KEGG   ORTHOLOGY: K25305
Entry
K25305                      KO                                     
Symbol
dmfA1
Name
N,N-dimethylformamidase small subunit [EC:3.5.1.56]
Pathway
map00630  Glyoxylate and dicarboxylate metabolism
map01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00630 Glyoxylate and dicarboxylate metabolism
    K25305  dmfA1; N,N-dimethylformamidase small subunit
Enzymes [BR:ko01000]
 3. Hydrolases
  3.5  Acting on carbon-nitrogen bonds, other than peptide bonds
   3.5.1  In linear amides
    3.5.1.56  N,N-dimethylformamidase
     K25305  dmfA1; N,N-dimethylformamidase small subunit
Other DBs
RN: R02509
GO: 0050116
Genes
METD: C0214_26885
PAMN: JCM7685_0286(dmfA1)
SINE: KI385_41170
AG: BAA90663(dmfA1)
Reference
  Authors
Hasegawa Y, Tokuyama T, Iwaki H
  Title
Cloning and expression of the N,N-dimethylformamidase gene from Alcaligenes sp. strain KUFA-1.
  Journal
Biosci Biotechnol Biochem 63:2091-6 (1999)
DOI:10.1271/bbb.63.2091
  Sequence
LinkDB

KEGG   ORTHOLOGY: K03418
Entry
K03418                      KO                                     
Symbol
dmfA2
Name
N,N-dimethylformamidase large subunit [EC:3.5.1.56]
Pathway
map00630  Glyoxylate and dicarboxylate metabolism
map01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00630 Glyoxylate and dicarboxylate metabolism
    K03418  dmfA2; N,N-dimethylformamidase large subunit
Enzymes [BR:ko01000]
 3. Hydrolases
  3.5  Acting on carbon-nitrogen bonds, other than peptide bonds
   3.5.1  In linear amides
    3.5.1.56  N,N-dimethylformamidase
     K03418  dmfA2; N,N-dimethylformamidase large subunit
Other DBs
RN: R02509
GO: 0050116
Genes
AG: BAA90664(dmfA2)
Reference
  Authors
Hasegawa Y, Tokuyama T, Iwaki H
  Title
Cloning and expression of the N,N-dimethylformamidase gene from Alcaligenes sp. strain KUFA-1.
  Journal
Biosci Biotechnol Biochem 63:2091-6 (1999)
DOI:10.1271/bbb.63.2091
  Sequence
LinkDB

KEGG   ENZYME: 3.5.1.56
Entry
EC 3.5.1.56                 Enzyme                                 
Name
N,N-dimethylformamidase;
dimethylformamidase;
DMFase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
N,N-dimethylformamide amidohydrolase
Reaction(IUBMB)
N,N-dimethylformamide + H2O = dimethylamine + formate [RN:R02509]
Reaction(KEGG)
R02509
Substrate
N,N-dimethylformamide [CPD:C03134];
H2O [CPD:C00001]
Product
dimethylamine [CPD:C00543];
formate [CPD:C00058]
Comment
An iron protein. Also acts on N-ethylformamide and N-methyl-formamide and, more slowly, on N,N-diethylformamide, N,N-dimethylacetamide and unsubstituted acyl amides.
History
EC 3.5.1.56 created 1989
Pathway
ec00630  Glyoxylate and dicarboxylate metabolism
ec01100  Metabolic pathways
Orthology
K03418  N,N-dimethylformamidase large subunit
K25305  N,N-dimethylformamidase small subunit
Genes
METDC0214_26885
PAMNJCM7685_0286(dmfA1)
SINEKI385_41170
Reference
1  [PMID:3732281]
  Authors
Schar HP, Holzmann W, Ramos Tombo GM, Ghisalba O.
  Title
Purification and characterization of N,N-dimethylformamidase from Pseudomonas DMF 3/3.
  Journal
Eur J Biochem 158:469-75 (1986)
DOI:10.1111/j.1432-1033.1986.tb09778.x
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.56
IUBMB Enzyme Nomenclature: 3.5.1.56
ExPASy - ENZYME nomenclature database: 3.5.1.56
BRENDA, the Enzyme Database: 3.5.1.56
CAS: 104645-73-0
LinkDB

KEGG   REACTION: R02509
Entry
R02509                      Reaction                               
Name
N,N-Dimethylformamide amidohydrolase
Definition
N,N-Dimethylformamide + H2O <=> Dimethylamine + Formate
Equation
Reaction class
RC00111  C00058_C03134
RC00731  C00543_C03134
Enzyme
Pathway
rn00630  Glyoxylate and dicarboxylate metabolism
rn01100  Metabolic pathways
Orthology
K03418  N,N-dimethylformamidase large subunit [EC:3.5.1.56]
K25305  N,N-dimethylformamidase small subunit [EC:3.5.1.56]
Other DBs
RHEA: 19520
LinkDB

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