KEGG ORTHOLOGY: K25009

ORTHOLOGY: K25009

Entry

K25009                      KO

Symbol

E1.5.1.20

Name

methylenetetrahydrofolate reductase [NAD(P)H] [EC:1.5.1.20]

Pathway

map00670

One carbon pool by folate

map01100

Metabolic pathways

map01120

Microbial metabolism in diverse environments

Reaction

R01224

5-methyltetrahydrofolate:NADP+ oxidoreductase

R07168

5-methyltetrahydrofolate:NAD+ oxidoreductase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09108 Metabolism of cofactors and vitamins
   00670 One carbon pool by folate
    K25009  E1.5.1.20; methylenetetrahydrofolate reductase [NAD(P)H]
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.5  Acting on the CH-NH group of donors
   1.5.1  With NAD+ or NADP+ as acceptor
    1.5.1.20  methylenetetrahydrofolate reductase [NAD(P)H]
     K25009  E1.5.1.20; methylenetetrahydrofolate reductase [NAD(P)H]

Other DBs

GO:

0004489

Genes

LMA:	LMJF_36_6390

LIF:	LINJ_36_6670

LDO:	LDBPK_366670

LMI:	LMXM_36_6390

LMAT:

92510831(LSCM1_00677)

LBZ:	LBRM_35_6720

LPAN:

LPMP_356560

LOI:	92356693(LSCM4_00683)

LEIS:

94176326(GH5_00690)

LENR:

94167975(CUR178_00684)

LEIN:

94184455(JIQ42_00694)

PHET:

94287054(JKF63_00928)

» show all

Reference

PMID:17032644

Authors

Vickers TJ, Orsomando G, de la Garza RD, Scott DA, Kang SO, Hanson AD, Beverley SM.

Title

Biochemical and genetic analysis of methylenetetrahydrofolate reductase in Leishmania metabolism and virulence.

Journal

J Biol Chem 281:38150-8 (2006)
DOI:10.1074/jbc.M608387200

Sequence

[lma:LMJF_36_6390]

LinkDB

ENZYME: 1.5.1.20

Entry

EC 1.5.1.20                 Enzyme

Name

methylenetetrahydrofolate reductase [NAD(P)H];
MTHFR (gene name)

Class

Oxidoreductases;
Acting on the CH-NH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

5-methyltetrahydrofolate:NAD(P)+ oxidoreductase

Reaction(IUBMB)

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H + H+ [RN:R01224 R07168]

Reaction(KEGG)

R01224 R07168

Substrate

5-methyltetrahydrofolate [CPD:C00440];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]

Product

5,10-methylenetetrahydrofolate [CPD:C00143];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

A flavoprotein (FAD). The enzyme catalyses the reversible conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, playing an important role in folate metabolism by regulating the distribution of one-carbon moieties between cellular methylation reactions and nucleic acid synthesis. This enzyme, characterized from Protozoan parasites of the genus Leishmania, is unique among similar characterized eukaryotic enzymes in that it lacks the C-terminal allosteric regulatory domain (allowing it to catalyse a reversible reaction) and uses NADH and NADPH with equal efficiency under physiological conditions. cf. EC 1.5.1.53, methylenetetrahydrofolate reductase (NADPH); EC 1.5.1.54, methylenetetrahydrofolate reductase (NADH); and EC 1.5.7.1, methylenetetrahydrofolate reductase (ferredoxin).

History

EC 1.5.1.20 created 1978 as EC 1.1.1.171, transferred 1984 to EC 1.5.1.20 (EC 1.7.99.5 incorporated 2005), modified 2005., modified 2021, modified 2023

Pathway

ec00670

One carbon pool by folate

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K25009

methylenetetrahydrofolate reductase [NAD(P)H]

Genes

LMA:	LMJF_36_6390

LIF:	LINJ_36_6670

LDO:	LDBPK_366670

LMI:	LMXM_36_6390

LMAT:

92510831(LSCM1_00677)

LBZ:	LBRM_35_6720

LPAN:

LPMP_356560

LOI:	92356693(LSCM4_00683)

LEIS:

94176326(GH5_00690)

LENR:

94167975(CUR178_00684)

LEIN:

94184455(JIQ42_00694)

PHET:

94287054(JKF63_00928)

» show all

Reference

1 [PMID:17032644]

Authors

Vickers TJ, Orsomando G, de la Garza RD, Scott DA, Kang SO, Hanson AD, Beverley SM.

