KEGG ORTHOLOGY: K26930

ORTHOLOGY: K26930

Entry

K26930                      KO

Symbol

mtqA

Name

[methyl-Co(III) quaternary-amine-specific corrinoid protein]---tetrahydrofolate methyltransferase [EC:2.1.1.389]

Pathway

map00670

One carbon pool by folate

map00720

Other carbon fixation pathways

Reaction

R02289

5-methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09102 Energy metabolism
   00720 Other carbon fixation pathways
    K26930  mtqA; [methyl-Co(III) quaternary-amine-specific corrinoid protein]---tetrahydrofolate methyltransferase
  09108 Metabolism of cofactors and vitamins
   00670 One carbon pool by folate
    K26930  mtqA; [methyl-Co(III) quaternary-amine-specific corrinoid protein]---tetrahydrofolate methyltransferase
Enzymes [BR:ko01000]
2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.389  [methyl-Co(III) quaternary-amine-specific corrinoid protein]---tetrahydrofolate methyltransferase
     K26930  mtqA; [methyl-Co(III) quaternary-amine-specific corrinoid protein]---tetrahydrofolate methyltransferase

Other DBs

COG:

COG1410

Genes

DVR:	Dvar_12500 Dvar_13710 Dvar_66960 Dvar_68860

ELM:

ELI_0371

EMT:	CPZ25_011950

ELIM:

B2M23_04840

Reference

PMID:31341018

Authors

Picking JW, Behrman EJ, Zhang L, Krzycki JA.

Title

MtpB, a member of the MttB superfamily from the human intestinal acetogen Eubacterium limosum, catalyzes proline betaine demethylation.

Journal

J Biol Chem 294:13697-13707 (2019)
DOI:10.1074/jbc.RA119.009886

Sequence

[elim:B2M23_04840]

LinkDB

ENZYME: 2.1.1.389

Entry

EC 2.1.1.389                Enzyme

Name

[methyl-Co(III) quaternary-amine-specific corrinoid protein]---tetrahydrofolate methyltransferase;
mtqA (gene name) (ambiguous);
[methyl-Co(III) MtqC corrinoid protein]---tetrahydrofolate methyltransferase

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

[methyl-Co(III) quaternary-amine-specific corrinoid protein]:tetrahydrofolate methyltransferase

Reaction(IUBMB)

a [methyl-Co(III) quaternary-amine-specific corrinoid protein] + tetrahydrofolate = N5-methyltetrahydrofolate + a [Co(I) quaternary-amine-specific corrinoid protein] [RN:R02289]

Reaction(KEGG)

R02289

Substrate

[methyl-Co(III) quaternary-amine-specific corrinoid protein];
tetrahydrofolate [CPD:C00101]

Product

N5-methyltetrahydrofolate;
[Co(I) quaternary-amine-specific corrinoid protein]

Comment

The enzyme, characterized from the acetogenic gut bacterium Eubacterium limosum, participates in a pathway for the degradation of some quaternary amine compounds (L-proline betaine and L-carnitine). The enzyme catalyses the transfer of a methyl group bound to the cobalt cofactor of a dedicated corrinoid protein (bacterial MtqC) to tetrahydrofolate. The resulting 5-methyltetrahydrofolate is processed to acetyl-CoA via the Wood---Ljungdahl pathway.

History

EC 2.1.1.389 created 2023

Pathway

ec00670

One carbon pool by folate

ec00720

Other carbon fixation pathways

Orthology

K26930

[methyl-Co(III) quaternary-amine-specific corrinoid protein]---tetrahydrofolate methyltransferase

Genes

DVR:	Dvar_12500 Dvar_13710 Dvar_66960 Dvar_68860

ELM:

ELI_0371

EMT:	CPZ25_011950

ELIM:

B2M23_04840

AMAJ:

ACIUZZ_00235 ACIUZZ_01935 ACIUZZ_01955 ACIUZZ_03970 ACIUZZ_06335 ACIUZZ_06580 ACIUZZ_06990 ACIUZZ_07005 ACIUZZ_07045 ACIUZZ_07255 ACIUZZ_08070 ACIUZZ_12420 ACIUZZ_13535 ACIUZZ_14195 ACIUZZ_15940 ACIUZZ_15965 ACIUZZ_16845 ACIUZZ_17475 ACIUZZ_17675 ACIUZZ_18275

Reference

1 [PMID:31341018]

Authors

Picking JW, Behrman EJ, Zhang L, Krzycki JA.

