KEGG ENZYME: 3.2.1.225

ENZYME: 3.2.1.225

Entry

EC 3.2.1.225                Enzyme

Name

D-arabinan exo alpha-(1,3)/(1,5)-arabinofuranosidase (non-reducing end);
exo-alpha-D-arabinofuranosidase;
DgGH172a;
DgGH172b;
DgGH172c;
NocGH172;
MycGH172;
ExoMA1

Class

Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

Sysname

alpha-D-arabinofuranoside non-reducing end alpha-D-arabinofuranosidase (configuration-retaining)

Reaction(IUBMB)

Hydrolysis of terminal non-reducing alpha-D-arabinofuranoside residues in D-arabinans

Comment

The enzyme hydrolyses alpha-D-arabinofuranosides with (1->3)- and (1->5)-linkages in D-arabinan core structure of lipoarabinomannan and arabinogalactan of mycobacterial cell wall. cf. EC 3.2.1.55, non-reducing end alpha-L-arabinofuranosidase; EC 3.2.1.224, D-arabinan exo beta-(1,2)-arabinofuranosidase (non-reducing end); and EC 3.2.1.226, D-arabinan endo alpha-(1,5)-arabinofuranosidase.

History

EC 3.2.1.225 created 2024

Orthology

K27494

D-arabinan exo alpha-(1,3)/(1,5)-arabinofuranosidase (non-reducing end)

Genes

NVE:

5511766

ADF:	107350488 107354230

AMIL:

114968589 114968675 114970562

DGT:

114517388

XEN:	124454523 124455676

HMG:

100205240

HSY:

130623386

AQU:	109581954 109591276

PGU:	PGUG_03952

TTT:	THITE_2118009

TMN:	UCRPA7_5218

SSCK:

SPSK_02518

FOX:	FOXG_17518

NHE:	NECHADRAFT_48881

FFC:	NCS54_01193800

FKR:	NCS57_01185300

PCHM:

VFPPC_10672 VFPPC_14047

PLJ:	VFPFJ_02986 VFPFJ_10270 VFPFJ_10464

PTKZ:

JDV02_008174 JDV02_008270

CFJ:	CFIO01_13533

CLUP:

CLUP02_01757

ANI:	ANIA_07098

AFM:	AFUA_5G00510

NFI:	NFIA_042000

ANG:	An06g02400

PCS:	N7525_011241

TRG:	TRUGW13939_07069

MBR:	MONBRDRAFT_21825

KOC:	AB185_28505

KLL:	BJF97_07975

LAZ:	A8A57_04775

SUPE:

P0H77_06030

CARU:

P0E69_21905

NIG:	C1N62_04155

XBC:	ELE36_17335

WOC:	BA177_04515 BA177_05115

EMP:	EZMO1_0729

GAP:	GAPWK_2250

GAQ:	RAM11_03685

FPP:	FPB0191_00080

VEI:

Veis_0526

MOP:	Mesop_5939

SME:

SM_b20234

SMK:	Sinme_3939

SMQ:	SinmeB_4436

SMX:	SM11_pD1377

SMI:	BN406_06432

SMEG:

C770_GR4pD1363

SMEL:

SM2011_b20234

SMER:

DU99_32200

SMD:

Smed_2312

EAK:	EKH55_4575

EMX:	FKV68_26695

ENU:	PYH37_001709 PYH37_001714

EGI:	PZN02_004225 PZN02_004728

STEG:

QA637_25420

RHL:	LPU83_pLPU83d1860

RJG:	CCGE525_09395

RHR:	CKA34_31565

BJU:	BJ6T_59890

BJP:	RN69_29015

BRS:

S23_43790

BRC:	BCCGELA001_16085

BOT:	CIT37_34635

BGQ:	X265_24025

BGZ:	XH91_21230

BDG:	LPJ38_21460

BBAN:

J4G43_021325

BCOU:

IC761_21785

BXN:	I3J27_15330

BRAZ:

LRP30_16705

LNE:	FZC33_26280

DEQ:	XM25_03865

DEI:	C4375_11760

DSAL:

