KEGG   ENZYME: 1.14.13.237
Entry
EC 1.14.13.237              Enzyme                                 
Name
aliphatic glucosinolate S-oxygenase;
omega-(methylthio)alkylglucosinolate S-oxygenase;
GS-OX1 (gene name);
omega-(methylthio)alkyl-glucosinolate,NADPH:oxygen S-oxidoreductase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
omega-(methylsulfanyl)alkyl-glucosinolate,NADPH:oxygen S-oxidoreductase
Reaction(IUBMB)
an omega-(methylsulfanyl)alkyl-glucosinolate + NADPH + H+ + O2 = an omega-(methylsulfinyl)alkyl-glucosinolate + NADP+ + H2O [RN:R11804]
Reaction(KEGG)
R11804
Substrate
omega-(methylsulfanyl)alkyl-glucosinolate [CPD:C21691];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
omega-(methylsulfinyl)alkyl-glucosinolate [CPD:C21692];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme is a member of the flavin-dependent monooxygenase (FMO) family (cf. EC 1.14.13.8). The plant Arabidopsis thaliana contains five isoforms. GS-OX1 through GS-OX4 are able to catalyse the S-oxygenation independent of chain length, while GS-OX5 is specific for 8-(methylsulfanyl)octyl glucosinolate.
History
EC 1.14.13.237 created 2017
Orthology
K22324  aliphatic glucosinolate S-oxygenase
Genes
ATH: AT1G12140(FMO_GS-OX5) AT1G62540(FMO_GS-OX2) AT1G62560(FMO_GS-OX3) AT1G62570(FMO_GS-OX4) AT1G65860(FMO_GS-OX1)
ALY: 9324080 9324081 9324082 9325981
CRB: 17894434 17895404 17898033 17900728
CSAT: 104703860 104703861 104714592 104714593 104714594 104739865 104739867 104739868 104752912 104752914 104755480 104771010
EUS: EUTSA_v10007582mg EUTSA_v10023450mg EUTSA_v10023453mg
BRP: 103836177 103838386(FMOGS-OX1) 103843151
BNA: 106349044 106362185 106382400 106401463 106415065
BOE: 106311680 106312190 106318728
RSZ: 108820983 108825620 108825621 108837259
Reference
1  [PMID:17461789]
  Authors
Hansen BG, Kliebenstein DJ, Halkier BA
  Title
Identification of a flavin-monooxygenase as the S-oxygenating enzyme in aliphatic glucosinolate biosynthesis in Arabidopsis.
  Journal
Plant J 50:902-10 (2007)
DOI:10.1111/j.1365-313X.2007.03101.x
  Sequence
[ath:AT1G65860]
Reference
2  [PMID:18799661]
  Authors
Li J, Hansen BG, Ober JA, Kliebenstein DJ, Halkier BA
  Title
Subclade of flavin-monooxygenases involved in aliphatic glucosinolate biosynthesis.
  Journal
Plant Physiol 148:1721-33 (2008)
DOI:10.1104/pp.108.125757
  Sequence
[ath:AT1G65860]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.237
IUBMB Enzyme Nomenclature: 1.14.13.237
ExPASy - ENZYME nomenclature database: 1.14.13.237
BRENDA, the Enzyme Database: 1.14.13.237
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