KEGG   PATHWAY: cpw00510Help
Entry
cpw00510                    Pathway                                

Name
N-Glycan biosynthesis - Coccidioides posadasii
Description
N-glycans or asparagine-linked glycans are major constituents of glycoproteins in eukaryotes. N-glycans are covalently attached to asparagine with the consensus sequence of Asn-X-Ser/Thr by an N-glycosidic bond, GlcNAc b1- Asn. Biosynthesis of N-glycans begins on the cytoplasmic face of the ER membrane with the transferase reaction of UDP-GlcNAc and the lipid-like precursor P-Dol (dolichol phosphate) to generate GlcNAc a1- PP-Dol. After sequential addition of monosaccharides by ALG glycosyltransferases [MD:M00055], the N-glycan precursor is attached by the OST (oligosaccharyltransferase) complex to the polypeptide chain that is being synthesized and translocated through the ER membrane. The protein-bound N-glycan precursor is subsequently trimmed, extended, and modified in the ER and Golgi by a complex series of reactions catalyzed by membrane-bound glycosidases and glycosyltransferases. N-glycans thus synthesized are classified into three types: high-mannose type, complex type, and hybrid type. Defects in N-glycan biosynthesis lead to a variety of human diseases known as congenital disorders of glycosylation [DS:H00118 H00119].
Class
Metabolism; Glycan biosynthesis and metabolism
BRITE hierarchy
Pathway map
cpw00510  N-Glycan biosynthesis
cpw00510

Ortholog table
Module
cpw_M00055  N-glycan precursor biosynthesis [PATH:cpw00510]
cpw_M00072  N-glycosylation by oligosaccharyltransferase [PATH:cpw00510]
cpw_M00073  N-glycan precursor trimming [PATH:cpw00510]
Other DBs
BSID: 173688
GO: 0006487
Organism
Coccidioides posadasii [GN:cpw]
Gene
CPC735_022230  UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative [KO:K01001] [EC:2.7.8.15]
CPC735_025570  glycosyl transferase, group 2 family protein [KO:K00729] [EC:2.4.1.117]
CPC735_056840  UDP-N-acetylglucosamine transferase subunit Alg13, putative [KO:K07432] [EC:2.4.1.141]
CPC735_003500  hypothetical protein [KO:K07441] [EC:2.4.1.141]
CPC735_006690  dolichol-phosphate mannosyltransferase, putative [KO:K00721] [EC:2.4.1.83]
CPC735_062150  hypothetical protein [KO:K09659]
CPC735_035820  hypothetical protein [KO:K03842] [EC:2.4.1.142]
CPC735_023190  glycosyl transferase, group 1 family protein [KO:K03843] [EC:2.4.1.257 2.4.1.132]
CPC735_003580  glycosyl transferase, group 1 family protein [KO:K03844] [EC:2.4.1.131]
CPC735_016250  ALG3 family protein [KO:K03845] [EC:2.4.1.258]
CPC735_008290  Alg9-like mannosyltransferase family protein [KO:K03846] [EC:2.4.1.261 2.4.1.259]
CPC735_056390  glycosyltransferase, putative [KO:K03847] [EC:2.4.1.260]
CPC735_039780  dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase, putative [KO:K03848] [EC:2.4.1.267]
CPC735_018140  dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase, putative [KO:K03849] [EC:2.4.1.265]
CPC735_020280  DIE2/ALG10 family protein [KO:K03850] [EC:2.4.1.256]
CPC735_038750  oligosaccharyl transferase, putative [KO:K07151] [EC:2.4.99.18]
CPC735_053430  Ribophorin I family protein [KO:K12666]
CPC735_022370  hypothetical protein [KO:K12667]
CPC735_071840  DAD family protein [KO:K12668]
CPC735_000560  OST3 / OST6 family protein [KO:K12669]
CPC735_031620  Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kD subunit containing protein [KO:K12670]
CPC735_012850  PAP2 superfamily protein [KO:K07252] [EC:3.6.1.43]
CPC735_012890  glucosidase I, putative [KO:K01228] [EC:3.2.1.106]
CPC735_030320  glucosidase II alpha subunit, putative [KO:K05546] [EC:3.2.1.207]
CPC735_001190  endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, putative [KO:K01230] [EC:3.2.1.113]
CPC735_008870  mannosyl-oligosaccharide alpha-1,2-mannosidase precursor, putative [KO:K01230] [EC:3.2.1.113]
CPC735_047310  Glycosyl hydrolase family 47 protein [KO:K01230] [EC:3.2.1.113]
CPC735_017170  endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, putative [KO:K01230] [EC:3.2.1.113]
CPC735_018310  class I alpha-mannosidase, putative [KO:K01230] [EC:3.2.1.113]
Compound
C00096  GDP-mannose
C00110  Dolichyl phosphate
C00381  Dolichol
C00621  Dolichyl diphosphate
C01246  Dolichyl beta-D-glucosyl phosphate
C03021  Protein asparagine
C03862  Dolichyl phosphate D-mannose
G00001  N-Acetyl-D-glucosaminyldiphosphodolichol
G00002  N,N'-Chitobiosyldiphosphodolichol
G00003   
G00004   
G00005   
G00006   
G00007   
G00008   
G00009   
G00010   
G00011   
G00012   
G00013   
G00014   
G00015   
G00016   
G00017   
G00018  DS 3
G00019   
G00020   
G00021   
G00022   
G00171   
G10526   
G10595   
G10596   
G10597   
G10598   
G10599   
Reference
PMID:9111134
  Authors
Schutzbach JS.
  Title
The role of the lipid matrix in the biosynthesis of dolichyl-linked oligosaccharides.
  Journal
Glycoconj J 14:175-82 (1997)
DOI:10.1023/A:1018533620002
Reference
  Authors
Oriol R, Martinez-Duncker I, Chantret I, Mollicone R, Codogno P.
  Title
Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate.
  Journal
Mol Biol Evol 19:1451-63 (2002)
DOI:10.1093/oxfordjournals.molbev.a004208
Reference
  Authors
Thiel C, Schwarz M, Peng J, Grzmil M, Hasilik M, Braulke T, Kohlschutter A, von Figura K, Lehle L, Korner C.
  Title
A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis.
  Journal
J Biol Chem 278:22498-505 (2003)
DOI:10.1074/jbc.M302850200
Reference
  Authors
Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N.
  Title
The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum.
  Journal
J Biol Chem 276:21828-40 (2001)
DOI:10.1074/jbc.M010896200
Reference
  Authors
Frank CG, Aebi M.
  Title
ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.
  Journal
Glycobiology 15:1156-63 (2005)
DOI:10.1093/glycob/cwj002
Reference
  Authors
O'Reilly MK, Zhang G, Imperiali B.
  Title
In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.
  Journal
Biochemistry 45:9593-603 (2006)
DOI:10.1021/bi060878o
KO pathway
ko00510   
LinkDB All DBs

DBGET integrated database retrieval system