K1X15_18785

DNP:	N8A98_16310

DAG:	PSQ19_05350

DRP:	PSQ90_10580

YTI:	FNA67_07700

AALA:

IGS74_14265

PPAF:

I8N54_17080

SPHC:

CVN68_03030

QCI:	NCF85_08035

SKT:	IGS68_27525

SMUC:

JL100_029625

SROE:

JL101_028415

KOZ:	KFF44_12880

GSO:	PH603_07325

DOL:

Dole_0956

SCL:

sce3321

SCU:	SCE1572_50515

PAUU:

E8A73_021970

MRM:	A7982_05151

SAMY:

DB32_003035

LLU:	AKJ09_07871

BACQ:

DOE78_08835

BCIR:

C2I06_01315

OON:	NP440_18020

GRC:	GI584_19230

MEKU:

HUW50_15430

BAG:

Bcoa_1661

ALKG:

MOJ78_02695

PJD:	Pjdr2_5449 Pjdr2_5939

GYM:	GYMC10_2578 GYMC10_2589 GYMC10_3781 GYMC10_3788

PTA:	HPL003_01845

PSAB:

PSAB_20120

PDU:	PDUR_22770

PAEN:

P40081_19380 P40081_19415 P40081_29695

PBJ:	VN24_25605 VN24_25805

PRI:	PRIO_4903 PRIO_4911

PSON:

JI735_10065 JI735_32190

POW:	IJ21_02160

PBV:	AR543_03795

PSWU:

SY83_08675

PIB:	BBD41_01885 BBD41_01990

PLW:	D5F53_11295

PPSC:

EHS13_24155 EHS13_24160 EHS13_24225 EHS13_26480 EHS13_26560 EHS13_26825 EHS13_27685

PALB:

EJC50_00400 EJC50_06005 EJC50_16415 EJC50_21645 EJC50_29225

PCHI:

PC41400_10720

PRZ:	GZH47_07935 GZH47_08785 GZH47_08840 GZH47_13600 GZH47_19400 GZH47_20110 GZH47_20155 GZH47_28645 GZH47_31170

PLYC:

GXP70_03030 GXP70_08460 GXP70_09680 GXP70_19010 GXP70_27185

PSOP:

KP014_26710

PTRI:

KDC22_17045 KDC22_17080

PAMY:

P9222_21140

PDY:	QJQ58_06090

PMAE:

LMZ02_19240 LMZ02_19295

PSPN:

L1F29_01655 L1F29_09235 L1F29_11755 L1F29_16730 L1F29_18430

PAUN:

MJA45_13045 MJA45_18145

PHK:	SK066_05775

PPAB:

KET34_05875 KET34_05890

COH:	EAV92_13340 EAV92_13410

CHEB:

HH215_01890 HH215_08560 HH215_12770 HH215_14265 HH215_29595 HH215_29630

NTR:	B0W44_12555 B0W44_12600

LAPI:

DKL56_01035 DKL56_07400

LPAI:

GYM71_01735 GYM71_09800

LHW:	BSQ49_12450

LBM:	DS830_06095

LDX:	LH506_03380

EFC:	EFAU004_00682

EFAU:

EFAU085_00630

EFU:	HMPREF0351_10291 HMPREF0351_10708

EAV:	EH197_03140 EH197_08865 EH197_19965

ESG:	EsVE80_18350

FCT:	NRE15_05065 NRE15_12885

CBE:

Cbei_3498

CBZ:

Cbs_3498

CDY:	F3K33_17730

CNN:

CNEO_1561

CTHD:

CDO33_03955

FSA:	C5Q98_04310 C5Q98_06890 C5Q98_06900

RUK:	A4V00_03570

FPRA:

CG447_03065

FDU:	GXM22_05830

AMUR:

ADH66_05430

RIX:

RO1_04400

RIM:

ROI_12140

ROB:	CK5_01060 CK5_01120

BYL:	A4V09_21455

BPRO:

PMF13cell1_00339 PMF13cell1_01147 PMF13cell1_01861

BLAB:

EYS05_11350

BLIQ:

INP51_02665 INP51_02940 INP51_02970

BWX:	NQ550_04860

BMAS:

