{
	"name":"dku03016",
	"children":[
	{
		"name":"Eukaryotic type",
		"children":[
		{
			"name":"5'processing factors",
			"children":[
			{
				"name":"Ribonucrease P"
			}
			]
		},
		{
			"name":"3'processing and CCA adding factors",
			"children":[
			{
				"name":"3'processing factors"
			},
			{
				"name":"CCA adding factors",
				"children":[
				{
					"name":"Desku_1871 CBS domain containing protein\tK00974 cca; tRNA nucleotidyltransferase (CCA-adding enzyme) [EC:2.7.7.72 3.1.3.- 3.1.4.-]"
				}
				]
			}
			]
		},
		{
			"name":"tRNA spricing factors",
			"children":[
			{
				"name":"tRNA splicing endonuclease complex"
			},
			{
				"name":"tRNA splicing ligase complex",
				"children":[
				{
					"name":"Desku_1497 protein of unknown function UPF0027\tK14415 RTCB; tRNA-splicing ligase RtcB (3'-phosphate\/5'-hydroxy nucleic acid ligase) [EC:6.5.1.8]"
				}
				]
			},
			{
				"name":"Other tRNA splicing factors"
			}
			]
		},
		{
			"name":"tRNA transport factors",
			"children":[
			{
				"name":"tRNA export factors"
			},
			{
				"name":"tRNA import factors"
			}
			]
		},
		{
			"name":"Aminoacyl-tRNA synthetases (AARSs)",
			"children":[
			{
				"name":"Multi-aminoacyl-tRNA synthetase complex (MSC)",
				"children":[
				{
					"name":"Desku_2550 Prolyl-tRNA synthetase\tK01881 PARS; prolyl-tRNA synthetase [EC:6.1.1.15]"
				},
				{
					"name":"Desku_1190 Isoleucyl-tRNA synthetase\tK01870 IARS; isoleucyl-tRNA synthetase [EC:6.1.1.5]"
				},
				{
					"name":"Desku_0441 leucyl-tRNA synthetase\tK01869 LARS; leucyl-tRNA synthetase [EC:6.1.1.4]"
				},
				{
					"name":"Desku_0043 Methionyl-tRNA synthetase\tK01874 MARS; methionyl-tRNA synthetase [EC:6.1.1.10]"
				},
				{
					"name":"Desku_3468 Arginyl-tRNA synthetase\tK01887 RARS; arginyl-tRNA synthetase [EC:6.1.1.19]"
				},
				{
					"name":"Desku_3195 Lysyl-tRNA synthetase\tK04567 KARS; lysyl-tRNA synthetase, class II [EC:6.1.1.6]"
				},
				{
					"name":"Desku_2051 aspartyl-tRNA synthetase\tK01876 DARS2; aspartyl-tRNA synthetase [EC:6.1.1.12]"
				}
				]
			},
			{
				"name":"Other AARSs",
				"children":[
				{
					"name":"Desku_2025 alanyl-tRNA synthetase\tK01872 AARS; alanyl-tRNA synthetase [EC:6.1.1.7]"
				},
				{
					"name":"Desku_0638 threonyl-tRNA synthetase\tK01868 TARS; threonyl-tRNA synthetase [EC:6.1.1.3]"
				},
				{
					"name":"Desku_0012 Seryl-tRNA synthetase\tK01875 SARS; seryl-tRNA synthetase [EC:6.1.1.11]"
				},
				{
					"name":"Desku_3142 cysteinyl-tRNA synthetase\tK01883 CARS; cysteinyl-tRNA synthetase [EC:6.1.1.16]"
				},
				{
					"name":"Desku_0403 Valyl-tRNA synthetase\tK01873 VARS; valyl-tRNA synthetase [EC:6.1.1.9]"
				},
				{
					"name":"Desku_2052 Histidyl-tRNA synthetase\tK01892 HARS; histidyl-tRNA synthetase [EC:6.1.1.21]"
				},
				{
					"name":"Desku_0645 Phenylalanyl-tRNA synthetase alpha chain\tK01889 FARSA; phenylalanyl-tRNA synthetase alpha chain [EC:6.1.1.20]"
				},
				{
					"name":"Desku_0646 Phenylalanyl-tRNA synthetase beta chain\tK01890 FARSB; phenylalanyl-tRNA synthetase beta chain [EC:6.1.1.20]"
				},
				{
					"name":"Desku_0659 Tyrosyl-tRNA synthetase\tK01866 YARS; tyrosyl-tRNA synthetase [EC:6.1.1.1]"
				},
				{
					"name":"Desku_1869 tryptophanyl-tRNA synthetase\tK01867 WARS; tryptophanyl-tRNA synthetase [EC:6.1.1.2]"
				}
