KEGG   PATHWAY: elo00542
Entry
elo00542                    Pathway                                

Name
O-Antigen repeat unit biosynthesis - Escherichia coli O44:H18 042 (EAEC)
Description
O-antigen is a repetitive glycan polymer synthesized as the most variable part in the lipopolysaccharide (LPS) biosynthesis (map00541). Structural diversity of O-antigen repeat units results from genetic diversity as summarized here for completely sequenced Escherichia coli genomes, many of which are for pathogenic strains.
Class
Metabolism; Glycan biosynthesis and metabolism
Pathway map
elo00542  O-Antigen repeat unit biosynthesis
elo00542

Organism
Escherichia coli O44:H18 042 (EAEC) [GN:elo]
Gene
EC042_4161  rfe; undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase [KO:K02851] [EC:2.7.8.33 2.7.8.35]
EC042_2277  wbaD; putative glycosyltransferase [KO:K23202]
EC042_2278  wbaC; putative glycosyltransferase [KO:K23201]
Reference
  Authors
Riley JG, Menggad M, Montoya-Peleaz PJ, Szarek WA, Marolda CL, Valvano MA, Schutzbach JS, Brockhausen I
  Title
The wbbD gene of E. coli strain VW187 (O7:K1) encodes a UDP-Gal: GlcNAc{alpha}-pyrophosphate-R {beta}1,3-galactosyltransferase involved in the biosynthesis of O7-specific lipopolysaccharide.
  Journal
Glycobiology 15:605-13 (2005)
DOI:10.1093/glycob/cwi038
Reference
  Authors
Marolda CL, Feldman MF, Valvano MA
  Title
Genetic organization of the O7-specific lipopolysaccharide biosynthesis cluster of Escherichia coli VW187 (O7:K1).
  Journal
Microbiology 145 ( Pt 9):2485-95 (1999)
DOI:10.1099/00221287-145-9-2485
Reference
  Authors
Greenfield LK, Richards MR, Li J, Wakarchuk WW, Lowary TL, Whitfield C
  Title
Biosynthesis of the polymannose lipopolysaccharide O-antigens from Escherichia coli serotypes O8 and O9a requires a unique combination of single- and multiple-active site mannosyltransferases.
  Journal
J Biol Chem 287:35078-91 (2012)
DOI:10.1074/jbc.M112.401000
Reference
  Authors
Wang W, Perepelov AV, Feng L, Shevelev SD, Wang Q, Senchenkova SN, Han W, Li Y, Shashkov AS, Knirel YA, Reeves PR, Wang L
  Title
A group of Escherichia coli and Salmonella enterica O antigens sharing a common backbone structure.
  Journal
Microbiology 153:2159-67 (2007)
DOI:10.1099/mic.0.2007/004192-0
Reference
PMID:7517391
  Authors
Stevenson G, Neal B, Liu D, Hobbs M, Packer NH, Batley M, Redmond JW, Lindquist L, Reeves P
  Title
Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster.
  Journal
J Bacteriol 176:4144-56 (1994)
DOI:10.1128/jb.176.13.4144-4156.1994
Reference
PMID:9004408
  Authors
Reeves PR, Hobbs M, Valvano MA, Skurnik M, Whitfield C, Coplin D, Kido N, Klena J, Maskell D, Raetz CR, Rick PD.
  Title
Bacterial polysaccharide synthesis and gene nomenclature.
  Journal
Trends Microbiol 4:495-503 (1996)
DOI:10.1016/S0966-842X(97)82912-5
Reference
PMID:1280216
  Authors
Jann B, Shashkov AS, Gupta DS, Jann K.
  Title
The O18 antigens (lipopolysaccharides) of Escherichia coli. Structural characterization of the O18A, O18A1, O18B and O18B1-specific polysaccharides.
  Journal
Eur J Biochem 210:241-8 (1992)
DOI:10.1111/j.1432-1033.1992.tb17414.x
Reference
PMID:2482127
  Authors
Jansson PE, Kenne L, Widmalm G.
  Title
Structure of the O-antigen polysaccharide from Escherichia coli O18ac: a revision using computer-assisted structural analysis with the program CASPER.
  Journal
Carbohydr Res 193:322-5 (1989)
DOI:10.1016/0008-6215(89)85135-3
Reference
  Authors
Li D, Liu B, Chen M, Guo D, Guo X, Liu F, Feng L, Wang L
  Title
A multiplex PCR method to detect 14 Escherichia coli serogroups associated with urinary tract infections.
  Journal
J Microbiol Methods 82:71-7 (2010)
DOI:10.1016/j.mimet.2010.04.008
Reference
PMID:8839182
  Authors
Manca MC, Weintraub A, Widmalm G.
  Title
Structural studies of the Escherichia coli O26 O-antigen polysaccharide.
  Journal
Carbohydr Res 281:155-60 (1996)
DOI:10.1016/0008-6215(95)00333-9
Reference
  Authors
D'Souza JM, Wang L, Reeves P
  Title
Sequence of the Escherichia coli O26 O antigen gene cluster and identification of O26 specific genes.
  Journal
Gene 297:123-7 (2002)
DOI:10.1016/S0378-1119(02)00876-4
Reference
  Authors
Wang L, Huskic S, Cisterne A, Rothemund D, Reeves PR
  Title
The O-antigen gene cluster of Escherichia coli O55:H7 and identification of a new UDP-GlcNAc C4 epimerase gene.
