KEGG   PATHWAY: acd03030
acd03030                    Pathway                                
DNA replication - Acinetobacter oleivorans
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Genetic Information Processing; Replication and repair
Pathway map
acd03030  DNA replication

Acinetobacter oleivorans [GN:acd]
AOLE_14070  bifunctional ribonuclease HI/DNA polymerase III subunit epsilon [KO:K14159] [EC:]
AOLE_11360  DNA polymerase III DnaE [KO:K02337] [EC:]
AOLE_06955  DNA polymerase III, tau and gamma subunits [KO:K02343] [EC:]
AOLE_10850  hypothetical protein [KO:K02341] [EC:]
AOLE_16740  DNA polymerase III, delta subunit [KO:K02340] [EC:]
AOLE_18265  DNA polymerase III chi subunit, HolC family protein [KO:K02339] [EC:]
AOLE_00010  DNA polymerase III, beta subunit [KO:K02338] [EC:]
AOLE_06285  replicative DNA helicase [KO:K02314] [EC:]
AOLE_15495  DNA primase [KO:K02316] [EC:]
AOLE_00960  ssDNA-binding protein controls activity of RecBCD nuclease [KO:K03111]
AOLE_13575  rnhB; ribonuclease HII [KO:K03470] [EC:]
AOLE_16400  DNA polymerase I(POL I) [KO:K02335] [EC:]
AOLE_15665  DNA ligase, NAD-dependent [KO:K01972] [EC:]
Stillman B.
Smart machines at the DNA replication fork.
Cell 78:725-8 (1994)
Waga S, Stillman B.
The DNA replication fork in eukaryotic cells.
Annu Rev Biochem 67:721-51 (1998)
KO pathway

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