				]
			},
			{
				"name":"Trans-editing factors",
				"children":[
				{
					"name":"Desku_2056 D-tyrosyl-tRNA(Tyr) deacylase\tK07560 dtd; D-aminoacyl-tRNA deacylase [EC:3.1.1.96]"
				}
				]
			},
			{
				"name":"AARS associated factors",
				"children":[
				{
					"name":"Desku_2655 histidine triad (HIT) protein\tK02503 HINT1_2; histidine triad (HIT) family protein [EC:3.9.1.-]"
				}
				]
			}
			]
		},
		{
			"name":"tRNA modification factors",
			"children":[
			{
				"name":"Deaminases"
			},
			{
				"name":"Dihydorouridine synthases"
			},
			{
				"name":"Methylthiotransferase",
				"children":[
				{
					"name":"Desku_1329 (Dimethylallyl)adenosine tRNA methylthiotransferase miaB\tK06168 CDK5RAP1; tRNA-2-methylthio-N6-dimethylallyladenosine synthase [EC:2.8.4.3]"
				},
				{
					"name":"Desku_2656 RNA modification enzyme, MiaB family\tK15865 CDKAL1; threonylcarbamoyladenosine tRNA methylthiotransferase [EC:2.8.4.5]"
				}
				]
			},
			{
				"name":"Methyltransferases",
				"children":[
				{
					"name":"Desku_2668 tRNA (adenine-N(1)-)-methyltransferase\tK07442 TRM61; tRNA (adenine57-N1\/adenine58-N1)-methyltransferase catalytic subunit [EC:2.1.1.219 2.1.1.220]"
				}
				]
			},
			{
				"name":"Psudouridine synthases",
				"children":[
				{
					"name":"Desku_3024 tRNA pseudouridine synthase A\tK06173 truA; tRNA pseudouridine38-40 synthase [EC:5.4.99.12]"
				},
				{
					"name":"Desku_2540 tRNA pseudouridine synthase B\tK03177 truB; tRNA pseudouridine55 synthase [EC:5.4.99.25]"
				},
				{
					"name":"Desku_2863 tRNA pseudouridine synthase D TruD\tK06176 truD; tRNA pseudouridine13 synthase [EC:5.4.99.27]"
				}
				]
			},
			{
				"name":"Thiolation factors",
				"children":[
				{
					"name":"Desku_2031 cysteine desulfurase NifS\tK04487 iscS; cysteine desulfurase [EC:2.8.1.7]"
				}
				]
			},
			{
				"name":"KEOPS\/EKC complex"
			},
			{
				"name":"Elongator complex"
			},
			{
				"name":"Elongator related proteins"
			},
			{
				"name":"Other tRNA modification factors",
				"children":[
				{
					"name":"Desku_3442 Sua5\/YciO\/YrdC\/YwlC family protein\tK07566 tsaC; L-threonylcarbamoyladenylate synthase [EC:2.7.7.87]"
				},
				{
					"name":"Desku_1335 tRNA dimethylallyltransferase\tK00791 miaA; tRNA dimethylallyltransferase [EC:2.5.1.75]"
				},
				{
					"name":"Desku_3567 tRNA modification GTPase mnmE\tK03650 mnmE; tRNA modification GTPase [EC:3.6.-.-]"
				},
				{
					"name":"Desku_3566 tRNA uridine 5-carboxymethylaminomethyl modification enzyme mnmG\tK03495 gidA; tRNA uridine 5-carboxymethylaminomethyl modification enzyme"
				},
				{
					"name":"Desku_2639 Selenide, water dikinase\tK01008 selD; selenide, water dikinase [EC:2.7.9.3]"
				}
				]
			}
			]
		},
		{
			"name":"tRNA degradation factors",
			"children":[
			{
				"name":"TRAMP complex"
			},
			{
				"name":"Rapid tRNA decay (RTD) pathway factors"
			},
			{
				"name":"Other tRNA degradation factors"
			}
			]
		}
		]
	},
	{
		"name":"Prokaryotic type",
		"children":[
		{
			"name":"5'processing factors",
			"children":[
			{
				"name":"Ribonucrease P",
				"children":[
				{
					"name":"Desku_3571 Ribonuclease P protein component\tK03536 rnpA; ribonuclease P protein component [EC:3.1.26.5]"
				},
				{
					"name":"Desku_R0044 rnpB; RNA component of RNaseP\tK01978 rnpB; RNase P catalytic RNA component"
