Entry
Name
multisite-specific tRNA:(cytosine-C5)-methyltransferase;
multisite-specific tRNA:m5C-methyltransferase;
TRM4 (gene name, gene corresponding to ORF YBL024w)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:tRNA (cytosine-C5)-methyltransferase
Reaction(IUBMB)
(1) S-adenosyl-L-methionine + cytosine34 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor [RN:
R00595 ];
(2) S-adenosyl-L-methionine + cytosine40 in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor;
(3) S-adenosyl-L-methionine + cytosine48 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA;
(4) S-adenosyl-L-methionine + cytosine49 in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:
C00019 ];
cytosine34 in tRNA precursor;
cytosine40 in tRNA precursor;
cytosine48 in tRNA;
cytosine49 in tRNA
Product
S-adenosyl-L-homocysteine [CPD:
C00021 ];
5-methylcytosine34 in tRNA precursor;
5-methylcytosine40 in tRNA precursor;
5-methylcytosine48 in tRNA;
5-methylcytosine49 in tRNA
Comment
The enzyme from Saccharomyces cerevisiae is responsible for complete 5-methylcytosine methylations of yeast tRNA. The incidence of modification depends on the cytosine position in tRNA. At positions 34 and 40, 5-methylcytosine is found only in two yeast tRNAs (tRNALeu(CUA) and tRNAPhe(GAA), respectively), whereas most other elongator yeast tRNAs bear either 5-methylcytosine48 or 5-methylcytosine49, but never both in the same tRNA molecule [1]. The formation of 5-methylcytosine34 and 5-methylcytosine40 is a strictly intron-dependent process, whereas the formation of 5-methylcytosine48 and 5-methylcytosine49 is an intron-independent process [2,3].
History
EC 2.1.1.202 created 1976 as EC 2.1.1.29, part transferred 2011 to EC 2.1.1.202
Orthology
K15334 multisite-specific tRNA:(cytosine-C5)-methyltransferase
Genes
CSAT : 104702776 104713682 104720928 104751878
EUS : EUTSA_v10000050mg EUTSA_v10024482mg
BRP : 103837897 103854554 103864345
BNA : 106363702 106406275 106415936 106439372 106446409 111215813
BOE : 106292165 106312686 106342432
RSZ : 108813377 108839838 108855463
HSYR : 120122418 120195582
GSJ : 114382634 114413089 114421850
PVU : PHAVU_001G253400g PHAVU_002G261300g
VUM : 124825441 124825443 124828169
CCAJ : 109798068 109813507
APRC : 113849527 113873888
TPRA : 123906248 123908167 123917005
PSAT : 127082943 127096483 127107671
VVO : 131603250 131661462 131661472
LJA : Lj0g3v0259609.1(Lj0g3v0259609.1) Lj6g3v0550640.1(Lj6g3v0550640.1) Lj6g3v0550640.2(Lj6g3v0550640.2) Lj6g3v0550640.3(Lj6g3v0550640.3)
LANG : 109343679 109361180 109362630
PCIN : 129309217 129320546
QSA : O6P43_023175 O6P43_030487
CMAX : 111478413 111496800
CMOS : 111453515 111463320
CPEP : 111781403 111803463
CAVE : 132165871 132166529
SPEN : 107004815 107020788
SSTN : 125845188 125867306
SDUL : 129880797 129898434
CANN : 107850803 107870980
NTA : 107766522 107802160 107808849 107822797
SSPL : 121768532 121772257
PSOM : 113286760 113312090 113343297 113357906
DOSA : Os08t0484400-01(Os08g0484400) Os09t0471900-01(Os09g0471900)
TDC : 119286785 119294937 119301908 119310338
TAES : 123086996 123090971 123095993 123104052 123112276 123121800
LPER : 127301950 127323960
LRD : 124653455 124657109 124685168
SITA : 101758722 101760456
PVIR : 120660973 120678763 120681975 120712694
PHAI : 112879702 112897214
SMO : SELMODRAFT_120643 SELMODRAFT_418525
OLU : OSTLU_27152 OSTLU_31007 OSTLU_36629
MPP : MICPUCDRAFT_45780(JGI:MicpuC2_45780)
NCS : NCAS_0B05640(NCAS0B05640)
NDI : NDAI_0B01830(NDAI0B01830) NDAI_0B02980(NDAI0B02980)
TPF : TPHA_0C02870(TPHA0C02870) TPHA_0H00150(TPHA0H00150)
TBL : TBLA_0A08740(TBLA0A08740)
TDL : TDEL_0B04390(TDEL0B04390)
KAF : KAFR_0B02220(KAFR0B02220)
KNG : KNAG_0D02050(KNAG0D02050) KNAG_0G01510(KNAG0G01510)
CAL : CAALFM_CR04300WA(CaO19.518)
NTE : NEUTE1DRAFT80343(NEUTE1DRAFT_80343)
BFU : BCIN_06g07260(Bcncl1)
SPO : SPAC17D4.04 SPAC23C4.17
SOM : SOMG_01788(trm402) SOMG_03864(trm401)
CCAC : CcaHIS019_0705920(NCL1)
SCM : SCHCO_02512227(SCHCODRAFT_02512227)
EIV : EIN_183240 EIN_373320
PTM : GSPATT00013766001 GSPATT00018463001
» show all
Taxonomy
Reference
Authors
Motorin Y, Grosjean H
Title
Multisite-specific tRNA:m5C-methyltransferase (Trm4) in yeast Saccharomyces cerevisiae: identification of the gene and substrate specificity of the enzyme.
Journal
Sequence
Reference
Authors
Jiang HQ, Motorin Y, Jin YX, Grosjean H
Title
Pleiotropic effects of intron removal on base modification pattern of yeast tRNAPhe: an in vitro study.
Journal
Reference
Authors
Strobel MC, Abelson J
Title
Effect of intron mutations on processing and function of Saccharomyces cerevisiae SUP53 tRNA in vitro and in vivo.
Journal
Reference
Authors
Walbott H, Husson C, Auxilien S, Golinelli-Pimpaneau B
Title
Cysteine of sequence motif VI is essential for nucleophilic catalysis by yeast tRNA m5C methyltransferase.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 2.1.1.202
LinkDB
All DBs