KEGG   ORTHOLOGY: K15807
Entry
K15807            Tight     KO                                     
Symbol
TPS177
Name
patchoulol/alpha-guaiene/delta-guaiene synthase [EC:4.2.3.70 4.2.3.87 4.2.3.93]
Pathway
map00909  Sesquiterpenoid and triterpenoid biosynthesis
Reaction
R09629  (2E,6E)-farnesyl-diphosphate diphosphate-lyase (patchoulol-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00909 Sesquiterpenoid and triterpenoid biosynthesis
    K15807  TPS177; patchoulol/alpha-guaiene/delta-guaiene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.70  patchoulol synthase
     K15807  TPS177; patchoulol/alpha-guaiene/delta-guaiene synthase
    4.2.3.87  alpha-guaiene synthase
     K15807  TPS177; patchoulol/alpha-guaiene/delta-guaiene synthase
    4.2.3.93  delta-guaiene synthase
     K15807  TPS177; patchoulol/alpha-guaiene/delta-guaiene synthase
Genes
AG: AAS86323
Reference
PMID:2171435
  Authors
Munck SL, Croteau R
  Title
Purification and characterization of the sesquiterpene cyclase patchoulol synthase from Pogostemon cablin.
  Journal
Arch Biochem Biophys 282:58-64 (1990)
DOI:10.1016/0003-9861(90)90086-E
  Sequence
[ag:AAS86323]
Reference
PMID:16970904
  Authors
Deguerry F, Pastore L, Wu S, Clark A, Chappell J, Schalk M
  Title
The diverse sesquiterpene profile of patchouli, Pogostemon cablin, is correlated with a limited number of sesquiterpene synthases.
  Journal
Arch Biochem Biophys 454:123-36 (2006)
DOI:10.1016/j.abb.2006.08.006
  Sequence
[ag:AAS86323]
LinkDB

KEGG   ENZYME: 4.2.3.70
Entry
EC 4.2.3.70                 Enzyme                                 
Name
patchoulol synthase
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (patchoulol-forming)
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate + H2O = patchoulol + diphosphate [RN:R09629]
Reaction(KEGG)
R09629
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448];
H2O [CPD:C00001]
Product
patchoulol [CPD:C19755];
diphosphate [CPD:C00013]
History
EC 4.2.3.70 created 2011
Pathway
ec00909  Sesquiterpenoid and triterpenoid biosynthesis
Orthology
K15807  patchoulol/alpha-guaiene/delta-guaiene synthase
Reference
1  [PMID:3038029]
  Authors
Croteau R, Munck SL, Akoh CC, Fisk HJ, Satterwhite DM
  Title
Biosynthesis of the sesquiterpene patchoulol from farnesyl pyrophosphate in leaf  extracts of Pogostemon cablin (patchouli): mechanistic considerations.
  Journal
Arch Biochem Biophys 256:56-68 (1987)
DOI:10.1016/0003-9861(87)90425-5
  Sequence
[ag:AAS86323]
Reference
2  [PMID:2171435]
  Authors
Munck SL, Croteau R
  Title
Purification and characterization of the sesquiterpene cyclase patchoulol synthase from Pogostemon cablin.
  Journal
Arch Biochem Biophys 282:58-64 (1990)
DOI:10.1016/0003-9861(90)90086-E
  Sequence
[ag:AAS86323]
Reference
3  [PMID:20148554]
  Authors
Faraldos JA, Wu S, Chappell J, Coates RM
  Title
Doubly deuterium-labeled patchouli alcohol from cyclization of singly labeled [2-(2)H(1)]farnesyl diphosphate catalyzed by recombinant patchoulol synthase.
  Journal
J Am Chem Soc 132:2998-3008 (2010)
DOI:10.1021/ja909251r
  Sequence
[ag:AAS86323]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.70
IUBMB Enzyme Nomenclature: 4.2.3.70
ExPASy - ENZYME nomenclature database: 4.2.3.70
BRENDA, the Enzyme Database: 4.2.3.70
LinkDB

KEGG   REACTION: R09629
Entry
R09629                      Reaction                               
Name
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (patchoulol-forming)
Definition
trans,trans-Farnesyl diphosphate + H2O <=> Patchoulol + Diphosphate
Equation
C00448 + C00001 <=> C19755 + C00013
Reaction class
RC02591  C00448_C19755
Enzyme
4.2.3.70
Pathway
rn00909  Sesquiterpenoid and triterpenoid biosynthesis
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.70
     R09629  trans,trans-Farnesyl diphosphate + H2O <=> Patchoulol + Diphosphate
Orthology
K15807  patchoulol/alpha-guaiene/delta-guaiene synthase [EC:4.2.3.70 4.2.3.87 4.2.3.93]
Other DBs
RHEA: 29506
LinkDB

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