KEGG   ORTHOLOGY: K15800
Entry
K15800                      KO                                     
Symbol
GAO
Name
germacrene A oxidase [EC:1.14.14.95]
Pathway
map00909  Sesquiterpenoid and triterpenoid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R09548  germacra-1(10),4,11(13)-trien-12-ol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase
R09549  (+)-germacrene-A,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (12-hydroxylating)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00909 Sesquiterpenoid and triterpenoid biosynthesis
    K15800  GAO; germacrene A oxidase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.95  germacrene A hydroxylase
     K15800  GAO; germacrene A oxidase
Other DBs
GO: 0102260
Genes
HAN: 110898214
LSV: 111882413 111906166 111906167
CCAV: 112515273 112515404 112515606
AG: ADF32078(GAO1) ADF43080(CiGAO)
Reference
PMID:20351109
  Authors
Nguyen DT, Gopfert JC, Ikezawa N, Macnevin G, Kathiresan M, Conrad J, Spring O, Ro DK
  Title
Biochemical conservation and evolution of germacrene A oxidase in asteraceae.
  Journal
J Biol Chem 285:16588-98 (2010)
DOI:10.1074/jbc.M110.111757
  Sequence
[ag:ADF43080 ADF32078]
LinkDB

KEGG   ENZYME: 1.14.13.123
Entry
EC 1.14.13.123    Obsolete  Enzyme                                 
Name
Transferred to 1.14.14.95
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Comment
Transferred entry: germacrene A hydroxylase. Now EC 1.14.14.95, germacrene A hydroxylase
History
EC 1.14.13.123 created 2011, deleted 2018
LinkDB

KEGG   REACTION: R09549
Entry
R09549                      Reaction                               
Name
(+)-germacrene-A,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (12-hydroxylating)
Definition
(+)-Germacrene A + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> Germacra-1(10),4,11(13)-trien-12-ol + [Oxidized NADPH---hemoprotein reductase] + H2O
Equation
C16141 + C03024 + C00007 <=> C19676 + C03161 + C00001
Comment
the first step of three-step reaction (see R12128, R09549+R12129+R12130)
Reaction class
RC01137  C16141_C19676
Enzyme
1.14.14.95
Pathway
rn00909  Sesquiterpenoid and triterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.95
     R09549  (+)-Germacrene A + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> Germacra-1(10),4,11(13)-trien-12-ol + [Oxidized NADPH---hemoprotein reductase] + H2O
Orthology
K15800  germacrene A oxidase [EC:1.14.14.95]
Other DBs
RHEA: 61311
LinkDB

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