				}
				]
			}
			]
		},
		{
			"name":"3'processing and CCA adding factors",
			"children":[
			{
				"name":"3'processing factors",
				"children":[
				{
					"name":"Desku_0371 Ribonuclease PH\tK00989 rph; ribonuclease PH [EC:2.7.7.56]"
				}
				]
			},
			{
				"name":"CCA adding factors",
				"children":[
				{
					"name":"Desku_1871 CBS domain containing protein\tK00974 cca; tRNA nucleotidyltransferase (CCA-adding enzyme) [EC:2.7.7.72 3.1.3.- 3.1.4.-]"
				}
				]
			}
			]
		},
		{
			"name":"tRNA spricing factors",
			"children":[
			{
				"name":"tRNA splicing endonucleases"
			},
			{
				"name":"tRNA splicing ligases",
				"children":[
				{
					"name":"Desku_1497 protein of unknown function UPF0027\tK14415 RTCB; tRNA-splicing ligase RtcB (3'-phosphate\/5'-hydroxy nucleic acid ligase) [EC:6.5.1.8]"
				}
				]
			},
			{
				"name":"Other tRNA splicing factors",
				"children":[
				{
					"name":"Desku_2613 2'-5' RNA ligase\tK01975 thpR; RNA 2',3'-cyclic 3'-phosphodiesterase [EC:3.1.4.58]"
				}
				]
			}
			]
		},
		{
			"name":"tRNA transport factors",
			"children":[
			{
				"name":"tRNA export factors"
			}
			]
		},
		{
			"name":"Aminoacyl-tRNA synthetases (AARSs)",
			"children":[
			{
				"name":"Multi-aminoacyl-tRNA synthetase complex (MSC)",
				"children":[
				{
					"name":"Desku_0441 leucyl-tRNA synthetase\tK01869 LARS; leucyl-tRNA synthetase [EC:6.1.1.4]"
				},
				{
					"name":"Desku_2550 Prolyl-tRNA synthetase\tK01881 PARS; prolyl-tRNA synthetase [EC:6.1.1.15]"
				}
				]
			},
			{
				"name":"Other AARSs",
				"children":[
				{
					"name":"Desku_0659 Tyrosyl-tRNA synthetase\tK01866 YARS; tyrosyl-tRNA synthetase [EC:6.1.1.1]"
				},
				{
					"name":"Desku_1869 tryptophanyl-tRNA synthetase\tK01867 WARS; tryptophanyl-tRNA synthetase [EC:6.1.1.2]"
				},
				{
					"name":"Desku_0638 threonyl-tRNA synthetase\tK01868 TARS; threonyl-tRNA synthetase [EC:6.1.1.3]"
				},
				{
					"name":"Desku_1190 Isoleucyl-tRNA synthetase\tK01870 IARS; isoleucyl-tRNA synthetase [EC:6.1.1.5]"
				},
				{
					"name":"Desku_3195 Lysyl-tRNA synthetase\tK04567 KARS; lysyl-tRNA synthetase, class II [EC:6.1.1.6]"
				},
				{
					"name":"Desku_2025 alanyl-tRNA synthetase\tK01872 AARS; alanyl-tRNA synthetase [EC:6.1.1.7]"
				},
				{
					"name":"Desku_0403 Valyl-tRNA synthetase\tK01873 VARS; valyl-tRNA synthetase [EC:6.1.1.9]"
				},
				{
					"name":"Desku_0043 Methionyl-tRNA synthetase\tK01874 MARS; methionyl-tRNA synthetase [EC:6.1.1.10]"
				},
				{
					"name":"Desku_0012 Seryl-tRNA synthetase\tK01875 SARS; seryl-tRNA synthetase [EC:6.1.1.11]"
				},
				{
					"name":"Desku_2051 aspartyl-tRNA synthetase\tK01876 DARS2; aspartyl-tRNA synthetase [EC:6.1.1.12]"
				},
				{
					"name":"Desku_2619 Glycyl-tRNA synthetase alpha subunit\tK01878 glyQ; glycyl-tRNA synthetase alpha chain [EC:6.1.1.14]"
				},
				{
					"name":"Desku_2618 Glycyl-tRNA synthetase beta subunit\tK01879 glyS; glycyl-tRNA synthetase beta chain [EC:6.1.1.14]"
				},
				{
					"name":"Desku_3142 cysteinyl-tRNA synthetase\tK01883 CARS; cysteinyl-tRNA synthetase [EC:6.1.1.16]"
				},
				{
					"name":"Desku_3468 Arginyl-tRNA synthetase\tK01887 RARS; arginyl-tRNA synthetase [EC:6.1.1.19]"
				},
				{
