

ExplorEnz - The Enzyme Database:

2.6.1.119

IUBMB Enzyme Nomenclature:

2.6.1.119

ExPASy - ENZYME nomenclature database:

2.6.1.119

BRENDA, the Enzyme Database:

2.6.1.119

LinkDB

REACTION: R12622

Entry

R12622                      Reaction

Name

L-alanine:vanillin aminotransferase

Definition

4-Hydroxy-3-methoxy-benzaldehyde + L-Alanine <=> Vanillylamine + Pyruvate

Equation

C00755 + C00041 <=> C16666 + C00022

Reaction class

RC00048

C00755_C16666

Enzyme

2.6.1.119

Pathway

rn00996

Biosynthesis of various alkaloids

rn01100

Metabolic pathways

rn01110

Biosynthesis of secondary metabolites

Brite

Enzymatic reactions [BR:br08201]
2. Transferase reactions
  2.6  Transferring nitrogenous groups
   2.6.1  Transaminases
    2.6.1.119
     R12622  4-Hydroxy-3-methoxy-benzaldehyde + L-Alanine <=> Vanillylamine + Pyruvate

Orthology

K24276

vanillin aminotransferase [EC:2.6.1.119]

LinkDB

DBGET integrated database retrieval system