LK422_12320

BLAR:

LC508_14615 LC508_20855 LC508_21830

BLUI:

Blut17040_05320

BCOC:

BLCOC_09170 BLCOC_51030 BLCOC_53430

CSCI:

HDCHBGLK_03487

LUA:	D4A81_00205

LGG:	QJR73_13160

ANR:	Ana3638_02555 Ana3638_09795

ACHT:

bsdcttw_21700

RTO:

RTO_14380

RGN:	RGna_09050

DLO:	K5I24_09115

MFOR:

NQ534_12810

ERE:	EUBREC_0286 EUBREC_3517

ERT:

EUR_31840

ERA:	ERE_00380 ERE_19410

CBIL:

EUBC25_02650

TTO:	Thethe_00435

MAS:	Mahau_0700

HALS:

D7D81_18150 D7D81_18155

IFN:	GM661_14770 GM661_14775

CMIT:

NQ499_10270

LPIL:

LIP_2038

MPA:

MAP_0339c

MAO:

MAP4_3531

MAVI:

RC58_17540

MAVU:

RE97_17575

MAV:

MAV_0365

MIT:

OCO_03200

MIA:

OCU_03280

MID:

MIP_00687

MYO:

OEM_03320

MCHI:

AN480_01635

MIR:

OCQ_03230

MMAL:

CKJ54_01535

MMAN:

MMAN_45770

MMM:

W7S_01575

MSHG:

MSG_01425

MXE:	MYXE_24180 MYXE_32280

MNV:	MNVI_24420

MSEO:

MSEO_36790

MHEK:

JMUB5695_02341 JMUB5695_03230

MBRD:

MBRA_38030

MMAM:

K3U93_21935

MMEH:

M5I08_17195

NBR:	O3I_017420

NNO:	NONO_c18400

NSR:	NS506_06814

NTP:	CRH09_10350 CRH09_21400

NOZ:	DMB37_16180 DMB37_39080

NAH:	F5544_20220

NWL:	NWFMUON74_53990

NTC:	KHQ06_35245

NHU:	H0264_34375

NYA:	LTV02_38570

NGP:	LTT66_36200

NSPU:

IFM12276_37540

NYU:	D7D52_09595

SMAL:

SMALA_6791 SMALA_7996

SSOI:

I1A49_37720 I1A49_43700

SDV:	BN159_0193

SALS:

SLNWT_6169 SLNWT_7198

STRC:

AA958_08245 AA958_19865

SPRI:

SPRI_0909

STRD:

NI25_02900

SAUO:

BV401_37550

SAST:

CD934_32625

SBAT:

G4Z16_01670

SFEU:

IM697_22255

SPEU:

CGZ69_03155 CGZ69_03170

STUB:

MMF93_32090

SLON:

LGI35_41685

SCOA:

QU709_45770

LEIF:

HF024_18140

MIO:	AOA12_07045

MCW:	A8L33_02660

MFOL:

DXT68_15640

MWA:	E4K62_17895

MPRT:

ET475_04985

MOY:	CVS54_03478

MLZ:	F6J85_17000

MRN:	K8F61_14735

MAZA:

NFX31_16065

RTN:	A6122_1315

RRY:	C1O28_13155

RFS:	C1I64_06665

FSB:	GCM10025867_36410

AARC:

G127AT_08370

AIL:	FLP10_14905

HUW:	FPZ11_15350

MANT:

BHD05_02530

HEH:	L3i23_22020

NAEI:

GCM126_20620

ARI:	UM93_01735

ACH:

Achl_4615

BCV:	Bcav_1697 Bcav_1706

BRX:	BH708_16840

BGG:	CFK41_13950

BKI:	M4486_17155

BRHA:

NLU66_17615

BHH:	Bra3105_13870

BHQ:	BRM3_01395

LMOI:

VV02_12665 VV02_16540

CELC:

K5O09_00775

PEI:	H9L10_00400

HALT:

IM660_11230 IM660_18690

RHAL:

LQF10_10845 LQF10_18330 LQF10_18375

RSUA:

LQF12_01490

PBO:	PACID_30190

AACI:

ASQ49_07055

ACIJ:

