KEGG   PATHWAY: sce03450
Entry
sce03450                    Pathway                                
Name
Non-homologous end-joining - Saccharomyces cerevisiae (budding yeast)
Description
Nonhomologous end joining (NHEJ) eliminates DNA double-strand breaks (DSBs) by direct ligation. NHEJ involves binding of the KU heterodimer to double-stranded DNA ends, recruitment of DNA-PKcs (MRX complex in yeast), processing of ends, and recruitment of the DNA ligase IV (LIG4)-XRCC4 complex, which brings about ligation. A recent study shows that bacteria accomplish NHEJ using just two proteins (Ku and DNA ligase), whereas eukaryotes require many factors. NHEJ repairs DSBs at all stages of the cell cycle, bringing about the ligation of two DNA DSBs without the need for sequence homology, and so is error-prone.
Class
Genetic Information Processing; Replication and repair
Pathway map
sce03450  Non-homologous end-joining
sce03450

Other DBs
GO: 0006303
Organism
Saccharomyces cerevisiae (budding yeast) [GN:sce]
Gene
YMR284W  YKU70; ATP-dependent DNA helicase YKU70 [KO:K10884]
YMR106C  YKU80; ATP-dependent DNA helicase YKU80 [KO:K10885]
YOR005C  DNL4; DNA ligase (ATP) DNL4 [KO:K10777] [EC:6.5.1.1]
YNL250W  RAD50; MRX complex DNA-binding subunit [KO:K10866] [EC:3.6.-.-]
YMR224C  MRE11; MRX complex nuclease subunit [KO:K10865]
YDR369C  XRS2; Xrs2p [KO:K10868]
YCR014C  POL4; DNA-directed DNA polymerase IV [KO:K10981] [EC:2.7.7.7]
YKL113C  RAD27; multifunctional nuclease RAD27 [KO:K04799] [EC:3.1.-.-]
YGL090W  LIF1; Lif1p [KO:K10982]
YLR265C  NEJ1; Nej1p [KO:K10983]
Reference
  Authors
Gennery AR.
  Title
Primary immunodeficiency syndromes associated with defective DNA double-strand break repair.
  Journal
Br Med Bull 77-78:71-85 (2006)
DOI:10.1093/bmb/ldl006
Reference
  Authors
Zhang Y, Hefferin ML, Chen L, Shim EY, Tseng HM, Kwon Y, Sung P, Lee SE, Tomkinson AE.
  Title
Role of Dnl4-Lif1 in nonhomologous end-joining repair complex assembly and suppression of homologous recombination.
  Journal
Nat Struct Mol Biol 14:639-46 (2007)
DOI:10.1038/nsmb1261
Reference
  Authors
Wyman C, Kanaar R.
  Title
DNA double-strand break repair: all's well that ends well.
  Journal
Annu Rev Genet 40:363-83 (2006)
DOI:10.1146/annurev.genet.40.110405.090451
Reference
  Authors
Weller GR, Brandt VL, Roth DB.
  Title
Doing more with less in bacterial DNA repair.
  Journal
Nat Struct Mol Biol 11:1158-9 (2004)
DOI:10.1038/nsmb1204-1158
Reference
  Authors
Weller GR, Kysela B, Roy R, Tonkin LM, Scanlan E, Della M, Devine SK, Day JP, Wilkinson A, d'Adda di Fagagna F, Devine KM, Bowater RP, Jeggo PA, Jackson SP, Doherty AJ.
  Title
Identification of a DNA nonhomologous end-joining complex in bacteria.
  Journal
Science 297:1686-9 (2002)
DOI:10.1126/science.1074584
Reference
  Authors
Pitcher RS, Brissett NC, Doherty AJ.
  Title
Nonhomologous end-joining in bacteria: a microbial perspective.
  Journal
Annu Rev Microbiol 61:259-82 (2007)
DOI:10.1146/annurev.micro.61.080706.093354
KO pathway
ko03450   
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