| Entry |
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| Name |
phenylacetate 2-hydroxylase;
CYP504;
phaA (gene name)
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
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| Sysname |
phenylacetate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2-hydroxylating)
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| Reaction(IUBMB) |
phenylacetate + [reduced NADPH---hemoprotein reductase] + O2 = (2-hydroxyphenyl)acetate + [oxidized NADPH---hemoprotein reductase] + H2O [RN: R05487]
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| Reaction(KEGG) |
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| Substrate |
phenylacetate [CPD: C07086];
[reduced NADPH---hemoprotein reductase] [CPD: C03024];
O2 [CPD: C00007]
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| Product |
2-hydroxyphenylacetate [CPD: C05852];
[oxidized NADPH---hemoprotein reductase] [CPD: C03161];
H2O [CPD: C00001]
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| Comment |
This cytochrome P-450 (heme-thiolate) enzyme, found in Aspergillus nidulans, is involved in the degradation of phenylacetate.
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| History |
EC 1.14.14.54 created 2017
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| Pathway |
| ec01120 | Microbial metabolism in diverse environments |
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| Orthology |
| K10437 | phenylacetate 2-hydroxylase |
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| Genes |
» show all
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| Reference |
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| Authors |
Mingot JM, Penalva MA, Fernandez-Canon JM |
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| Title |
Disruption of phacA, an Aspergillus nidulans gene encoding a novel cytochrome P450 monooxygenase catalyzing phenylacetate 2-hydroxylation, results in penicillin overproduction. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Rodriguez-Saiz M, Barredo JL, Moreno MA, Fernandez-Canon JM, Penalva MA, Diez B. |
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| Title |
Reduced function of a phenylacetate-oxidizing cytochrome p450 caused strong genetic improvement in early phylogeny of penicillin-producing strains. |
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| Journal |
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| Sequence |
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| Other DBs |
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| LinkDB |
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