JS278_00348

MPH:

MLP_04200

MIK:	FOE78_16460

MICG:

GJV80_13020 GJV80_18715

TES:	BW730_09545

TDF:	H9L22_14135

NCA:

Noca_0057

SGRG:

L0C25_02255 L0C25_21200

KFL:	Kfla_1880 Kfla_2842

KQI:	F1D05_29155 F1D05_34720

KSL:	OG809_12245 OG809_19930 OG809_27280

ACTQ:

OG417_21435

NOW:	GBF35_30775 GBF35_30790

FRE:	Franean1_7052

SEN:	SACE_0944 SACE_2137

SACG:

FDZ84_00560 FDZ84_02430 FDZ84_16595

AMQ:	AMETH_1580 AMETH_5365

AACD:

LWP59_07960 LWP59_15895

ATHM:

L1857_10550 L1857_29795

KAL:

KALB_1788

KUT:	JJ691_23420

KPHY:

AOZ06_41090

SACE:

GIY23_03970 GIY23_09395

ACTY:

OG774_21350

MIL:

ML5_4285

MICB:

MicB006_5001

MTUA:

CSH63_14990

MTEM:

GCE86_08490

MPRN:

Q3V37_27780

MHAW:

RMN56_29955

PLK:	CIK06_05645

ATL:	Athai_32740

ASER:

Asera_41910

SNA:	Snas_3124 Snas_3258

BLJ:	BLD_0908 BLD_1491

BLN:

Blon_0046

BLON:

BLIJ_0046

BLL:

BLJ_1995

BLB:	BBMN68_912

BLM:	BLLJ_0461 BLLJ_1877

BLK:	BLNIAS_02101 BLNIAS_02937

BLG:

BIL_13830

BLZ:	BLGT_03120

BAD:

BAD_1588

BADL:

BADO_1694

BADO:

BBMN23_1762

BDE:	BDP_2163 BDP_2171

BDN:	BBDE_2032 BBDE_2040

BBRU:

Bbr_0030 Bbr_1861

BBRE:

B12L_1799

BBRJ:

B7017_0039

BBRC:

B7019_2052

BBRS:

BS27_0038

BAST:

BAST_0334 BAST_1636

BCOR:

BCOR_0277

BKA:	AH68_09590

BKS:

BBKW_1875

BCAT:

BBCT_1571 BBCT_1607

BII:	BINDI_0214

BANG:

BBAG_0439

BPSC:

BBPC_1677

BSCA:

BBSC_2504

BGX:	ESN35_02395

BLEM:

BL8807_03690

BEU:	BE0216_08370

BFJ:	B19861_18280

SIJ:

SCIP_0372

AEY:	CDG81_18140

RUB:	GBA63_16980

RRS:	RoseRS_2835

RCA:

Rcas_2768

ABAT:

CFX1CAM_1986

ABAO:

BEQ56_10230

CAP:	CLDAP_18720

KBS:	EPA93_23005

TTR:

Tter_0622

PMET:

G4Y79_06430

DPD:	Deipe_1359

DMB:	E5F05_00835

CCZ:	CCALI_00630 CCALI_02654

FGI:	OP10G_0366 OP10G_0633 OP10G_1344 OP10G_1487 OP10G_2091 OP10G_2798 OP10G_3516 OP10G_4487 OP10G_4808

NPY:	NPRO_01100 NPRO_09310 NPRO_13160 NPRO_17100

CCOT:

CCAX7_007310 CCAX7_41300

OTE:

Oter_3336

OLE:	K0B96_05115

ELUT:

CKA38_01365 CKA38_01545

PUO:	RZN69_00100 RZN69_14250 RZN69_15685 RZN69_20080

LUO:	HHL09_04820 HHL09_23865

VEG:	SAMN05444156_3178

FPES:

NXS98_01335 NXS98_02995 NXS98_08235 NXS98_10525

VBS:	EGM51_07940

MFF:	MFFC18_48150

AHEL:

Q31a_41490

AAGG:

ETAA8_16750

SNEP:

Enr13x_09610

TTF:	THTE_0699 THTE_4099 THTE_4165

AMUC:

