Hydrolases;
Acting on ester bonds;
Phosphoric-diester hydrolases
Sysname
oligonucleotide 5'-nucleotidohydrolase
Reaction(IUBMB)
Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides
Comment
Hydrolyses both ribonucleotides and deoxyribonucleotides. Has low activity towards polynucleotides. A 3'-phosphate terminus on the substrate inhibits hydrolysis.