KEGG   ORTHOLOGY: K11627
Entry
K11627                      KO                                     

Name
pylS
Definition
pyrrolysyl-tRNA synthetase [EC:6.1.1.26]
Pathway
ko00970  Aminoacyl-tRNA biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09120 Genetic Information Processing
  09122 Translation
   00970 Aminoacyl-tRNA biosynthesis
    K11627  pylS; pyrrolysyl-tRNA synthetase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01007 Amino acid related enzymes
    K11627  pylS; pyrrolysyl-tRNA synthetase
  09182 Protein families: genetic information processing
   03016 Transfer RNA biogenesis
    K11627  pylS; pyrrolysyl-tRNA synthetase
Enzymes [BR:ko01000]
 6. Ligases
  6.1  Forming carbon-oxygen bonds
   6.1.1  Ligases forming aminoacyl-tRNA and related compounds
    6.1.1.26  pyrrolysine---tRNAPyl ligase
     K11627  pylS; pyrrolysyl-tRNA synthetase
Amino acid related enzymes [BR:ko01007]
 Aminoacyl-tRNA synthetase
  Class II (A)
   K11627  pylS; pyrrolysyl-tRNA synthetase
Transfer RNA biogenesis [BR:ko03016]
 Prokaryotic type
  Aminoacyl-tRNA synthetases (AARSs)
   Other AARSs
    K11627  pylS; pyrrolysyl-tRNA synthetase
Other DBs
RN: R08578
GO: 0043767
Genes
MBA: Mbar_A0839
MBY: MSBRM_0155
MBW: MSBRW_0162
MBAR: MSBR2_2226
MBAK: MSBR3_2277
MAC: MA_0155
MMA: MM_1445
MMJ: MSMAS_3219
MMAC: MSMAC_3241
MVC: MSVAZ_3361
METM: MSMTP_3119
MTHR: MSTHT_1934
MTHE: MSTHC_1349
MHOR: MSHOH_3965
MBU: Mbur_2086
MMET: MCMEM_0285
MMH: Mmah_0283
MHAZ: BHR79_02105(pylS)
MPY: Mpsy_1721(pylS)
MZI: HWN40_00965(pylS)
 » show all
Reference
  Authors
Li WT, Mahapatra A, Longstaff DG, Bechtel J, Zhao G, Kang PT, Chan MK, Krzycki JA
  Title
Specificity of pyrrolysyl-tRNA synthetase for pyrrolysine and pyrrolysine analogs.
  Journal
J Mol Biol 385:1156-64 (2009)
DOI:10.1016/j.jmb.2008.11.032
Reference
  Authors
Polycarpo C, Ambrogelly A, Berube A, Winbush SM, McCloskey JA, Crain PF, Wood JL, Soll D
  Title
An aminoacyl-tRNA synthetase that specifically activates pyrrolysine.
  Journal
Proc Natl Acad Sci U S A 101:12450-4 (2004)
DOI:10.1073/pnas.0405362101
  Sequence
LinkDB

KEGG   ENZYME: 6.1.1.26
Entry
EC 6.1.1.26                 Enzyme                                 

Name
pyrrolysine---tRNAPyl ligase;
PylS;
pyrrolysyl-tRNA synthetase
Class
Ligases;
Forming carbon-oxygen bonds;
Ligases forming aminoacyl-tRNA and related compounds
Sysname
L-pyrrolysine:tRNAPyl ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-pyrrolysine + tRNAPyl = AMP + diphosphate + L-pyrrolysyl-tRNAPyl [RN:R08578]
Reaction(KEGG)
R08578
Substrate
ATP [CPD:C00002];
L-pyrrolysine [CPD:C16138];
tRNA(Pyl) [CPD:C16139]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-pyrrolysyl-tRNA(Pyl) [CPD:C17027]
Comment
In organisms such as Methanosarcina barkeri that incorporate the modified amino acid pyrrolysine (Pyl) into certain methylamine methyltransferases, an unusual tRNAPyl, with a CUA anticodon, can be charged directly with pyrrolysine by this class II aminoacyl---tRNA ligase. The enzyme is specific for pyrrolysine as substrate as it cannot be replaced by lysine or any of the other natural amino acids [1].
History
EC 6.1.1.26 created 2007
Pathway
ec00970  Aminoacyl-tRNA biosynthesis
Orthology
K11627  pyrrolysyl-tRNA synthetase
Genes
MBA: Mbar_A0839
MBY: MSBRM_0155
MBW: MSBRW_0162
MBAR: MSBR2_2226
MBAK: MSBR3_2277
MAC: MA_0155
MMA: MM_1445
MMJ: MSMAS_3219
MMAC: MSMAC_3241
MVC: MSVAZ_3361
METM: MSMTP_3119
MTHR: MSTHT_1934
MTHE: MSTHC_1349
MHOR: MSHOH_3965
MBU: Mbur_2086
MMET: MCMEM_0285
MMH: Mmah_0283
MHAZ: BHR79_02105(pylS)
MPY: Mpsy_1721(pylS)
MZI: HWN40_00965(pylS)
 » show all
Reference
1  [PMID:15329732]
  Authors
Blight SK, Larue RC, Mahapatra A, Longstaff DG, Chang E, Zhao G, Kang PT, Green-Church KB, Chan MK, Krzycki JA.
  Title
Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo.
  Journal
Nature 431:333-5 (2004)
DOI:10.1038/nature02895
  Sequence
Reference
2  [PMID:15314242]
  Authors
Polycarpo C, Ambrogelly A, Berube A, Winbush SM, McCloskey JA, Crain PF, Wood JL, Soll D
  Title
An aminoacyl-tRNA synthetase that specifically activates pyrrolysine.
  Journal
Proc Natl Acad Sci U S A 101:12450-4 (2004)
DOI:10.1073/pnas.0405362101
  Sequence
Reference
3  [PMID:15372017]
  Authors
Schimmel P, Beebe K.
  Title
Molecular biology: genetic code seizes pyrrolysine.
  Journal
Nature 431:257-8 (2004)
DOI:10.1038/431257a
Other DBs
ExplorEnz - The Enzyme Database: 6.1.1.26
IUBMB Enzyme Nomenclature: 6.1.1.26
ExPASy - ENZYME nomenclature database: 6.1.1.26
BRENDA, the Enzyme Database: 6.1.1.26
LinkDB

KEGG   REACTION: R08578
Entry
R08578                      Reaction                               

Name
L-pyrrolysine:tRNA(Pyl) ligase (AMP-forming)
Definition
ATP + L-Pyrrolysine + tRNA(Pyl) <=> AMP + Diphosphate + L-Pyrrolysyl-tRNA(Pyl)
Equation
Reaction class
RC00055  C16139_C17027
RC00523  C16138_C17027
Enzyme
Pathway
rn00970  Aminoacyl-tRNA biosynthesis
Orthology
K11627  pyrrolysyl-tRNA synthetase [EC:6.1.1.26]
Other DBs
RHEA: 19280
LinkDB

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