Entry Name meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase II;
Class Transferases;BRITE hierarchy
Sysname ultra-long-chain di-unsaturated fattyl acyl-[acyl-carrier protein]:malonyl-[acyl-carrier protein] C-acyltransferase (decarboxylating)
Reaction(IUBMB) an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + a holo-[acyl-carrier protein] [RN:
R13270 ]
Reaction(KEGG) Substrate ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] [CPD:
C22869 ];
malonyl-[acyl-carrier protein] [CPD:
C01209 ]
Product ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] [CPD:
C22870 ];
CO2 [CPD:
C00011 ];
holo-[acyl-carrier protein] [CPD:
C00229 ]
Comment The enzyme is part of the fatty acid synthase (FAS) II system of mycobacteria, which extends modified products of the FAS I system, eventually forming meromycolic acids that are incorporated into mycolic acids. Meromycolic acids consist of a long chain, typically 50-60 carbons, which is functionalized by different groups.Two 3-oxoacyl-(acyl carrier protein) synthases function within the FAS II system, encoded by the kasA and kasB genes. The two enzymes share some sequence identity but function independently on separate sets of substrates. KasB differs from KasA (EC
2.3.1.293 , meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase I), by preferring longer substrates (closer to the upper limit), which also contain two double bonds.
History EC 2.3.1.294 created 2019
Pathway ec01110 Biosynthesis of secondary metabolites
Orthology K27070 meromycolic acid 3-oxoacyl-[acyl-carrier protein] synthase II
Genes MPA : MAP_1999(kasB) MAP_3485(kasB)
MCHI : AN480_11270 AN480_22070
MMAL : CKJ54_09720 CKJ54_20115
MMAN : MMAN_18030(kasB_1) MMAN_30190(kasB_2)
MSER : MTY59_05580(kasB) MTY59_37850(kasB)
MSA : Mycsm_00734 Mycsm_03981
MMAE : MMARE11_32400(kasB)
MYV : G155_18580 G155_24920
MYE : AB431_04975 AB431_19265
MDX : BTO20_06565 BTO20_13085
MSHG : MSG_01053(kasB_1) MSG_03171(kasB_2)
MFJ : MFLOJ_00450(kasB_1) MFLOJ_23320(kasB_2)
MSTO : MSTO_01110(kasB_1) MSTO_27280(kasB_2)
MSIM : MSIM_14500(kasB_1) MSIM_45150(kasB_2)
MSAK : MSAS_12210(kasB_1) MSAS_12510(kasB_2)
MKU : I2456_16060 I2456_16415
MLW : MJO58_05580(kasB) MJO58_16835(kasB)
MXE : MYXE_20720(kasB) MYXE_43070
MNV : MNVI_22730 MNVI_45160(kasB)
MPAG : C0J29_07930 C0J29_18645
MNM : MNVM_02380(kasB) MNVM_40280
MGOR : H0P51_17365 H0P51_25650
MCOO : MCOO_12830(kasB_1) MCOO_39080(kasB_2)
MBAI : MB901379_03011(kasB)
MSEO : MSEO_14160(kasB_1) MSEO_19580(kasB_2)
MHEK : JMUB5695_02081(kasB) JMUB5695_04192
MGAU : MGALJ_21320 MGALJ_52620
MBRD : MBRA_10910 MBRA_35610(kasB)
MPAA : MKK62_10055(kasB) MKK62_21435
MMEH : M5I08_06355 M5I08_12040(kasB)
MKY : IWGMT90018_24120 IWGMT90018_57110
MWU : PT015_07480(kasB) PT015_20985
MSM : MSMEG_1204 MSMEG_4328
MSG : MSMEI_1170(kasB) MSMEI_4228(kasB)
MSB : LJ00_05995 LJ00_21450
MSN : LI99_05995 LI99_21455
MSH : LI98_05995 LI98_21460
MFT : XA26_37940 XA26_50520
MPHL : MPHLCCUG_01036(kasB_1) MPHLCCUG_03393(kasB_2)
MLL : B1R94_04715 B1R94_10585
MRH : MycrhN_0961 MycrhN_4434
MTHN : 4412656_01785(kasB)
MHAS : MHAS_00020(kasB_1) MHAS_01448(kasB_2)
MDR : MDOR_04350 MDOR_24820
MAUU : NCTC10437_03514(kasB_1)
MMAG : MMAD_09900(kasB_1) MMAD_35140 MMAD_50950
MMOR : MMOR_29430 MMOR_56170
MAIC : MAIC_34220 MAIC_46930
MGAD : MGAD_02940 MGAD_51510
MANY : MANY_19080 MANY_48670
MMAT : MMAGJ_40560 MMAGJ_73570
MFG : K6L26_13125 K6L26_26205
MFLV : NCTC10271_01898(kasB_2) NCTC10271_04318(kasB_3)
MCEE : MCEL_03180 MCEL_25410
MMON : EWR22_04110 EWR22_17425
MGO : AFA91_18465 AFA91_32665
MHOL : K3U96_10645 K3U96_22490
MSEN : K3U95_05495 K3U95_16000
MPAE : K0O64_04540 K0O64_18630
MCRO : MI149_05220 MI149_19455(kasB)
MAUS : JN090_19085 JN090_24550
MJD : JDM601_1649(kasB) JDM601_2027(kasB)
MTER : 4434518_01562(kasB_1) 4434518_01976(kasB_2)
MHIB : MHIB_10830(kasB) MHIB_26020
MHER : K3U94_09075 K3U94_17975
MVM : MJO54_09165(kasB) MJO54_18130(kasB)
» show all Taxonomy Reference Authors Schaeffer ML, Agnihotri G, Volker C, Kallender H, Brennan PJ, Lonsdale JT
Title Purification and biochemical characterization of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthases KasA and KasB.
Journal Sequence Reference Authors Gao LY, Laval F, Lawson EH, Groger RK, Woodruff A, Morisaki JH, Cox JS, Daffe M, Brown EJ
Title Requirement for kasB in Mycobacterium mycolic acid biosynthesis, cell wall impermeability and intracellular survival: implications for therapy.
Journal Sequence Reference Authors Molle V, Brown AK, Besra GS, Cozzone AJ, Kremer L
Title The condensing activities of the Mycobacterium tuberculosis type II fatty acid synthase are differentially regulated by phosphorylation.
Journal Sequence Reference Authors Bhatt A, Fujiwara N, Bhatt K, Gurcha SS, Kremer L, Chen B, Chan J, Porcelli SA, Kobayashi K, Besra GS, Jacobs WR Jr
Title Deletion of kasB in Mycobacterium tuberculosis causes loss of acid-fastness and subclinical latent tuberculosis in immunocompetent mice.
Journal Reference Authors Yamada H, Bhatt A, Danev R, Fujiwara N, Maeda S, Mitarai S, Chikamatsu K, Aono A, Nitta K, Jacobs WR Jr, Nagayama K
Title Non-acid-fastness in Mycobacterium tuberculosis DeltakasB mutant correlates with the cell envelope electron density.
Journal Reference Authors Vilcheze C, Molle V, Carrere-Kremer S, Leiba J, Mourey L, Shenai S, Baronian G, Tufariello J, Hartman T, Veyron-Churlet R, Trivelli X, Tiwari S, Weinrick B, Alland D, Guerardel Y, Jacobs WR Jr, Kremer L
Title Phosphorylation of KasB regulates virulence and acid-fastness in Mycobacterium tuberculosis.
Journal Sequence Other DBs ExPASy - ENZYME nomenclature database: 2.3.1.294
LinkDB All DBs