					"name":"Desku_0645 Phenylalanyl-tRNA synthetase alpha chain\tK01889 FARSA; phenylalanyl-tRNA synthetase alpha chain [EC:6.1.1.20]"
				},
				{
					"name":"Desku_0646 Phenylalanyl-tRNA synthetase beta chain\tK01890 FARSB; phenylalanyl-tRNA synthetase beta chain [EC:6.1.1.20]"
				},
				{
					"name":"Desku_2052 Histidyl-tRNA synthetase\tK01892 HARS; histidyl-tRNA synthetase [EC:6.1.1.21]"
				},
				{
					"name":"Desku_3144 glutamyl-tRNA synthetase\tK09698 gltX; nondiscriminating glutamyl-tRNA synthetase [EC:6.1.1.24]"
				}
				]
			},
			{
				"name":"Trans-editing factors",
				"children":[
				{
					"name":"Desku_2056 D-tyrosyl-tRNA(Tyr) deacylase\tK07560 dtd; D-aminoacyl-tRNA deacylase [EC:3.1.1.96]"
				}
				]
			}
			]
		},
		{
			"name":"tRNA modification factors",
			"children":[
			{
				"name":"Deaminases",
				"children":[
				{
					"name":"Desku_0019 CMP\/dCMP deaminase zinc-binding protein\tK11991 tadA; tRNA(adenine34) deaminase [EC:3.5.4.33]"
				}
				]
			},
			{
				"name":"Dihydorouridine synthases",
				"children":[
				{
					"name":"Desku_3199 TIM-barrel protein, nifR3 family\tK05540 dusB; tRNA-dihydrouridine synthase B [EC:1.-.-.-]"
				}
				]
			},
			{
				"name":"Methylthiotransferases",
				"children":[
				{
					"name":"Desku_1329 (Dimethylallyl)adenosine tRNA methylthiotransferase miaB\tK06168 CDK5RAP1; tRNA-2-methylthio-N6-dimethylallyladenosine synthase [EC:2.8.4.3]"
				},
				{
					"name":"Desku_2656 RNA modification enzyme, MiaB family\tK15865 CDKAL1; threonylcarbamoyladenosine tRNA methylthiotransferase [EC:2.8.4.5]"
				}
				]
			},
			{
				"name":"Methyltransferases",
				"children":[
				{
					"name":"Desku_0988 Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO\tK04094 trmFO; methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase [EC:2.1.1.74]"
				},
				{
					"name":"Desku_2141 tRNA (guanine-N(1)-)-methyltransferase\tK00554 trmD; tRNA (guanine37-N1)-methyltransferase [EC:2.1.1.228]"
				},
				{
					"name":"Desku_3567 tRNA modification GTPase mnmE\tK03650 mnmE; tRNA modification GTPase [EC:3.6.-.-]"
				},
				{
					"name":"Desku_2598 protein of unknown function DUF633\tK06967 trmK; tRNA (adenine22-N1)-methyltransferase [EC:2.1.1.217]"
				},
				{
					"name":"Desku_1663 RNA methyltransferase, TrmH family, group 2\tK03216 trmL; tRNA (cytidine\/uridine-2'-O-)-methyltransferase [EC:2.1.1.207]"
				},
				{
					"name":"Desku_0644 RNA methyltransferase, TrmH family, group 3\tK03437 spoU; RNA methyltransferase, TrmH family"
				}
				]
			},
			{
				"name":"Psudouridine synthases",
				"children":[
				{
					"name":"Desku_3024 tRNA pseudouridine synthase A\tK06173 truA; tRNA pseudouridine38-40 synthase [EC:5.4.99.12]"
				},
				{
					"name":"Desku_2540 tRNA pseudouridine synthase B\tK03177 truB; tRNA pseudouridine55 synthase [EC:5.4.99.25]"
				},
				{
					"name":"Desku_2863 tRNA pseudouridine synthase D TruD\tK06176 truD; tRNA pseudouridine13 synthase [EC:5.4.99.27]"
				}
				]
			},
			{
				"name":"Thiolation factors",
				"children":[
				{
					"name":"Desku_2029 tRNA-specific 2-thiouridylase mnmA\tK00566 mnmA; tRNA-uridine 2-sulfurtransferase [EC:2.8.1.13]"
				},
				{
					"name":"Desku_0385 PP-loop domain protein\tK21947 ttuA; tRNA-5-methyluridine54 2-sulfurtransferase [EC:2.8.1.15]"
				},
				{
