KEGG   ENZYME: 2.6.1.94
Entry
EC 2.6.1.94                 Enzyme                                 
Name
2'-deamino-2'-hydroxyneamine transaminase;
kacL (gene name)
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
Sysname
2'-deamino-2'-hydroxyneamine:2-oxoglutarate aminotransferase
Reaction(IUBMB)
2'-deamino-2'-hydroxyneamine + 2-oxoglutarate = 2'-deamino-2'-hydroxy-6'-dehydroparomamine + L-glutamate [RN:R10094]
Reaction(KEGG)
R10094;
(other) R11269 R11270
Substrate
2'-deamino-2'-hydroxyneamine [CPD:C20350];
2-oxoglutarate [CPD:C00026]
Product
2'-deamino-2'-hydroxy-6'-dehydroparomamine [CPD:C20351];
L-glutamate [CPD:C00025]
Comment
The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathway of kanamycin A and kanamycin D. The enzyme, characterized from the bacterium Streptomyces kanamyceticus, can also catalyse EC 2.6.1.93, neamine transaminase.
History
EC 2.6.1.94 created 2012
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K20568  neamine transaminase / 2'-deamino-2'-hydroxyneamine transaminase
Reference
1  [PMID:21983602]
  Authors
Park JW, Park SR, Nepal KK, Han AR, Ban YH, Yoo YJ, Kim EJ, Kim EM, Kim D, Sohng JK, Yoon YJ
  Title
Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation.
  Journal
Nat Chem Biol 7:843-52 (2011)
DOI:10.1038/nchembio.671
  Sequence
[ag:CAF60533]
Other DBs
ExplorEnz - The Enzyme Database: 2.6.1.94
IUBMB Enzyme Nomenclature: 2.6.1.94
ExPASy - ENZYME nomenclature database: 2.6.1.94
BRENDA, the Enzyme Database: 2.6.1.94
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