Title

MtpB, a member of the MttB superfamily from the human intestinal acetogen Eubacterium limosum, catalyzes proline betaine demethylation.

Journal

J Biol Chem 294:13697-13707 (2019)
DOI:10.1074/jbc.RA119.009886

Sequence

[elim:B2M23_04840]

Reference

2 [PMID:32571881]

Authors

Kountz DJ, Behrman EJ, Zhang L, Krzycki JA.

Title

MtcB, a member of the MttB superfamily from the human gut acetogen Eubacterium limosum, is a cobalamin-dependent carnitine demethylase.

Journal

J Biol Chem 295:11971-11981 (2020)
DOI:10.1074/jbc.RA120.012934

Sequence

[elim:B2M23_04840]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.389

IUBMB Enzyme Nomenclature:

2.1.1.389

ExPASy - ENZYME nomenclature database:

2.1.1.389

BRENDA, the Enzyme Database:

2.1.1.389

LinkDB

REACTION: R02289

Entry

R02289                      Reaction

Name

5-methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase;
[methyl-Co(III) glycine betaine-specific corrinoid protein]:tetrahydrofolate N-methyltransferase

Definition

5-Methyltetrahydrofolate + Co(I) corrinoid protein + H+ <=> Methyl-Co(III) corrinoid protein + Tetrahydrofolate

Equation

C00440 + C06021 + C00080 <=> C06020 + C00101

Reaction class

RC00113

C00101_C00440

RC01144

C06020_C06021

RC02871

C00440_C06020

Enzyme

2.1.1.258 2.1.1.378 2.1.1.385 2.1.1.389

Pathway

rn00670

One carbon pool by folate

rn00720

Other carbon fixation pathways

rn01100

Metabolic pathways

Module

M00377

Reductive acetyl-CoA pathway (Wood-Ljungdahl pathway)

Brite

Enzymatic reactions [BR:br08201]
2. Transferase reactions
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.258
     R02289  5-Methyltetrahydrofolate + Co(I) corrinoid protein + H+ <=> Methyl-Co(III) corrinoid protein + Tetrahydrofolate
    2.1.1.378
     R02289  5-Methyltetrahydrofolate + Co(I) corrinoid protein + H+ <=> Methyl-Co(III) corrinoid protein + Tetrahydrofolate
    2.1.1.385
     R02289  5-Methyltetrahydrofolate + Co(I) corrinoid protein + H+ <=> Methyl-Co(III) corrinoid protein + Tetrahydrofolate
    2.1.1.389
     R02289  5-Methyltetrahydrofolate + Co(I) corrinoid protein + H+ <=> Methyl-Co(III) corrinoid protein + Tetrahydrofolate

Orthology

K15023

5-methyltetrahydrofolate corrinoid/iron sulfur protein methyltransferase [EC:2.1.1.258]

K25221

[methyl-Co(III) glycine betaine-specific corrinoid protein]---tetrahydrofolate methyltransferase [EC:2.1.1.378]

K25915

[methyl-Co(III) methoxylated-aromatic-compound-specific corrinoid protein]---tetrahydrofolate methyltransferase [EC:2.1.1.385]

K26930

[methyl-Co(III) quaternary-amine-specific corrinoid protein]---tetrahydrofolate methyltransferase [EC:2.1.1.389]

Other DBs

RHEA:

45203 66003 75874 75878

LinkDB

DBGET integrated database retrieval system