KEGG   ORTHOLOGY: K27839
Entry
K27839                      KO                                     
Symbol
AKS1
Name
akuammiline synthase 1 [EC:2.3.1.322]
Pathway
map00901  Indole alkaloid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R13362  acetyl-CoA:rhazimol O-acetyltransferase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00901 Indole alkaloid biosynthesis
    K27839  AKS1; akuammiline synthase 1
Enzymes [BR:ko01000]
 2. Transferases
  2.3  Acyltransferases
   2.3.1  Transferring groups other than aminoacyl groups
    2.3.1.322  akuammiline synthase
     K27839  AKS1; akuammiline synthase 1
Genes
CARB: 113688981 113690673 113722677 113722702 113723606 113743328
AGRV: 141682904 141685014 141710755
AG: URS65387(AKS1)
Reference
PMID:36349266
  Authors
Wang Z, Xiao Y, Wu S, Chen J, Li A, Tatsis EC.
  Title
Deciphering and reprogramming the cyclization regioselectivity in bifurcation of indole alkaloid biosynthesis.
  Journal
Chem Sci 13:12389-12395 (2022)
DOI:10.1039/d2sc03612f
  Sequence
[ag:URS65387]
LinkDB

KEGG   ORTHOLOGY: K27840
Entry
K27840                      KO                                     
Symbol
AKS2
Name
akuammiline synthase 2 [EC:2.3.1.322]
Pathway
map00901  Indole alkaloid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R13362  acetyl-CoA:rhazimol O-acetyltransferase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00901 Indole alkaloid biosynthesis
    K27840  AKS2; akuammiline synthase 2
Enzymes [BR:ko01000]
 2. Transferases
  2.3  Acyltransferases
   2.3.1  Transferring groups other than aminoacyl groups
    2.3.1.322  akuammiline synthase
     K27840  AKS2; akuammiline synthase 2
Genes
CARB: 113730337 140036548
AG: URS65388(AKS2)
Reference
PMID:36349266
  Authors
Wang Z, Xiao Y, Wu S, Chen J, Li A, Tatsis EC.
  Title
Deciphering and reprogramming the cyclization regioselectivity in bifurcation of indole alkaloid biosynthesis.
  Journal
Chem Sci 13:12389-12395 (2022)
DOI:10.1039/d2sc03612f
  Sequence
[ag:URS65388]
LinkDB

KEGG   ENZYME: 2.3.1.322
Entry
EC 2.3.1.322                Enzyme                                 
Name
akuammiline synthase;
AsAKS1;
AsAKS2
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
acetyl-CoA:rhazimol O-acetyltransferase
Reaction(IUBMB)
acetyl-CoA + rhazimol = CoA + akuammiline [RN:R13362]
Reaction(KEGG)
R13362
Substrate
acetyl-CoA [CPD:C00024];
rhazimol [CPD:C22950]
Product
CoA [CPD:C00010];
akuammiline [CPD:C22951]
Comment
Isolated from the plant Alstonia scholaris (blackboard tree).
History
EC 2.3.1.322 created 2024
Pathway
ec00901  Indole alkaloid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K27839  akuammiline synthase 1
K27840  akuammiline synthase 2
Genes
CARB: 113688981 113690673 113722677 113722702 113723606 113730337 113743328 140036548
AGRV: 141682904 141685014 141710755
Reference
1  [PMID:36349266]
  Authors
Wang Z, Xiao Y, Wu S, Chen J, Li A, Tatsis EC.
  Title
Deciphering and reprogramming the cyclization regioselectivity in bifurcation of indole alkaloid biosynthesis.
  Journal
Chem Sci 13:12389-12395 (2022)
DOI:10.1039/d2sc03612f
  Sequence
[ag:URS65387 URS65388]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.322
IUBMB Enzyme Nomenclature: 2.3.1.322
ExPASy - ENZYME nomenclature database: 2.3.1.322
BRENDA, the Enzyme Database: 2.3.1.322
LinkDB

KEGG   REACTION: R13362
Entry
R13362                      Reaction                               
Name
acetyl-CoA:rhazimol O-acetyltransferase
Definition
Acetyl-CoA + Rhazimol <=> CoA + Akuammiline
Equation
C00024 + C22950 <=> C00010 + C22951
Enzyme
2.3.1.322
Pathway
rn00901  Indole alkaloid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 2. Transferase reactions
  2.3  Acyltransferases
   2.3.1  Transferring groups other than aminoacyl groups
    2.3.1.322
     R13362  Acetyl-CoA + Rhazimol <=> CoA + Akuammiline
Orthology
K27839  akuammiline synthase 1 [EC:2.3.1.322]
K27840  akuammiline synthase 2 [EC:2.3.1.322]
LinkDB

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