KEGG   ORTHOLOGY: K10437
Entry
K10437                      KO                                     

Name
PHAA, PHACA, CYP504A1
Definition
phenylacetate 2-hydroxylase [EC:1.14.14.54]
Pathway
ko00360  Phenylalanine metabolism
ko00643  Styrene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00360 Phenylalanine metabolism
    K10437  PHAA, PHACA, CYP504A1; phenylacetate 2-hydroxylase
  09111 Xenobiotics biodegradation and metabolism
   00643 Styrene degradation
    K10437  PHAA, PHACA, CYP504A1; phenylacetate 2-hydroxylase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K10437  PHAA, PHACA, CYP504A1; phenylacetate 2-hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.54  phenylacetate 2-hydroxylase
     K10437  PHAA, PHACA, CYP504A1; phenylacetate 2-hydroxylase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, fungi type
  CYP504 family
   K10437  PHAA, PHACA, CYP504A1; phenylacetate 2-hydroxylase
Other DBs
RN: R05487
COG: COG2124
GO: 0018631
Genes
QSU: 112006183 112014358
PPA: PAS_chr3_0864
DHA: DEHA2D03168g
PIC: PICST_49761
PGU: PGUG_01534
SPAA: SPAPADRAFT_48604
CAL: CAALFM_C404360WA(CaO19.1411)
CTP: CTRG_03930
COT: CORT_0E04490
CDU: CD36_44030
MGR: MGG_04684
MAW: MAC_08608
MAJ: MAA_10366
CMT: CCM_02553
MBE: MBM_06099
ANI: AN8078.2
ANG: ANI_1_128144(An16g01030) ANI_1_1740104(An12g04210)
ABE: ARB_03198
TVE: TRV_03913
ZTR: MYCGRDRAFT_32609(CYP-22) MYCGRDRAFT_83782(CYP-33)
TASA: A1Q1_06336
AG: AAF00011(pahA)
 » show all
Reference
  Authors
Mingot JM, Penalva MA, Fernandez-Canon JM
  Title
Disruption of phacA, an Aspergillus nidulans gene encoding a novel cytochrome P450 monooxygenase catalyzing phenylacetate 2-hydroxylation, results in penicillin overproduction.
  Journal
J Biol Chem 274:14545-50 (1999)
DOI:10.1074/jbc.274.21.14545
  Sequence
[ani:AN8078.2]
Reference
  Authors
Rodriguez-Saiz M, Barredo JL, Moreno MA, Fernandez-Canon JM, Penalva MA, Diez B.
  Title
Reduced function of a phenylacetate-oxidizing cytochrome p450 caused strong genetic improvement in early phylogeny of penicillin-producing strains.
  Journal
J Bacteriol 183:5465-71 (2001)
DOI:10.1128/JB.183.19.5465-5471.2001
  Sequence
LinkDB

KEGG   ENZYME: 1.14.14.54
Entry
EC 1.14.14.54               Enzyme                                 

Name
phenylacetate 2-hydroxylase;
CYP504;
phaA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
phenylacetate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
phenylacetate + [reduced NADPH---hemoprotein reductase] + O2 = (2-hydroxyphenyl)acetate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R05487]
Reaction(KEGG)
R05487
Substrate
phenylacetate [CPD:C07086];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
2-hydroxyphenylacetate [CPD:C05852];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
This cytochrome P-450 (heme-thiolate) enzyme, found in Aspergillus nidulans, is involved in the degradation of phenylacetate.
History
EC 1.14.14.54 created 2017
Pathway
ec00360  Phenylalanine metabolism
ec00643  Styrene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K10437  phenylacetate 2-hydroxylase
Genes
QSU: 112006183 112014358
PPA: PAS_chr3_0864
DHA: DEHA2D03168g
PIC: PICST_49761
PGU: PGUG_01534
SPAA: SPAPADRAFT_48604
CAL: CAALFM_C404360WA(CaO19.1411)
CTP: CTRG_03930
COT: CORT_0E04490
CDU: CD36_44030
MGR: MGG_04684
MAW: MAC_08608
MAJ: MAA_10366
CMT: CCM_02553
MBE: MBM_06099
ANI: AN8078.2
ANG: ANI_1_128144(An16g01030) ANI_1_1740104(An12g04210)
ABE: ARB_03198
TVE: TRV_03913
ZTR: MYCGRDRAFT_32609(CYP-22) MYCGRDRAFT_83782(CYP-33)
TASA: A1Q1_06336
 » show all
Reference
1  [PMID:10329644]
  Authors
Mingot JM, Penalva MA, Fernandez-Canon JM
  Title
Disruption of phacA, an Aspergillus nidulans gene encoding a novel cytochrome P450 monooxygenase catalyzing phenylacetate 2-hydroxylation, results in penicillin overproduction.
  Journal
J Biol Chem 274:14545-50 (1999)
DOI:10.1074/jbc.274.21.14545
  Sequence
[ani:AN8078.2]
Reference
2  [PMID:11544206]
  Authors
Rodriguez-Saiz M, Barredo JL, Moreno MA, Fernandez-Canon JM, Penalva MA, Diez B.
  Title
Reduced function of a phenylacetate-oxidizing cytochrome p450 caused strong genetic improvement in early phylogeny of penicillin-producing strains.
  Journal
J Bacteriol 183:5465-71 (2001)
DOI:10.1128/JB.183.19.5465-5471.2001
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.54
IUBMB Enzyme Nomenclature: 1.14.14.54
ExPASy - ENZYME nomenclature database: 1.14.14.54
BRENDA, the Enzyme Database: 1.14.14.54
LinkDB

KEGG   REACTION: R05487
Entry
R05487                      Reaction                               

Name
phenylacetate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2-hydroxylating)
Definition
Phenylacetic acid + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> 2-Hydroxyphenylacetate + [Oxidized NADPH---hemoprotein reductase] + H2O
Equation
Reaction class
RC00046  C05852_C07086
Enzyme
Pathway
rn00360  Phenylalanine metabolism
rn00643  Styrene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Orthology
K10437  phenylacetate 2-hydroxylase [EC:1.14.14.54]
Other DBs
RHEA: 53395
LinkDB

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