Upload query amino acid sequences in FASTA format
Enter FASTA sequences

Or upload file:

Select GENES family/genus dataset to be searched
   Query data: sequence.txt  
   Select Buchnera (32199)

Annotate Sequence is an interface to the BlastKOALA server for assigning KO identifiers (K numbers) to a given sequence data set for subsequent analyses with Reconstruct Pathway, Reconstruct Brite, and Reconstruct Module tools. The BlastKOALA computation is performed in an interactive mode using an appropriate subset of KEGG GENES (corresponding to family/genus of your organism). This tool is useful for converting your sequence IDs to K numbers.

Query sequences – Upload a file containing multiple amino acid sequences represented by the FASTA format. The maximum number of sequences is 2500.

Database sequences – Use the Family/Genus button to enter the NCBI taxonomy number for an organism group at the family level for eukaryotes or at the genus level for prokaryotes. The corresponding KEGG GENES sequences are used as database sequences.

Annotation result – The result page contains links to KEGG Mapper analyses as well as to viewing and downloading the K number assignment list.

If you have a larger dataset of query sequences, consider splitting the dataset and concatenating the lists of K number assignments that can be downloaded from the result page. The BlastKOALA server accessed from its main page allows you to compare against all KEGG organisms as well as to use a better scoring scheme incorporating Pfam domains.

Last updated: May 11, 2015