  Journal
J Bacteriol 184:2620-5 (2002)
DOI:10.1128/JB.184.10.2620-2625.2002
Reference
  Authors
Liu XW, Xia C, Li L, Guan WY, Pettit N, Zhang HC, Chen M, Wang PG
  Title
Characterization and synthetic application of a novel beta1,3-galactosyltransferase from Escherichia coli O55:H7.
  Journal
Bioorg Med Chem 17:4910-5 (2009)
DOI:10.1016/j.bmc.2009.06.005
Reference
  Authors
Fitzgerald C, Collins M, van Duyne S, Mikoleit M, Brown T, Fields P
  Title
Multiplex, bead-based suspension array for molecular determination of common Salmonella serogroups.
  Journal
J Clin Microbiol 45:3323-34 (2007)
DOI:10.1128/JCM.00025-07
Reference
  Authors
Liu B, Knirel YA, Feng L, Perepelov AV, Senchenkova SN, Reeves PR, Wang L
  Title
Structural diversity in Salmonella O antigens and its genetic basis.
  Journal
FEMS Microbiol Rev 38:56-89 (2014)
DOI:10.1111/1574-6976.12034
Reference
PMID:1291048
  Authors
Gamian A, Romanowska E, Ulrich J, Defaye J.
  Title
The structure of the sialic acid-containing Escherichia coli O104 O-specific polysaccharide and its linkage to the core region in lipopolysaccharide.
  Journal
Carbohydr Res 236:195-208 (1992)
DOI:10.1016/0008-6215(92)85016-S
Reference
  Authors
Wang L, Briggs CE, Rothemund D, Fratamico P, Luchansky JB, Reeves PR
  Title
Sequence of the E. coli O104 antigen gene cluster and identification of O104 specific genes.
  Journal
Gene 270:231-6 (2001)
DOI:10.1016/S0378-1119(01)00471-1
Reference
  Authors
Wang S, Czuchry D, Liu B, Vinnikova AN, Gao Y, Vlahakis JZ, Szarek WA, Wang L, Feng L, Brockhausen I
  Title
Characterization of two UDP-Gal:GalNAc-diphosphate-lipid beta1,3-galactosyltransferases WbwC from Escherichia coli serotypes O104 and O5.
  Journal
J Bacteriol 196:3122-33 (2014)
DOI:10.1128/JB.01698-14
Reference
  Authors
Czuchry D, Desormeaux P, Stuart M, Jarvis DL, Matta KL, Szarek WA, Brockhausen I
  Title
Identification and Biochemical Characterization of the Novel alpha2,3-Sialyltransferase WbwA from Pathogenic Escherichia coli Serotype O104.
  Journal
J Bacteriol 197:3760-8 (2015)
DOI:10.1128/JB.00521-15
Reference
  Authors
Czuchry D, Szarek WA, Brockhausen I
  Title
Identification and biochemical characterization of WbwB, a novel UDP-Gal: Neu5Ac-R alpha1,4-galactosyltransferase from the intestinal pathogen Escherichia  coli serotype O104.
  Journal
Glycoconj J 35:65-76 (2018)
DOI:10.1007/s10719-017-9799-y
Reference
  Authors
Stevenson G, Dieckelmann M, Reeves PR
  Title
Determination of glycosyltransferase specificities for the Escherichia coli O111 O antigen by a generic approach.
  Journal
Appl Environ Microbiol 74:1294-8 (2008)
DOI:10.1128/AEM.02660-07
Reference
PMID:9774562
  Authors
Wang L, Curd H, Qu W, Reeves PR
  Title
Sequencing of Escherichia coli O111 O-antigen gene cluster and identification of O111-specific genes.
  Journal
J Clin Microbiol 36:3182-7 (1998)
DOI:10.1128/JCM.36.11.3182-3187.1998
Reference
  Authors
Feng L, Senchenkova SN, Tao J, Shashkov AS, Liu B, Shevelev SD, Reeves PR, Xu J, Knirel YA, Wang L
  Title
Structural and genetic characterization of enterohemorrhagic Escherichia coli O145 O antigen and development of an O145 serogroup-specific PCR assay.
  Journal
J Bacteriol 187:758-64 (2005)
DOI:10.1128/JB.187.2.758-764.2005
Reference
  Authors
Gao Y, Liu B, Strum S, Schutzbach JS, Druzhinina TN, Utkina NS, Torgov VI, Danilov LL, Veselovsky VV, Vlahakis JZ, Szarek WA, Wang L, Brockhausen I
  Title
Biochemical characterization of WbdN, a beta1,3-glucosyltransferase involved in O-antigen synthesis in enterohemorrhagic Escherichia coli O157.
  Journal
Glycobiology 22:1092-102 (2012)
DOI:10.1093/glycob/cws081
Reference
PMID:9673232
  Authors
Wang L, Reeves PR
  Title
Organization of Escherichia coli O157 O antigen gene cluster and identification of its specific genes.
  Journal
Infect Immun 66:3545-51 (1998)
DOI:10.1128/IAI.66.8.3545-3551.1998
Reference
  Authors
Hong Y, Liu MA, Reeves PR
  Title
Progress in Our Understanding of Wzx Flippase for Translocation of Bacterial Membrane Lipid-Linked Oligosaccharide.
  Journal
J Bacteriol 200:e00154-17 (2018)
DOI:10.1128/JB.00154-17
Reference
  Authors
Liu B, Furevi A, Perepelov AV, Guo X, Cao H, Wang Q, Reeves PR, Knirel YA, Wang L, Widmalm G.
  Title
Structure and genetics of Escherichia coli O antigens.
  Journal
FEMS Microbiol Rev fuz028 (2019)
DOI:10.1093/femsre/fuz028
KO pathway
ko00542   
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