					"name":"Desku_2304 PP-loop domain protein\tK14058 ttcA; tRNA 2-thiocytidine biosynthesis protein TtcA"
				},
				{
					"name":"Desku_3089 PP-loop domain protein\tK14058 ttcA; tRNA 2-thiocytidine biosynthesis protein TtcA"
				},
				{
					"name":"Desku_0300 SirA-like domain-containing protein\tK04085 tusA; tRNA 2-thiouridine synthesizing protein A [EC:2.8.1.-]"
				},
				{
					"name":"Desku_2031 cysteine desulfurase NifS\tK04487 iscS; cysteine desulfurase [EC:2.8.1.7]"
				}
				]
			},
			{
				"name":"KEOPS\/EKC complex"
			},
			{
				"name":"Other tRNA modification factors",
				"children":[
				{
					"name":"Desku_3566 tRNA uridine 5-carboxymethylaminomethyl modification enzyme mnmG\tK03495 gidA; tRNA uridine 5-carboxymethylaminomethyl modification enzyme"
				},
				{
					"name":"Desku_1335 tRNA dimethylallyltransferase\tK00791 miaA; tRNA dimethylallyltransferase [EC:2.5.1.75]"
				},
				{
					"name":"Desku_0671 S-adenosylmethionine:tRNA ribosyltransferase-isomerase\tK07568 queA; S-adenosylmethionine:tRNA ribosyltransferase-isomerase [EC:2.4.99.17]"
				},
				{
					"name":"Desku_1422 exsB protein\tK06920 queC; 7-cyano-7-deazaguanine synthase [EC:6.3.4.20]"
				},
				{
					"name":"Desku_1423 queuosine biosynthesis protein QueD\tK01737 queD; 6-pyruvoyltetrahydropterin\/6-carboxytetrahydropterin synthase [EC:4.2.3.12 4.1.2.50]"
				},
				{
					"name":"Desku_1420 Radical SAM domain protein\tK10026 queE; 7-carboxy-7-deazaguanine synthase [EC:4.3.99.3]"
				},
				{
					"name":"Desku_0061 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein\tK18979 queG; epoxyqueuosine reductase [EC:1.17.99.6]"
				},
				{
					"name":"Desku_0309 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein\tK18979 queG; epoxyqueuosine reductase [EC:1.17.99.6]"
				},
				{
					"name":"Desku_0668 protein of unknown function DUF208\tK09765 queH; epoxyqueuosine reductase [EC:1.17.99.6]"
				},
				{
					"name":"Desku_2639 Selenide, water dikinase\tK01008 selD; selenide, water dikinase [EC:2.7.9.3]"
				},
				{
					"name":"Desku_2391 tRNA 2-selenouridine synthase\tK06917 selU; tRNA 2-selenouridine synthase [EC:2.9.1.3]"
				},
				{
					"name":"Desku_0672 Queuine tRNA-ribosyltransferase\tK00773 tgt; queuine tRNA-ribosyltransferase [EC:2.4.2.29]"
				},
				{
					"name":"Desku_0212 tRNA(Ile)-lysidine synthase\tK04075 tilS; tRNA(Ile)-lysidine synthase [EC:6.3.4.19]"
				},
				{
					"name":"Desku_0547 universal protein YeaZ\tK14742 tsaB; tRNA threonylcarbamoyladenosine biosynthesis protein TsaB"
				},
				{
					"name":"Desku_3442 Sua5\/YciO\/YrdC\/YwlC family protein\tK07566 tsaC; L-threonylcarbamoyladenylate synthase [EC:2.7.7.87]"
				},
				{
					"name":"Desku_3001 O-sialoglycoprotein endopeptidase\tK25706 tsaD; tRNA N6-adenosine threonylcarbamoyltransferase [EC:2.3.1.234]"
				},
				{
					"name":"Desku_0546 Uncharacterized protein family UPF0079, ATPase\tK06925 tsaE; tRNA threonylcarbamoyladenosine biosynthesis protein TsaE"
				},
				{
					"name":"Desku_2050 UBA\/THIF-type NAD\/FAD binding protein\tK22132 tcdA; tRNA threonylcarbamoyladenosine dehydratase"
				}
				]
			}
			]
		},
		{
			"name":"tRNA degradation factors",
			"children":[
			{
				"name":"Desku_2535 Polyribonucleotide nucleotidyltransferase\tK00962 pnp; polyribonucleotide nucleotidyltransferase [EC:2.7.7.8]"
			}
			]
		}
		]
	}
	]
}