Title

Biochemical and genetic analysis of methylenetetrahydrofolate reductase in Leishmania metabolism and virulence.

Journal

J Biol Chem 281:38150-8 (2006)
DOI:10.1074/jbc.M608387200

Sequence

[lma:LMJF_36_6390]

Other DBs

ExplorEnz - The Enzyme Database:

1.5.1.20

IUBMB Enzyme Nomenclature:

1.5.1.20

ExPASy - ENZYME nomenclature database:

1.5.1.20

BRENDA, the Enzyme Database:

1.5.1.20

CAS:	71822-25-8

LinkDB

REACTION: R01224

Entry

R01224                      Reaction

Name

5-methyltetrahydrofolate:NADP+ oxidoreductase

Definition

5-Methyltetrahydrofolate + NADP+ <=> 5,10-Methylenetetrahydrofolate + NADPH + H+

Equation

C00440 + C00006 <=> C00143 + C00005 + C00080

Reaction class

RC00001

C00005_C00006

RC00081

C00143_C00440

Enzyme

1.5.1.20 1.5.1.53

Pathway

rn00670

One carbon pool by folate

rn01100

Metabolic pathways

rn01120

Microbial metabolism in diverse environments

Brite

Enzymatic reactions [BR:br08201]
1. Oxidoreductase reactions
  1.5  Acting on the CH-NH group of donors
   1.5.1  With NAD+ or NADP+ as acceptor
    1.5.1.20
     R01224  5-Methyltetrahydrofolate + NADP+ <=> 5,10-Methylenetetrahydrofolate + NADPH + H+
    1.5.1.53
     R01224  5-Methyltetrahydrofolate + NADP+ <=> 5,10-Methylenetetrahydrofolate + NADPH + H+

Orthology

K25004

methylenetetrahydrofolate reductase (NADPH) [EC:1.5.1.53]

K25009

methylenetetrahydrofolate reductase [NAD(P)H] [EC:1.5.1.20]

Other DBs

RHEA:

19820

LinkDB

REACTION: R07168

Entry

R07168                      Reaction

Name

5-methyltetrahydrofolate:NAD+ oxidoreductase

Definition

5-Methyltetrahydrofolate + NAD+ <=> 5,10-Methylenetetrahydrofolate + NADH + H+

Equation

C00440 + C00003 <=> C00143 + C00004 + C00080

Reaction class

RC00001

C00003_C00004

RC00081

C00143_C00440

Enzyme

1.5.1.20 1.5.1.54

Pathway

rn00670

One carbon pool by folate

rn00720

Other carbon fixation pathways

rn01100

Metabolic pathways

rn01120

Microbial metabolism in diverse environments

rn01200

Carbon metabolism

Module

M00377

Reductive acetyl-CoA pathway (Wood-Ljungdahl pathway)

Brite

Enzymatic reactions [BR:br08201]
1. Oxidoreductase reactions
  1.5  Acting on the CH-NH group of donors
   1.5.1  With NAD+ or NADP+ as acceptor
    1.5.1.20
     R07168  5-Methyltetrahydrofolate + NAD+ <=> 5,10-Methylenetetrahydrofolate + NADH + H+
    1.5.1.54
     R07168  5-Methyltetrahydrofolate + NAD+ <=> 5,10-Methylenetetrahydrofolate + NADH + H+
Overall reaction [br08210.html]
Carbon fixation
  R07168

Orthology

K00297

methylenetetrahydrofolate reductase (NADH) [EC:1.5.1.54]

K24042

methionine synthase / methylenetetrahydrofolate reductase (NADH) [EC:2.1.1.13 1.5.1.54]

K25009

methylenetetrahydrofolate reductase [NAD(P)H] [EC:1.5.1.20]

Other DBs

RHEA:

19824

LinkDB

DBGET integrated database retrieval system