Pan181_51430

PND:	Pla175_30480 Pla175_30490

AMOB:

HG15A2_17030 HG15A2_17040 HG15A2_43250

BMEI:

Spa11_22390 Spa11_25410

PLH:	VT85_24900

CCOP:

Mal65_32010

PBOR:

BSF38_01516 BSF38_02944 BSF38_03509

AGV:	OJF2_26270 OJF2_26860 OJF2_34730 OJF2_45230 OJF2_56740 OJF2_74770 OJF2_74890 OJF2_74900 OJF2_74920

PCOR:

KS4_24870

MCAD:

Pan265_12730

PBU:	L21SP3_01635

PBP:	STSP1_01648

PBAS:

SMSP2_00767 SMSP2_01913 SMSP2_01922 SMSP2_01961 SMSP2_02222

VBL:	L21SP4_01796

TAER:

GT409_00860

VBC:	C5Q97_00790

SCC:	Spico_0498

TAZ:	TREAZ_2028

BHC:	JFL75_02760 JFL75_15860 JFL75_15865

LBI:	LEPBI_I3383

LBF:

LBF_3269

LKB:	LPTSP3_g35250

TALB:

FTW19_07950

ABAS:

ACPOL_5317 ACPOL_7173 ACPOL_7178

EDA:	GWR55_11120

EDG:	H7846_07095

ADIN:

H7849_02605

ORP:	MOP44_00160 MOP44_21025

SUS:

Acid_0831

PFER:

IRI77_03600 IRI77_20605 IRI77_29325 IRI77_34035

ABAC:

LuPra_01197 LuPra_02560

LUB:	TBR22_A11450

GBA:

J421_2137

BFR:	BF4284 BF4528

BFS:	BF9343_3982 BF9343_4210

BFG:	BF638R_4141 BF638R_4481

BFB:	VU15_19745 VU15_21040

BXY:

BXY_21080

BOA:	Bovatus_03046

BCEL:

BcellWH2_03056

BIS:	DXK01_004630 DXK01_004705 DXK01_004710

BUN:	Bun01g_05090 Bun01g_05100 Bun01g_08130 Bun01g_08210 Bun01g_08310 Bun01g_08350 Bun01g_08380 Bun01g_17010 Bun01g_18160 Bun01g_19070

BFE:	K6V30_00845 K6V30_00855

BNO:	K8P02_12640

BHUM:

JXR92_004610

BSAL:

K6V25_19820

PBT:	ING2E5B_1386 ING2E5B_2253

PMUC:

ING2E5A_0263 ING2E5A_1581

PET:	PEIBARAKI_4034 PEIBARAKI_4041 PEIBARAKI_4042

PSAC:

PSM36_0007 PSM36_0014 PSM36_0015 PSM36_3154

PDI:

BDI_2465

PARC:

CI960_18575

PFAI:

NXY11_00585

COPR:

Cop2CBH44_30650

PHER:

prwr041_13200

PBUC:

LK429_02055 LK429_04690 LK429_04710

AFD:

Alfi_2406

ALD:	GFH31_01940

ADA:	A5CPEGH6_11540

AIT:	AI2BBH_03480

ASEN:

NQ519_12400

MCOS:

GM418_02430 GM418_10305 GM418_27615 GM418_28000

CPI:

Cpin_1790

CHIH:

GWR21_24380

COY:	HF329_03540

CFIL:

MYF79_09000

CHF:	KTO58_11100

CHOR:

MKQ68_14235

CSAC:

SIO70_10335

NSO:	NIASO_00510 NIASO_04895

NIA:	A8C56_12240 A8C56_13070 A8C56_13080 A8C56_13460 A8C56_13480 A8C56_13485 A8C56_13520 A8C56_18715

NYN:	U0035_13780 U0035_13790

AGI:	FSB73_14190

ATEE:

K9M52_00205

PGIN:

FRZ67_04840 FRZ67_21895

PGO:	FSB84_13135

FLS:	GLV81_00870

FLC:	KJS93_07945 KJS93_11455 KJS93_12190

THEO:

IMW88_03500

HHY:	Halhy_0382 Halhy_0604

PGS:	CPT03_05790 CPT03_06660 CPT03_20380

PMUO:

LOK61_16245

SHG:	Sph21_0614

SMIZ:

4412673_01333 4412673_02494

SPHZ:

E3D81_06100

SDJ:	NCTC13534_01556 NCTC13534_02086

STHA:

NCTC11429_00385

MUC:

MuYL_3107

MGOT:

MgSA37_02347

MUH:	HYN43_020445

MGIN:

FRZ54_12220 FRZ54_15320

MGK:	FSB76_16450

MRUB:

DEO27_020560

MGOS:

DIU38_021555

MSAB:

SNE25_19230 SNE25_19250 SNE25_19255 SNE25_19290 SNE25_23305 SNE25_31440

OLI:	FKG96_21740 FKG96_26545

CAMU:

CA2015_2173

EST:	DN752_22495

SRD:	SD10_01255

SMON:

AWR27_18380

FIB:	A6C57_16680

HYJ:	FHG12_05930

HQI:	H9L05_04620

PKO:	PKOR_14010

CLIT:

OQ292_05575

FBT:	D770_23785

CHK:	D4L85_03080 D4L85_30070

FAX:	FUAX_07660 FUAX_25980 FUAX_46000 FUAX_47770

ATK:	AUTU_33110

GFL:

GRFL_0465

ZPR:

ZPR_0900

ZGA:	ZOBELLIA_3462

PUN:	NQP51_12140

WIJ:	BWZ20_14355

AHZ:	APS56_06015

SALT:

AO058_17405 AO058_17440 AO058_17450 AO058_17485 AO058_17490 AO058_17515

FEK:	C1H87_02660

FLG:	LV716_14215

SPON:

HME9304_01956 HME9304_01957

PHY:	AJ81_06220

MPG:	Theba_1589

MINF:

MESINF_2411

ASAC:

ATHSA_0371

ALAM:

RT761_01354 RT761_01358 RT761_01361

MAMA:

GII36_05205

SBAG:

UW38_C0001G0591

FSH:	Q2T83_08945 Q2T83_15665

HRR:	HZS55_14600

CMA:

Cmaq_0234

LOKI:

Lokiarch_33310

LOB:	NEF87_002911

PSYT:

DSAG12_00869

» show all

Reference

1 [PMID:37076525]

Authors

Al-Jourani O, Benedict ST, Ross J, Layton AJ, van der Peet P, Marando VM, Bailey NP, Heunis T, Manion J, Mensitieri F, Franklin A, Abellon-Ruiz J, Oram SL, Parsons L, Cartmell A, Wright GSA, Basle A, Trost M, Henrissat B, Munoz-Munoz J, Hirt RP, Kiessling LL, Lovering AL, Williams SJ, Lowe EC, Moynihan PJ.

Title

Identification of D-arabinan-degrading enzymes in mycobacteria.

Journal

Nat Commun 14:2233 (2023)
DOI:10.1038/s41467-023-37839-5

Sequence

[nbr:O3I_017420] [mama:GII36_05205]

Reference

2 [PMID:37726269]

Authors

Shimokawa M, Ishiwata A, Kashima T, Nakashima C, Li J, Fukushima R, Sawai N, Nakamori M, Tanaka Y, Kudo A, Morikami S, Iwanaga N, Akai G, Shimizu N, Arakawa T, Yamada C, Kitahara K, Tanaka K, Ito Y, Fushinobu S, Fujita K.

Title

Identification and characterization of endo-alpha-, exo-alpha-, and exo-beta-D-arabinofuranosidases degrading lipoarabinomannan and arabinogalactan of mycobacteria.

Journal

Nat Commun 14:5803 (2023)
DOI:10.1038/s41467-023-41431-2

Sequence

[ag:GLC86735]

Other DBs

ExplorEnz - The Enzyme Database:

3.2.1.225

IUBMB Enzyme Nomenclature:

3.2.1.225

ExPASy - ENZYME nomenclature database:

3.2.1.225

BRENDA, the Enzyme Database:

3.2.1.225

LinkDB

DBGET integrated database retrieval system